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Histopathological study of corneal flap striae following laser in situ keratomileusis in rabbits.

Liu L, Song FZ, Bao LY - Exp Ther Med (2015)

Bottom Line: In conclusion, the inflammatory reactions and clinical impact of flap macrostriae were severe.Macrostriae involving two-thirds of the entire cornea remained visible six months postoperatively.Longer-term studies are required to further elucidate the issues associated with corneal flap striae.

View Article: PubMed Central - PubMed

Affiliation: Research Center of Molecular Medicine and Cancer, Chongqing Medical University, Chongqing 400016, P.R. China.

ABSTRACT

The aim of the present study was to investigate the histopathological changes and wound healing process of rabbit corneas following conventional laser in situ keratomileusis (LASIK) with and without the complication of flap macrostriae. The right eyes of 14 rabbits underwent LASIK with the formation of flap striae (macrostriae group) and the left underwent LASIK alone (control group). Two rabbits were selected at random for sacrifice on days 1, 3, 7 and 14, and at 1, 3 and 6 months postoperatively. The histopathological characters of the corneas were compared by hematoxylin and eosin (H&E), periodic acid-Schiff (PAS) and Masson staining. In the control group, the epithelial basement membrane of the cornea exhibited microstriae and the arrangement of stromal collagen fibers was regular. The width of the microstriae in the flap was 20-40 μm one week after surgery and the microstriae were no longer visible two weeks postoperatively. In the macrostriae group, infiltration of polymorphonuclear cells occurred around the incision and irregular hyperplasia of the epithelium was observed due to undulation of the epithelial basement membrane on the first postoperative day. The collagen fibers and striae of the corneal stroma exhibited irregular undulation one month postoperatively. The area between the corneal flap and stromal bed was distinctly stained by PAS and Masson stains. Macrostriae with a width of 80-120 μm affecting two-thirds of the entire cornea remained visible six months postoperatively. In conclusion, the inflammatory reactions and clinical impact of flap macrostriae were severe. Macrostriae involving two-thirds of the entire cornea remained visible six months postoperatively. Longer-term studies are required to further elucidate the issues associated with corneal flap striae.

No MeSH data available.


Related in: MedlinePlus

Morphological characteristics of the normal cornea. (A) The corneal epithelium was flat with uniform thickness and the arrangement of stromal collagen fiber was regular (hematoxylin and eosin staining; magnification, ×200); (B) no positive staining of the normal cornea was observed with periodic acid-Schiff stain (magnification, ×200) or (C) Masson’s trichrome stain (magnification, ×200).
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f2-etm-09-03-0895: Morphological characteristics of the normal cornea. (A) The corneal epithelium was flat with uniform thickness and the arrangement of stromal collagen fiber was regular (hematoxylin and eosin staining; magnification, ×200); (B) no positive staining of the normal cornea was observed with periodic acid-Schiff stain (magnification, ×200) or (C) Masson’s trichrome stain (magnification, ×200).

Mentions: A normal cornea stained with H&E is shown in Fig. 2A. The corneal epithelium was flat with uniform thickness. The morphologies of the flat and columnar cells (~4–5 layers) could be observed clearly. The epithelial basement membrane was continuous and flat. The arrangement of stromal collagen fibers was regular and the nuclei of corneal stroma cells exhibited a fusiform shape. In Fig. 2B, a section of normal cornea stained with PAS is shown. Each layer of cells was stained evenly, with the exception of the posterior elastic layer and the endothelium of the cornea, which were stained deeply. A section of normal cornea stained with Masson’s trichrome stain is shown in Fig. 2C. The endothelium, nuclei of corneal stromal cells and endothelial cells were stained red while the remaining tissues were stained blue.


Histopathological study of corneal flap striae following laser in situ keratomileusis in rabbits.

Liu L, Song FZ, Bao LY - Exp Ther Med (2015)

Morphological characteristics of the normal cornea. (A) The corneal epithelium was flat with uniform thickness and the arrangement of stromal collagen fiber was regular (hematoxylin and eosin staining; magnification, ×200); (B) no positive staining of the normal cornea was observed with periodic acid-Schiff stain (magnification, ×200) or (C) Masson’s trichrome stain (magnification, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316991&req=5

f2-etm-09-03-0895: Morphological characteristics of the normal cornea. (A) The corneal epithelium was flat with uniform thickness and the arrangement of stromal collagen fiber was regular (hematoxylin and eosin staining; magnification, ×200); (B) no positive staining of the normal cornea was observed with periodic acid-Schiff stain (magnification, ×200) or (C) Masson’s trichrome stain (magnification, ×200).
Mentions: A normal cornea stained with H&E is shown in Fig. 2A. The corneal epithelium was flat with uniform thickness. The morphologies of the flat and columnar cells (~4–5 layers) could be observed clearly. The epithelial basement membrane was continuous and flat. The arrangement of stromal collagen fibers was regular and the nuclei of corneal stroma cells exhibited a fusiform shape. In Fig. 2B, a section of normal cornea stained with PAS is shown. Each layer of cells was stained evenly, with the exception of the posterior elastic layer and the endothelium of the cornea, which were stained deeply. A section of normal cornea stained with Masson’s trichrome stain is shown in Fig. 2C. The endothelium, nuclei of corneal stromal cells and endothelial cells were stained red while the remaining tissues were stained blue.

Bottom Line: In conclusion, the inflammatory reactions and clinical impact of flap macrostriae were severe.Macrostriae involving two-thirds of the entire cornea remained visible six months postoperatively.Longer-term studies are required to further elucidate the issues associated with corneal flap striae.

View Article: PubMed Central - PubMed

Affiliation: Research Center of Molecular Medicine and Cancer, Chongqing Medical University, Chongqing 400016, P.R. China.

ABSTRACT

The aim of the present study was to investigate the histopathological changes and wound healing process of rabbit corneas following conventional laser in situ keratomileusis (LASIK) with and without the complication of flap macrostriae. The right eyes of 14 rabbits underwent LASIK with the formation of flap striae (macrostriae group) and the left underwent LASIK alone (control group). Two rabbits were selected at random for sacrifice on days 1, 3, 7 and 14, and at 1, 3 and 6 months postoperatively. The histopathological characters of the corneas were compared by hematoxylin and eosin (H&E), periodic acid-Schiff (PAS) and Masson staining. In the control group, the epithelial basement membrane of the cornea exhibited microstriae and the arrangement of stromal collagen fibers was regular. The width of the microstriae in the flap was 20-40 μm one week after surgery and the microstriae were no longer visible two weeks postoperatively. In the macrostriae group, infiltration of polymorphonuclear cells occurred around the incision and irregular hyperplasia of the epithelium was observed due to undulation of the epithelial basement membrane on the first postoperative day. The collagen fibers and striae of the corneal stroma exhibited irregular undulation one month postoperatively. The area between the corneal flap and stromal bed was distinctly stained by PAS and Masson stains. Macrostriae with a width of 80-120 μm affecting two-thirds of the entire cornea remained visible six months postoperatively. In conclusion, the inflammatory reactions and clinical impact of flap macrostriae were severe. Macrostriae involving two-thirds of the entire cornea remained visible six months postoperatively. Longer-term studies are required to further elucidate the issues associated with corneal flap striae.

No MeSH data available.


Related in: MedlinePlus