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Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

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H2S modulates polar auxin transport in root of WT, cpa, capb and prf3.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after NaHS treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
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f10: H2S modulates polar auxin transport in root of WT, cpa, capb and prf3.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after NaHS treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).

Mentions: The acropetal and basipetal auxin transport rates were decreased in the cpb compared to the WT root samples (Fig. 10). In the roots of cpa, the basipetal auxin transport rates were decreased in 2 to 4 mm (Fig. 10). prf3 displayed a similar auxin transport capacity as WT (Fig. 10). H2S could observably decrease the auxin transport rates in the WT sample (Fig. 4). Interestingly, there was an obvious difference between the mutant and WT plants after treatment with NaHS for 12 h. In the root of cpb, the effect of NaHS was removed in the acropetal auxin transport in both 0 to 2 mm and 2 to 4 mm, and in the basipetal auxin transport in 2 to 4 mm (Fig. 10). The weak auxin transport capacity was partially recovered compared with the WT levels in cpa and prf3 after NaHS treatment for 12 h (Fig. 10). The H2S-induced inhibition of the primary root length was markedly removed in the cpa, capb and prf3 mutant plants under 100 μM NaHS treatment and partly removed in capb and prf3 under 200 μM NaHS (Fig. S4 and S5). These data suggest that CPA, CPB and PRF3 play important roles in H2S by regulating auxin transport.


Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

H2S modulates polar auxin transport in root of WT, cpa, capb and prf3.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after NaHS treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317700&req=5

f10: H2S modulates polar auxin transport in root of WT, cpa, capb and prf3.Root acropetal auxin transport (a), and basipetal auxin transport (b) were assayed after NaHS treatments for 12 h in 5-d-old seedlings. Mean values and SE are calculated from three replicates. Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
Mentions: The acropetal and basipetal auxin transport rates were decreased in the cpb compared to the WT root samples (Fig. 10). In the roots of cpa, the basipetal auxin transport rates were decreased in 2 to 4 mm (Fig. 10). prf3 displayed a similar auxin transport capacity as WT (Fig. 10). H2S could observably decrease the auxin transport rates in the WT sample (Fig. 4). Interestingly, there was an obvious difference between the mutant and WT plants after treatment with NaHS for 12 h. In the root of cpb, the effect of NaHS was removed in the acropetal auxin transport in both 0 to 2 mm and 2 to 4 mm, and in the basipetal auxin transport in 2 to 4 mm (Fig. 10). The weak auxin transport capacity was partially recovered compared with the WT levels in cpa and prf3 after NaHS treatment for 12 h (Fig. 10). The H2S-induced inhibition of the primary root length was markedly removed in the cpa, capb and prf3 mutant plants under 100 μM NaHS treatment and partly removed in capb and prf3 under 200 μM NaHS (Fig. S4 and S5). These data suggest that CPA, CPB and PRF3 play important roles in H2S by regulating auxin transport.

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Show MeSH
Related in: MedlinePlus