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De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

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‘Ventura' at three stages.
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f1: ‘Ventura' at three stages.

Mentions: In this study, celery (‘Ventura') leaves at three stages were collected and analyzed using Illumina paired-end sequencing technology. The leaves were qualitatively evaluated for lignin distribution using histochemistry and autofluorescence microscopy (Figure 1), and then anatomically characterized. We constructed a database of the transcriptome sequences in the ‘Ventura' leaves and then isolated cDNA sequences with highly homologous genes that encode lignin biosynthesis-related enzymes, including PAL, C4H, 4CL, HCT, C3H, CCoAOMT, CCR, CAD, F5H, COMT, and POD. The cDNA sequences were used to analyze the expression profiles of genes involved in lignin biosynthesis in celery by quantitative real-time PCR. Profiling the expression of genes that encode lignin biosynthesis-related enzymes and understanding lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery. The results of this study may serve as a guide to develop breeding strategies that improve celery quality.


De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

‘Ventura' at three stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317703&req=5

f1: ‘Ventura' at three stages.
Mentions: In this study, celery (‘Ventura') leaves at three stages were collected and analyzed using Illumina paired-end sequencing technology. The leaves were qualitatively evaluated for lignin distribution using histochemistry and autofluorescence microscopy (Figure 1), and then anatomically characterized. We constructed a database of the transcriptome sequences in the ‘Ventura' leaves and then isolated cDNA sequences with highly homologous genes that encode lignin biosynthesis-related enzymes, including PAL, C4H, 4CL, HCT, C3H, CCoAOMT, CCR, CAD, F5H, COMT, and POD. The cDNA sequences were used to analyze the expression profiles of genes involved in lignin biosynthesis in celery by quantitative real-time PCR. Profiling the expression of genes that encode lignin biosynthesis-related enzymes and understanding lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery. The results of this study may serve as a guide to develop breeding strategies that improve celery quality.

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

Show MeSH