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Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression.

Li X, Chen J, Liang W, Li H, Liu F, Weng X, Lin P, Chen W, Zheng C, Xu H, Liu X, Ye H - Exp Ther Med (2015)

Bottom Line: It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner.To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells.The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT

Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription-polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA.

No MeSH data available.


Related in: MedlinePlus

BZD affects the protein levels of the cell cycle regulators in chondrocytes. (A) Western blot analysis showing the protein expression of cell cycle regulators in chondrocytes treated with or without BZD. (B-E) Protein levels of (B) cyclin D1, (C) CDK4, (D) CDK6 and (E) p21 in chondrocytes treated with or without BZD. β-actin was used as the reference protein for the quantification analysis. Data are presented as the mean ± standard deviation, shown as vertical bars. *P<0.05 and **P<0.01, compared with untreated cells. BZD, Bushen Zhuangjin Decoction; CDK, cyclin-dependent kinase.
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f5-etm-09-03-0839: BZD affects the protein levels of the cell cycle regulators in chondrocytes. (A) Western blot analysis showing the protein expression of cell cycle regulators in chondrocytes treated with or without BZD. (B-E) Protein levels of (B) cyclin D1, (C) CDK4, (D) CDK6 and (E) p21 in chondrocytes treated with or without BZD. β-actin was used as the reference protein for the quantification analysis. Data are presented as the mean ± standard deviation, shown as vertical bars. *P<0.05 and **P<0.01, compared with untreated cells. BZD, Bushen Zhuangjin Decoction; CDK, cyclin-dependent kinase.

Mentions: To gain an insight into the effect of BZD on the cell cycle of chondrocytes, the mRNA and protein expression of cyclin D1, CDK4, CDK6 and p21 was analyzed using RT-PCR and western blotting. Compared with the untreated cells, the mRNA expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated (P<0.01 or P<0.05), while the mRNA expression of p21 was significantly downregulated (P<0.05) (Fig. 4). The protein levels of cyclin D1, CDK4, CDK6 and p21 were similar to their respective mRNA expression (Fig. 5).


Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression.

Li X, Chen J, Liang W, Li H, Liu F, Weng X, Lin P, Chen W, Zheng C, Xu H, Liu X, Ye H - Exp Ther Med (2015)

BZD affects the protein levels of the cell cycle regulators in chondrocytes. (A) Western blot analysis showing the protein expression of cell cycle regulators in chondrocytes treated with or without BZD. (B-E) Protein levels of (B) cyclin D1, (C) CDK4, (D) CDK6 and (E) p21 in chondrocytes treated with or without BZD. β-actin was used as the reference protein for the quantification analysis. Data are presented as the mean ± standard deviation, shown as vertical bars. *P<0.05 and **P<0.01, compared with untreated cells. BZD, Bushen Zhuangjin Decoction; CDK, cyclin-dependent kinase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316974&req=5

f5-etm-09-03-0839: BZD affects the protein levels of the cell cycle regulators in chondrocytes. (A) Western blot analysis showing the protein expression of cell cycle regulators in chondrocytes treated with or without BZD. (B-E) Protein levels of (B) cyclin D1, (C) CDK4, (D) CDK6 and (E) p21 in chondrocytes treated with or without BZD. β-actin was used as the reference protein for the quantification analysis. Data are presented as the mean ± standard deviation, shown as vertical bars. *P<0.05 and **P<0.01, compared with untreated cells. BZD, Bushen Zhuangjin Decoction; CDK, cyclin-dependent kinase.
Mentions: To gain an insight into the effect of BZD on the cell cycle of chondrocytes, the mRNA and protein expression of cyclin D1, CDK4, CDK6 and p21 was analyzed using RT-PCR and western blotting. Compared with the untreated cells, the mRNA expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated (P<0.01 or P<0.05), while the mRNA expression of p21 was significantly downregulated (P<0.05) (Fig. 4). The protein levels of cyclin D1, CDK4, CDK6 and p21 were similar to their respective mRNA expression (Fig. 5).

Bottom Line: It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner.To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells.The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT

Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription-polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA.

No MeSH data available.


Related in: MedlinePlus