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Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

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Effects of NaSH on endogenous H2S content in the root of WT Arabidopsis.(a) 5-d-old Arabidopsis seedlings were transferred to the 1/2 MS agar medium. Effects of NaSH on endogenous H2S content in Arabidopsis root. 50–1000 μM NaHS were used for various treatments for 6 h. (b) Effect of H2S in the regulation of primary root growth in WT Arabidopsis. Photograph showing the length of primary root of WT Arabidopsis seedlings. 3-d-old seedlings were transferred to 1/2 MS agar plates grown for 2 d. The agar plate were untreated (Control) or supplemented with 50 μM, 100 μM, 200 μM, or 500 μM of NaHS or supplemented with 200 μM GYY4137. Scale bar = 1 cm. (c) The length of primary root were obtained 2 d after the treatment of 3-d-old seedlings. Mean values and SE are calculated from three replicates in (a). Data are mean values and SE (n > 25) in (b) and (c). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
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f1: Effects of NaSH on endogenous H2S content in the root of WT Arabidopsis.(a) 5-d-old Arabidopsis seedlings were transferred to the 1/2 MS agar medium. Effects of NaSH on endogenous H2S content in Arabidopsis root. 50–1000 μM NaHS were used for various treatments for 6 h. (b) Effect of H2S in the regulation of primary root growth in WT Arabidopsis. Photograph showing the length of primary root of WT Arabidopsis seedlings. 3-d-old seedlings were transferred to 1/2 MS agar plates grown for 2 d. The agar plate were untreated (Control) or supplemented with 50 μM, 100 μM, 200 μM, or 500 μM of NaHS or supplemented with 200 μM GYY4137. Scale bar = 1 cm. (c) The length of primary root were obtained 2 d after the treatment of 3-d-old seedlings. Mean values and SE are calculated from three replicates in (a). Data are mean values and SE (n > 25) in (b) and (c). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).

Mentions: It has been indicated that H2S is a secondary signal molecule that acts in response to the growth and development of plants2526. The exogenous application of H2S donors was able to alter the endogenous H2S levels in a dose-dependent manner in Arabidopsis roots (Fig. 1a) and in other plants, such as maize and strawberry2129. Changing the endogenous H2S levels can affect the root system architecture36. However, our knowledge of the molecular mechanisms by which way H2S regulates growth and development in Arabidopsis remains fragmentary.


Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.

Jia H, Hu Y, Fan T, Li J - Sci Rep (2015)

Effects of NaSH on endogenous H2S content in the root of WT Arabidopsis.(a) 5-d-old Arabidopsis seedlings were transferred to the 1/2 MS agar medium. Effects of NaSH on endogenous H2S content in Arabidopsis root. 50–1000 μM NaHS were used for various treatments for 6 h. (b) Effect of H2S in the regulation of primary root growth in WT Arabidopsis. Photograph showing the length of primary root of WT Arabidopsis seedlings. 3-d-old seedlings were transferred to 1/2 MS agar plates grown for 2 d. The agar plate were untreated (Control) or supplemented with 50 μM, 100 μM, 200 μM, or 500 μM of NaHS or supplemented with 200 μM GYY4137. Scale bar = 1 cm. (c) The length of primary root were obtained 2 d after the treatment of 3-d-old seedlings. Mean values and SE are calculated from three replicates in (a). Data are mean values and SE (n > 25) in (b) and (c). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317700&req=5

f1: Effects of NaSH on endogenous H2S content in the root of WT Arabidopsis.(a) 5-d-old Arabidopsis seedlings were transferred to the 1/2 MS agar medium. Effects of NaSH on endogenous H2S content in Arabidopsis root. 50–1000 μM NaHS were used for various treatments for 6 h. (b) Effect of H2S in the regulation of primary root growth in WT Arabidopsis. Photograph showing the length of primary root of WT Arabidopsis seedlings. 3-d-old seedlings were transferred to 1/2 MS agar plates grown for 2 d. The agar plate were untreated (Control) or supplemented with 50 μM, 100 μM, 200 μM, or 500 μM of NaHS or supplemented with 200 μM GYY4137. Scale bar = 1 cm. (c) The length of primary root were obtained 2 d after the treatment of 3-d-old seedlings. Mean values and SE are calculated from three replicates in (a). Data are mean values and SE (n > 25) in (b) and (c). Within each set of experiments, bars with different letters are significantly different (P < 0.05, Duncan's multiple range tests).
Mentions: It has been indicated that H2S is a secondary signal molecule that acts in response to the growth and development of plants2526. The exogenous application of H2S donors was able to alter the endogenous H2S levels in a dose-dependent manner in Arabidopsis roots (Fig. 1a) and in other plants, such as maize and strawberry2129. Changing the endogenous H2S levels can affect the root system architecture36. However, our knowledge of the molecular mechanisms by which way H2S regulates growth and development in Arabidopsis remains fragmentary.

Bottom Line: H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots.Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells.In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.

Show MeSH
Related in: MedlinePlus