Limits...
Effect of hydrogen sulfide on inflammatory cytokines in acute myocardial ischemia injury in rats.

Liu F, Liu GJ, Liu N, Zhang G, Zhang JX, Li LF - Exp Ther Med (2015)

Bottom Line: In the present study, sodium hydrosulfide (NaHS) was used as the H2S donor.In the ischemia + PPG group, the TNF-α, IL-1β and IL-6 levels in the serum were significantly increased, the expression of ICAM-1 mRNA was increased, although without a significant difference, and the expression of NF-κB was increased.The findings of the present study provide novel evidence for the dual effects of H2S on acute myocardial ischemia injury via the modulation of inflammatory factors.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China.

ABSTRACT

Hydrogen sulfide (H2S) is believed to be involved in numerous physiological and pathophysiological processes, and now it is recognized as the third endogenous signaling gasotransmitter, following nitric oxide and carbon monoxide; however, the effects of H2S on inflammatory factors in acute myocardial ischemia injury in rats have not been clarified. In the present study, sodium hydrosulfide (NaHS) was used as the H2S donor. Thirty-six male Sprague Dawley rats were randomly divided into five groups: Sham, ischemia, ischemia + low-dose (0.78 mg/kg) NaHS, ischemia + medium-dose (1.56 mg/kg) NaHS, ischemia + high-dose (3.12 mg/kg) NaHS and ischemia + propargylglycine (PPG) (30 mg/kg). The rats in each group were sacrificed 6 h after the surgery for sample collection. Compared with the ischemia group, the cardiac damage in the rats in the ischemia + NaHS groups was significantly reduced, particularly in the high-dose group; in the ischemia + PPG group, the myocardial injury was aggravated compared with that in the ischemia group. Compared with the ischemia group, the levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in the serum of rats in the ischemia + medium- and high-dose NaHS groups were significantly reduced, and the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) protein in the myocardial tissues of rats was significantly reduced. In the ischemia + PPG group, the TNF-α, IL-1β and IL-6 levels in the serum were significantly increased, the expression of ICAM-1 mRNA was increased, although without a significant difference, and the expression of NF-κB was increased. The findings of the present study provide novel evidence for the dual effects of H2S on acute myocardial ischemia injury via the modulation of inflammatory factors.

No MeSH data available.


Related in: MedlinePlus

Effect of hydrogen sulfide on the change in the expression of ICAM-1 mRNA in myocardial tissue in rats, as assessed using reverse transcription-polymerase chain reaction analysis: (a) Sham, (b) ischemia, (c) I + L NaHS, (d) I + M NaHS, (e) I + H NaHS, (F) I + PPG. Data are presented as the mean ± standard error of the mean (n=5). **P<0.01 vs. the sham group; #P<0.05 and ##P<0.01 vs. the ischemia group. Sham, rats underwent the surgical procedures but without the ischemic insult, followed by treatment with saline; Ischemia, rats underwent the surgical procedures and were then treated with saline; I + L NaHS, ischemic rats treated with 0.78 mg/kg NaHS; I + M NaHS, ischemic rats treated with 1.56 mg/kg NaHS; I + H-NaHS, ischemic rats treated with 3.12 mg/kg NaHS; I + PPG, ischemia rats treated with 30 mg/kg PPG; NaHS, sodium hydrosulfide; PPG, propargylglycine; ICAM-1, intercellular adhesion molecule-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4316979&req=5

f3-etm-09-03-1068: Effect of hydrogen sulfide on the change in the expression of ICAM-1 mRNA in myocardial tissue in rats, as assessed using reverse transcription-polymerase chain reaction analysis: (a) Sham, (b) ischemia, (c) I + L NaHS, (d) I + M NaHS, (e) I + H NaHS, (F) I + PPG. Data are presented as the mean ± standard error of the mean (n=5). **P<0.01 vs. the sham group; #P<0.05 and ##P<0.01 vs. the ischemia group. Sham, rats underwent the surgical procedures but without the ischemic insult, followed by treatment with saline; Ischemia, rats underwent the surgical procedures and were then treated with saline; I + L NaHS, ischemic rats treated with 0.78 mg/kg NaHS; I + M NaHS, ischemic rats treated with 1.56 mg/kg NaHS; I + H-NaHS, ischemic rats treated with 3.12 mg/kg NaHS; I + PPG, ischemia rats treated with 30 mg/kg PPG; NaHS, sodium hydrosulfide; PPG, propargylglycine; ICAM-1, intercellular adhesion molecule-1.

Mentions: In the ischemia group the ICAM-1 mRNA expression in the myocardial tissues of the rats was significantly increased compared with that in the sham surgery group (P<0.01). Compared with the ischemia group, the ICAM-1 mRNA expression in the myocardial tissues of the rats was reduced in the ischemia + low-, medium- and high-dose NaHS groups (P<0.05 or P<0.01); ICAM-1 mRNA expression was increased markedly, but not significantly, in the ischemia + PPG group (P>0.05) (Fig. 3 and Table II).


