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Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation.

Chen C, Li H, Kong X, Zhang SM, Lee IS - Int J Nanomedicine (2014)

Bottom Line: Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone.The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation.Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants.

View Article: PubMed Central - PubMed

Affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, People's Republic of China ; Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China.

ABSTRACT
In this study, to improve the osseointegration of implants, osteogenic growth peptide (OGP) and fibronectin (FN) were loaded within mineral, which was formed on titanium, through adsorption and coprecipitation methods. The release profiles of OGP loaded by either adsorption or coprecipitation and the effects of the loading methods to immobilize OGP with and without FN on rat mesenchymal stem cell (rMSC) osteogenic differentiation were studied. The coprecipitation approach slightly reduced the initial burst release, while the adsorption approach provided a more sustained release. Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone. Dually loaded OGP and FN also had a positive impact on rMSC proliferation and osteogenic differentiation. The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation. Compared with coprecipitated OGP alone, adsorbed OGP enhanced later differentiation, such as osteocalcin secretion and matrix mineralization. Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants.

No MeSH data available.


Related in: MedlinePlus

In vitro cumulative release of FITC-OGP in PBS over the 14-day time period.Notes: Samples were prepared via 24-hour mineralization, 24-hour coprecipitation of mineral and FITC-OGP (cO) and 24-hour mineralization, FITC-OGP adsorption (aO). The values represent the mean ± standard deviation (n=3).Abbreviations: FITC-OGP, fluorescein isothiocyanate-labeled OGP; OGP, osteogenic growth peptide; PBS, phosphate-buffered saline.
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f4-ijn-10-283: In vitro cumulative release of FITC-OGP in PBS over the 14-day time period.Notes: Samples were prepared via 24-hour mineralization, 24-hour coprecipitation of mineral and FITC-OGP (cO) and 24-hour mineralization, FITC-OGP adsorption (aO). The values represent the mean ± standard deviation (n=3).Abbreviations: FITC-OGP, fluorescein isothiocyanate-labeled OGP; OGP, osteogenic growth peptide; PBS, phosphate-buffered saline.

Mentions: The amount of coprecipitated or adsorbed FITC-OGP was approximately 5 μg per sample. The release behaviors of FITC-OGP from prepared substrates are depicted in Figure 4. In general, the OGP release for both loading methods followed biphasic release kinetics. The cO group released approximately 58.74%±4.10% of the FITC-OGP during the initial 24 hours, after which the release rate leveled off. Over a period of 15 days, 68.82%±4.84% of the FITC-OGP was released from the coprecipitated surfaces. The amount of FITC-OGP released from the aO group on the first day was approximately 62.88%±5.28%, which was followed by a more gradual and steady release, at 1.5%/day up to 15 days, totaling 84.49%±8.13%.


Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation.

Chen C, Li H, Kong X, Zhang SM, Lee IS - Int J Nanomedicine (2014)

In vitro cumulative release of FITC-OGP in PBS over the 14-day time period.Notes: Samples were prepared via 24-hour mineralization, 24-hour coprecipitation of mineral and FITC-OGP (cO) and 24-hour mineralization, FITC-OGP adsorption (aO). The values represent the mean ± standard deviation (n=3).Abbreviations: FITC-OGP, fluorescein isothiocyanate-labeled OGP; OGP, osteogenic growth peptide; PBS, phosphate-buffered saline.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317146&req=5

f4-ijn-10-283: In vitro cumulative release of FITC-OGP in PBS over the 14-day time period.Notes: Samples were prepared via 24-hour mineralization, 24-hour coprecipitation of mineral and FITC-OGP (cO) and 24-hour mineralization, FITC-OGP adsorption (aO). The values represent the mean ± standard deviation (n=3).Abbreviations: FITC-OGP, fluorescein isothiocyanate-labeled OGP; OGP, osteogenic growth peptide; PBS, phosphate-buffered saline.
Mentions: The amount of coprecipitated or adsorbed FITC-OGP was approximately 5 μg per sample. The release behaviors of FITC-OGP from prepared substrates are depicted in Figure 4. In general, the OGP release for both loading methods followed biphasic release kinetics. The cO group released approximately 58.74%±4.10% of the FITC-OGP during the initial 24 hours, after which the release rate leveled off. Over a period of 15 days, 68.82%±4.84% of the FITC-OGP was released from the coprecipitated surfaces. The amount of FITC-OGP released from the aO group on the first day was approximately 62.88%±5.28%, which was followed by a more gradual and steady release, at 1.5%/day up to 15 days, totaling 84.49%±8.13%.

Bottom Line: Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone.The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation.Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants.

View Article: PubMed Central - PubMed

Affiliation: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, People's Republic of China ; Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China.

ABSTRACT
In this study, to improve the osseointegration of implants, osteogenic growth peptide (OGP) and fibronectin (FN) were loaded within mineral, which was formed on titanium, through adsorption and coprecipitation methods. The release profiles of OGP loaded by either adsorption or coprecipitation and the effects of the loading methods to immobilize OGP with and without FN on rat mesenchymal stem cell (rMSC) osteogenic differentiation were studied. The coprecipitation approach slightly reduced the initial burst release, while the adsorption approach provided a more sustained release. Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone. Dually loaded OGP and FN also had a positive impact on rMSC proliferation and osteogenic differentiation. The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation. Compared with coprecipitated OGP alone, adsorbed OGP enhanced later differentiation, such as osteocalcin secretion and matrix mineralization. Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants.

No MeSH data available.


Related in: MedlinePlus