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Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia.

Deng CL, Ling ST, Liu XQ, Zhao YJ, Lv YF - Exp Ther Med (2015)

Bottom Line: The levels of MMP-1 in the umbilical serum of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 294.33±11.53, 247.78±20.32, 177.67±12.63 and 124.68±15.41 pg/ml, respectively, and there were significant differences between each two groups (P<0.05).However, no significant difference was identified between each two groups with regard to the levels of TIMP-1 in the umbilical cord and the positive rates in the placenta and decidua (P>0.05).Reduced MMP-1 levels in the umbilical serum, placenta and decidua were observed in women who developed preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynaecology and Obstetrics, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.

ABSTRACT

The aim of the present study was to explore the levels of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the maternal umbilical serum, placenta and decidua of patients with preeclampsia compared with those in normotensive pregnant females. A total of 73 pregnant females were recruited as the test subjects, including 43 inpatients with hypertensive disorders in pregnancy and 30 normal pregnant females as the control. The 43 inpatients with hypertensive disorders in pregnancy included 18 patients with gestational hypertension, nine with mild preeclampsia and 16 with severe preeclampsia. MMP-1 and TIMP-1 ELISA kits were used to determine the MMP-1 and TIMP-1 levels in the umbilical serum of the parturient following delivery. MMP-1 and TIMP-1 expressed in the placenta and decidua of the parturient following delivery were evaluated using immunohistochemistry. MMP-1 and TIMP-1 were mainly located in cytotrophoblasts and syncytiotrophoblasts in the placenta and decidua. The levels of MMP-1 in the umbilical serum of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 294.33±11.53, 247.78±20.32, 177.67±12.63 and 124.68±15.41 pg/ml, respectively, and there were significant differences between each two groups (P<0.05). The positive expression rate of MMP-1 in the placenta and decidua of patients with hypertensive disorders in pregnancy was lower than that of the controls (P<0.01 and P<0.01, respectively). However, no significant difference was identified between each two groups with regard to the levels of TIMP-1 in the umbilical cord and the positive rates in the placenta and decidua (P>0.05). Reduced MMP-1 levels in the umbilical serum, placenta and decidua were observed in women who developed preeclampsia.

No MeSH data available.


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Immunohistochemical staining for matrix metalloproteinase-1 in the placenta of a patient with severe preeclampsia. Magnification, ×400.
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f4-etm-09-03-0992: Immunohistochemical staining for matrix metalloproteinase-1 in the placenta of a patient with severe preeclampsia. Magnification, ×400.

Mentions: The expression of MMP-1 and TIMP-1 was mainly located in the cytomembrane and the cytoplasm of the placental trophoblasts. They were weakly dyed in the endothelial cells of the capillaries and the stromal fibroblasts, moderately dyed in the cytomembrane and the cytoplasm of the decidua, and weakly dyed in the cytoplasm of the spiral artery. The positive rates of expression of MMP-1 in the placenta of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 96.7, 77.8, 66.7 and 23.1%, respectively (Figs. 1–4, Table IV). There were significant differences in MMP-1 expression between each two groups (P<0.05). The positive rates of expression of MMP-1 in the decidua were 93.3, 77.8, 55.6 and 12.5%, respectively (Figs. 5–8, Table V). There were significant differences between each two groups (P<0.05).


Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia.

Deng CL, Ling ST, Liu XQ, Zhao YJ, Lv YF - Exp Ther Med (2015)

Immunohistochemical staining for matrix metalloproteinase-1 in the placenta of a patient with severe preeclampsia. Magnification, ×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316985&req=5

f4-etm-09-03-0992: Immunohistochemical staining for matrix metalloproteinase-1 in the placenta of a patient with severe preeclampsia. Magnification, ×400.
Mentions: The expression of MMP-1 and TIMP-1 was mainly located in the cytomembrane and the cytoplasm of the placental trophoblasts. They were weakly dyed in the endothelial cells of the capillaries and the stromal fibroblasts, moderately dyed in the cytomembrane and the cytoplasm of the decidua, and weakly dyed in the cytoplasm of the spiral artery. The positive rates of expression of MMP-1 in the placenta of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 96.7, 77.8, 66.7 and 23.1%, respectively (Figs. 1–4, Table IV). There were significant differences in MMP-1 expression between each two groups (P<0.05). The positive rates of expression of MMP-1 in the decidua were 93.3, 77.8, 55.6 and 12.5%, respectively (Figs. 5–8, Table V). There were significant differences between each two groups (P<0.05).

Bottom Line: The levels of MMP-1 in the umbilical serum of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 294.33±11.53, 247.78±20.32, 177.67±12.63 and 124.68±15.41 pg/ml, respectively, and there were significant differences between each two groups (P<0.05).However, no significant difference was identified between each two groups with regard to the levels of TIMP-1 in the umbilical cord and the positive rates in the placenta and decidua (P>0.05).Reduced MMP-1 levels in the umbilical serum, placenta and decidua were observed in women who developed preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynaecology and Obstetrics, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.

ABSTRACT

The aim of the present study was to explore the levels of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the maternal umbilical serum, placenta and decidua of patients with preeclampsia compared with those in normotensive pregnant females. A total of 73 pregnant females were recruited as the test subjects, including 43 inpatients with hypertensive disorders in pregnancy and 30 normal pregnant females as the control. The 43 inpatients with hypertensive disorders in pregnancy included 18 patients with gestational hypertension, nine with mild preeclampsia and 16 with severe preeclampsia. MMP-1 and TIMP-1 ELISA kits were used to determine the MMP-1 and TIMP-1 levels in the umbilical serum of the parturient following delivery. MMP-1 and TIMP-1 expressed in the placenta and decidua of the parturient following delivery were evaluated using immunohistochemistry. MMP-1 and TIMP-1 were mainly located in cytotrophoblasts and syncytiotrophoblasts in the placenta and decidua. The levels of MMP-1 in the umbilical serum of the normal, gestational hypertension, mild preeclampsia and severe preeclampsia groups were 294.33±11.53, 247.78±20.32, 177.67±12.63 and 124.68±15.41 pg/ml, respectively, and there were significant differences between each two groups (P<0.05). The positive expression rate of MMP-1 in the placenta and decidua of patients with hypertensive disorders in pregnancy was lower than that of the controls (P<0.01 and P<0.01, respectively). However, no significant difference was identified between each two groups with regard to the levels of TIMP-1 in the umbilical cord and the positive rates in the placenta and decidua (P>0.05). Reduced MMP-1 levels in the umbilical serum, placenta and decidua were observed in women who developed preeclampsia.

No MeSH data available.


Related in: MedlinePlus