Effect of hydrogen sulfide on inflammatory cytokines in acute myocardial ischemia injury in rats.

Liu F, Liu GJ, Liu N, Zhang G, Zhang JX, Li LF - Exp Ther Med (2015)

Effect of hydrogen sulfide on the change in the expression of ICAM-1 mRNA in myocardial tissue in rats, as assessed using reverse transcription-polymerase chain reaction analysis: (a) Sham, (b) ischemia, (c) I + L NaHS, (d) I + M NaHS, (e) I + H NaHS, (F) I + PPG. Data are presented as the mean ± standard error of the mean (n=5). **P<0.01 vs. the sham group; #P<0.05 and ##P<0.01 vs. the ischemia group. Sham, rats underwent the surgical procedures but without the ischemic insult, followed by treatment with saline; Ischemia, rats underwent the surgical procedures and were then treated with saline; I + L NaHS, ischemic rats treated with 0.78 mg/kg NaHS; I + M NaHS, ischemic rats treated with 1.56 mg/kg NaHS; I + H-NaHS, ischemic rats treated with 3.12 mg/kg NaHS; I + PPG, ischemia rats treated with 30 mg/kg PPG; NaHS, sodium hydrosulfide; PPG, propargylglycine; ICAM-1, intercellular adhesion molecule-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316979&req=5

f3-etm-09-03-1068: Effect of hydrogen sulfide on the change in the expression of ICAM-1 mRNA in myocardial tissue in rats, as assessed using reverse transcription-polymerase chain reaction analysis: (a) Sham, (b) ischemia, (c) I + L NaHS, (d) I + M NaHS, (e) I + H NaHS, (F) I + PPG. Data are presented as the mean ± standard error of the mean (n=5). **P<0.01 vs. the sham group; #P<0.05 and ##P<0.01 vs. the ischemia group. Sham, rats underwent the surgical procedures but without the ischemic insult, followed by treatment with saline; Ischemia, rats underwent the surgical procedures and were then treated with saline; I + L NaHS, ischemic rats treated with 0.78 mg/kg NaHS; I + M NaHS, ischemic rats treated with 1.56 mg/kg NaHS; I + H-NaHS, ischemic rats treated with 3.12 mg/kg NaHS; I + PPG, ischemia rats treated with 30 mg/kg PPG; NaHS, sodium hydrosulfide; PPG, propargylglycine; ICAM-1, intercellular adhesion molecule-1.
Mentions: In the ischemia group the ICAM-1 mRNA expression in the myocardial tissues of the rats was significantly increased compared with that in the sham surgery group (P<0.01). Compared with the ischemia group, the ICAM-1 mRNA expression in the myocardial tissues of the rats was reduced in the ischemia + low-, medium- and high-dose NaHS groups (P<0.05 or P<0.01); ICAM-1 mRNA expression was increased markedly, but not significantly, in the ischemia + PPG group (P>0.05) (Fig. 3 and Table II).

Bottom Line: In the present study, sodium hydrosulfide (NaHS) was used as the H2S donor.In the ischemia + PPG group, the TNF-α, IL-1β and IL-6 levels in the serum were significantly increased, the expression of ICAM-1 mRNA was increased, although without a significant difference, and the expression of NF-κB was increased.The findings of the present study provide novel evidence for the dual effects of H2S on acute myocardial ischemia injury via the modulation of inflammatory factors.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China.

ABSTRACT

Hydrogen sulfide (H2S) is believed to be involved in numerous physiological and pathophysiological processes, and now it is recognized as the third endogenous signaling gasotransmitter, following nitric oxide and carbon monoxide; however, the effects of H2S on inflammatory factors in acute myocardial ischemia injury in rats have not been clarified. In the present study, sodium hydrosulfide (NaHS) was used as the H2S donor. Thirty-six male Sprague Dawley rats were randomly divided into five groups: Sham, ischemia, ischemia + low-dose (0.78 mg/kg) NaHS, ischemia + medium-dose (1.56 mg/kg) NaHS, ischemia + high-dose (3.12 mg/kg) NaHS and ischemia + propargylglycine (PPG) (30 mg/kg). The rats in each group were sacrificed 6 h after the surgery for sample collection. Compared with the ischemia group, the cardiac damage in the rats in the ischemia + NaHS groups was significantly reduced, particularly in the high-dose group; in the ischemia + PPG group, the myocardial injury was aggravated compared with that in the ischemia group. Compared with the ischemia group, the levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in the serum of rats in the ischemia + medium- and high-dose NaHS groups were significantly reduced, and the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) protein in the myocardial tissues of rats was significantly reduced. In the ischemia + PPG group, the TNF-α, IL-1β and IL-6 levels in the serum were significantly increased, the expression of ICAM-1 mRNA was increased, although without a significant difference, and the expression of NF-κB was increased. The findings of the present study provide novel evidence for the dual effects of H2S on acute myocardial ischemia injury via the modulation of inflammatory factors.

No MeSH data available.


Related in: MedlinePlus