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Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

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Effects of H2S on F-actin in root of WT, cpa, capb and prf3.(a) Distribution of F-actin was shown in untreated control plants, treated with 200 μM NaHS or with 100 μM GYY4137 for 6 h. Images shown are representative of each treatment. Scale bar = 10 μm. (b) Quantification of the relative F-actin levels. The amount of F-actin in untreated WT roots was normalized to 100% as the control. Data are mean values and SE (n > 25) in (a) and (b). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
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f6: Effects of H2S on F-actin in root of WT, cpa, capb and prf3.(a) Distribution of F-actin was shown in untreated control plants, treated with 200 μM NaHS or with 100 μM GYY4137 for 6 h. Images shown are representative of each treatment. Scale bar = 10 μm. (b) Quantification of the relative F-actin levels. The amount of F-actin in untreated WT roots was normalized to 100% as the control. Data are mean values and SE (n > 25) in (a) and (b). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).

Mentions: The actin cytoskeleton in eukaryotic cells is a highly organized and dynamic structure that plays a central role in numerous cellular processes, including intracellular transport, cell growth, and organelle positioning42. F-actin is known to affect the plasma membrane localization of PIN proteins, as the vesicle transport of PIN proteins depends on F-actin4344. We examined the effects of H2S on the subcellular localization of F-actin (stained with fluorescein phalloidin), and the actin network in root epidermal cells observed by confocal microscopy. As shown in Fig. 6a, we observed that the elongation zone of the root tip and the actin network displayed thick, often longitudinally oriented cables and finer, randomly arranged filaments in the WT control. Treatment with NaHS or GYY4137 for 6 h caused significant changes in the actin cytoskeletal organization. After treatment, the thick actin cables were absent and the percentage of occupancy of F-actin bundles decreased in each cell (Fig. 6a).


Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Effects of H2S on F-actin in root of WT, cpa, capb and prf3.(a) Distribution of F-actin was shown in untreated control plants, treated with 200 μM NaHS or with 100 μM GYY4137 for 6 h. Images shown are representative of each treatment. Scale bar = 10 μm. (b) Quantification of the relative F-actin levels. The amount of F-actin in untreated WT roots was normalized to 100% as the control. Data are mean values and SE (n > 25) in (a) and (b). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317700&req=5

f6: Effects of H2S on F-actin in root of WT, cpa, capb and prf3.(a) Distribution of F-actin was shown in untreated control plants, treated with 200 μM NaHS or with 100 μM GYY4137 for 6 h. Images shown are representative of each treatment. Scale bar = 10 μm. (b) Quantification of the relative F-actin levels. The amount of F-actin in untreated WT roots was normalized to 100% as the control. Data are mean values and SE (n > 25) in (a) and (b). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
Mentions: The actin cytoskeleton in eukaryotic cells is a highly organized and dynamic structure that plays a central role in numerous cellular processes, including intracellular transport, cell growth, and organelle positioning42. F-actin is known to affect the plasma membrane localization of PIN proteins, as the vesicle transport of PIN proteins depends on F-actin4344. We examined the effects of H2S on the subcellular localization of F-actin (stained with fluorescein phalloidin), and the actin network in root epidermal cells observed by confocal microscopy. As shown in Fig. 6a, we observed that the elongation zone of the root tip and the actin network displayed thick, often longitudinally oriented cables and finer, randomly arranged filaments in the WT control. Treatment with NaHS or GYY4137 for 6 h caused significant changes in the actin cytoskeletal organization. After treatment, the thick actin cables were absent and the percentage of occupancy of F-actin bundles decreased in each cell (Fig. 6a).

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Show MeSH
Related in: MedlinePlus