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Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

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Effects of H2S on the auxin level of Arabidopsis root monitored by DR5::GUS.(a) Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. Scale bar = 100 μm. (b) GUS activity of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. (c) GUS activity of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. (d) Expression pattern of DR5::GUS in LRP. 5-d-old Arabidopsis DR5::GUS seedlings were grown on agar plates supplied with solvent (Control) or 200 μM NaHS for 0–48 h. Images shown are representative of each treatment. Scale bar = 50 μm. Mean values and SE are calculated from three replicates in (b) and (c). Data are mean values and SE (n > 25) in (a) and (d). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
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f3: Effects of H2S on the auxin level of Arabidopsis root monitored by DR5::GUS.(a) Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. Scale bar = 100 μm. (b) GUS activity of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. (c) GUS activity of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. (d) Expression pattern of DR5::GUS in LRP. 5-d-old Arabidopsis DR5::GUS seedlings were grown on agar plates supplied with solvent (Control) or 200 μM NaHS for 0–48 h. Images shown are representative of each treatment. Scale bar = 50 μm. Mean values and SE are calculated from three replicates in (b) and (c). Data are mean values and SE (n > 25) in (a) and (d). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).

Mentions: Auxin response patterns based on auxin gradients are important factors in the regulation of many developmental processes, including cell division, elongation, and differentiation during primary root growth6. As an indicator of the auxin response, DR5::GUS is expressed in the root apex and during LR initiation13. DR5::GUS expression was assayed by histochemical staining and a quantitative assay of GUS activity. The control showed that DR5::GUS is expressed in the quiescent center (QC), columella initial cells and mature columella cells of the root apex (Fig. 3a). DR5::GUS expression could be attenuated in the three layers of columella cells and confined to the QC by increasing the H2S levels after application of NaHS (Fig. 3a). After treatment with 200 μM NaHS or 100 μM GYY4137 for 12 or 24 h, the expression of DR5::GUS gene was also markedly inhibited (Fig. 3a). To further validate our results, the GUS activity was quantified. As shown in Fig. 3b & c, the GUS activity decreased in a dose-dependent and time-dependent manner after NaHS application. In addition, GYY4137 had the same effect as NaHS on the GUS activity.


Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Effects of H2S on the auxin level of Arabidopsis root monitored by DR5::GUS.(a) Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. Scale bar = 100 μm. (b) GUS activity of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. (c) GUS activity of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. (d) Expression pattern of DR5::GUS in LRP. 5-d-old Arabidopsis DR5::GUS seedlings were grown on agar plates supplied with solvent (Control) or 200 μM NaHS for 0–48 h. Images shown are representative of each treatment. Scale bar = 50 μm. Mean values and SE are calculated from three replicates in (b) and (c). Data are mean values and SE (n > 25) in (a) and (d). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317700&req=5

f3: Effects of H2S on the auxin level of Arabidopsis root monitored by DR5::GUS.(a) Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. Scale bar = 100 μm. (b) GUS activity of DR5::GUS in 5-d-old seedlings treated with 100–500 μM NaHS for 12 h. (c) GUS activity of DR5::GUS in 5-d-old seedlings treated with 200 μM NaHS and 100 μM GYY4137 for various time. (d) Expression pattern of DR5::GUS in LRP. 5-d-old Arabidopsis DR5::GUS seedlings were grown on agar plates supplied with solvent (Control) or 200 μM NaHS for 0–48 h. Images shown are representative of each treatment. Scale bar = 50 μm. Mean values and SE are calculated from three replicates in (b) and (c). Data are mean values and SE (n > 25) in (a) and (d). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
Mentions: Auxin response patterns based on auxin gradients are important factors in the regulation of many developmental processes, including cell division, elongation, and differentiation during primary root growth6. As an indicator of the auxin response, DR5::GUS is expressed in the root apex and during LR initiation13. DR5::GUS expression was assayed by histochemical staining and a quantitative assay of GUS activity. The control showed that DR5::GUS is expressed in the quiescent center (QC), columella initial cells and mature columella cells of the root apex (Fig. 3a). DR5::GUS expression could be attenuated in the three layers of columella cells and confined to the QC by increasing the H2S levels after application of NaHS (Fig. 3a). After treatment with 200 μM NaHS or 100 μM GYY4137 for 12 or 24 h, the expression of DR5::GUS gene was also markedly inhibited (Fig. 3a). To further validate our results, the GUS activity was quantified. As shown in Fig. 3b & c, the GUS activity decreased in a dose-dependent and time-dependent manner after NaHS application. In addition, GYY4137 had the same effect as NaHS on the GUS activity.

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Show MeSH
Related in: MedlinePlus