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Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

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H2S regulates polar auxin transport in WT Arabidopsis root.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after various treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
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f4: H2S regulates polar auxin transport in WT Arabidopsis root.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after various treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).

Mentions: The observed change in the DR5::GUS expression pattern implies that auxin transport might be altered by increasing the endogenous H2S levels. To assess this hypothesis, we tested the acropetal and basipetal auxin transport in the roots of WT samples using [3H]-IAA. We used the untreated seedlings as the normalized samples and defined their value as 100% (Fig. 4). The normalized data showed that treatment with NaHS or GYY4137 markedly altered the auxin transport. A drastic reduction in acropetal and basipetal auxin movement was detected following NaHS and GYY4137 treatment (Fig. 4a & b). In addition, both the acropetal and basipetal auxin transport were decreased by NAP (Fig. 4a & b). These data supported the hypothesis that enhanced H2S levels cause a defect in the IAA transport capacity.


Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

H2S regulates polar auxin transport in WT Arabidopsis root.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after various treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317700&req=5

f4: H2S regulates polar auxin transport in WT Arabidopsis root.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after various treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
Mentions: The observed change in the DR5::GUS expression pattern implies that auxin transport might be altered by increasing the endogenous H2S levels. To assess this hypothesis, we tested the acropetal and basipetal auxin transport in the roots of WT samples using [3H]-IAA. We used the untreated seedlings as the normalized samples and defined their value as 100% (Fig. 4). The normalized data showed that treatment with NaHS or GYY4137 markedly altered the auxin transport. A drastic reduction in acropetal and basipetal auxin movement was detected following NaHS and GYY4137 treatment (Fig. 4a & b). In addition, both the acropetal and basipetal auxin transport were decreased by NAP (Fig. 4a & b). These data supported the hypothesis that enhanced H2S levels cause a defect in the IAA transport capacity.

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Show MeSH
Related in: MedlinePlus