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MicroRNA-124 functions as a tumor suppressor and indicates prognosis in human osteosarcoma.

Han G, Wang Y, Bi W, Jia J, Wang W - Exp Ther Med (2014)

Bottom Line: MicroRNA-124 (miR-124) has been demonstrated to be downregulated in numerous human malignancies and correlated with tumor progression.It was found that the expression levels of miR-124 in osteosarcoma tissues were significantly lower than those in corresponding noncancerous bone tissues (P<0.001).Univariate and multivariate analysis identified low miR-124 expression as an unfavorable prognostic factor for overall survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, General Hospital of PLA, Beijing 100853, P.R. China.

ABSTRACT

MicroRNA-124 (miR-124) has been demonstrated to be downregulated in numerous human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma remains unclear. Thus, the aim of the present study was to explore the effects of miR-124 in osteosarcoma tumorigenesis and development. Using reverse transcription-quantitative polymerase chain reaction, miR-124 expression was detected in primary osteosarcoma tissues and osteosarcoma cell lines. The correlation of miR-124 expression with clinicopathological factors and prognosis was statistically analyzed. MTT, flow cytometric, and Transwell invasion and migration assays were used to test the proliferation, apoptosis, invasion and migration of osteosarcoma cells transfected with miR-124 mimic. It was found that the expression levels of miR-124 in osteosarcoma tissues were significantly lower than those in corresponding noncancerous bone tissues (P<0.001). In addition, miR-124 downregulation more frequently occurred in osteosarcoma specimens with advanced clinical stage (P<0.001), positive distant metastasis (P=0.005) and poor response to neoadjuvant chemotherapy (P=0.013). Univariate and multivariate analysis identified low miR-124 expression as an unfavorable prognostic factor for overall survival. Furthermore, transfection of miR-124 mimic into MG63 cells was able to reduce cell proliferation, invasion and migration, and promote cell apoptosis. These findings indicate that miR-124 may act not only as a novel diagnostic and prognostic marker, but also as a potential target for the molecular therapy of osteosarcoma.

No MeSH data available.


Related in: MedlinePlus

Expression of miR-124 in osteosarcoma tissues and cell lines. (A) miR-124 expression was significantly lower in osteosarcoma tissues than in the corresponding nontumorous samples; (B) miR-124 expression was down-regulated in osteosarcoma cell lines MG63, U2OS, Saos-2, and SW1353, compared to human normal osteoblastic cell line hFOB 1.19. miR-124, microRNA-124.
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f1-etm-09-03-0679: Expression of miR-124 in osteosarcoma tissues and cell lines. (A) miR-124 expression was significantly lower in osteosarcoma tissues than in the corresponding nontumorous samples; (B) miR-124 expression was down-regulated in osteosarcoma cell lines MG63, U2OS, Saos-2, and SW1353, compared to human normal osteoblastic cell line hFOB 1.19. miR-124, microRNA-124.

Mentions: The expression levels of miR-124 in osteosarcoma tissues, corresponding noncancerous bone biopsy samples, osteosarcoma cell lines and the human normal osteoblastic cell line hFOB 1.19 were detected by RT-qPCR and normalized to U6 small nuclear RNA. As shown in Fig. 1A, the results revealed that miR-124 expression levels were significantly lower in osteosarcoma tissues (8.3±2.1) than in the corresponding noncancerous bone tissues (19.6±4.2; P<0.001). Decreased miR-124 expression was also observed in osteosarcoma cell lines compared with that in hFOB 1.19 cells (Fig. 1B; P<0.001). The MG63 cell line, which possessed the lowest levels of miR-124 expression among all tested osteosarcoma cell lines, was selected for analysis in further experiments.


MicroRNA-124 functions as a tumor suppressor and indicates prognosis in human osteosarcoma.

Han G, Wang Y, Bi W, Jia J, Wang W - Exp Ther Med (2014)

Expression of miR-124 in osteosarcoma tissues and cell lines. (A) miR-124 expression was significantly lower in osteosarcoma tissues than in the corresponding nontumorous samples; (B) miR-124 expression was down-regulated in osteosarcoma cell lines MG63, U2OS, Saos-2, and SW1353, compared to human normal osteoblastic cell line hFOB 1.19. miR-124, microRNA-124.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316987&req=5

f1-etm-09-03-0679: Expression of miR-124 in osteosarcoma tissues and cell lines. (A) miR-124 expression was significantly lower in osteosarcoma tissues than in the corresponding nontumorous samples; (B) miR-124 expression was down-regulated in osteosarcoma cell lines MG63, U2OS, Saos-2, and SW1353, compared to human normal osteoblastic cell line hFOB 1.19. miR-124, microRNA-124.
Mentions: The expression levels of miR-124 in osteosarcoma tissues, corresponding noncancerous bone biopsy samples, osteosarcoma cell lines and the human normal osteoblastic cell line hFOB 1.19 were detected by RT-qPCR and normalized to U6 small nuclear RNA. As shown in Fig. 1A, the results revealed that miR-124 expression levels were significantly lower in osteosarcoma tissues (8.3±2.1) than in the corresponding noncancerous bone tissues (19.6±4.2; P<0.001). Decreased miR-124 expression was also observed in osteosarcoma cell lines compared with that in hFOB 1.19 cells (Fig. 1B; P<0.001). The MG63 cell line, which possessed the lowest levels of miR-124 expression among all tested osteosarcoma cell lines, was selected for analysis in further experiments.

Bottom Line: MicroRNA-124 (miR-124) has been demonstrated to be downregulated in numerous human malignancies and correlated with tumor progression.It was found that the expression levels of miR-124 in osteosarcoma tissues were significantly lower than those in corresponding noncancerous bone tissues (P<0.001).Univariate and multivariate analysis identified low miR-124 expression as an unfavorable prognostic factor for overall survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, General Hospital of PLA, Beijing 100853, P.R. China.

ABSTRACT

MicroRNA-124 (miR-124) has been demonstrated to be downregulated in numerous human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma remains unclear. Thus, the aim of the present study was to explore the effects of miR-124 in osteosarcoma tumorigenesis and development. Using reverse transcription-quantitative polymerase chain reaction, miR-124 expression was detected in primary osteosarcoma tissues and osteosarcoma cell lines. The correlation of miR-124 expression with clinicopathological factors and prognosis was statistically analyzed. MTT, flow cytometric, and Transwell invasion and migration assays were used to test the proliferation, apoptosis, invasion and migration of osteosarcoma cells transfected with miR-124 mimic. It was found that the expression levels of miR-124 in osteosarcoma tissues were significantly lower than those in corresponding noncancerous bone tissues (P<0.001). In addition, miR-124 downregulation more frequently occurred in osteosarcoma specimens with advanced clinical stage (P<0.001), positive distant metastasis (P=0.005) and poor response to neoadjuvant chemotherapy (P=0.013). Univariate and multivariate analysis identified low miR-124 expression as an unfavorable prognostic factor for overall survival. Furthermore, transfection of miR-124 mimic into MG63 cells was able to reduce cell proliferation, invasion and migration, and promote cell apoptosis. These findings indicate that miR-124 may act not only as a novel diagnostic and prognostic marker, but also as a potential target for the molecular therapy of osteosarcoma.

No MeSH data available.


Related in: MedlinePlus