Limits...
Preparation of novel (-)-gossypol nanoparticles and the effect on growth inhibition in human prostate cancer PC-3 cells in vitro.

Jin CL, Chen ML, Wang Y, Kang XC, Han GY, Xu SL - Exp Ther Med (2015)

Bottom Line: The aim of the present study was to investigate the antitumor effects and possible mechanism of (-)-gossypol nanoparticles, loaded with vv polyethylene glycol-maleimide (mPEG-Mal), in vitro.The growth inhibition activity of the loaded (-)-gossypol nanoparticles was found to be dose- and time-dependent, and similar to the activity of free (-)-gossypol.The nanoparticles induced apoptotic morphological changes on the PC-3 cells, downregulating the mRNA expression level of Bcl-2 and upregulating the mRNA expression level of Bak.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.

ABSTRACT

The aim of the present study was to investigate the antitumor effects and possible mechanism of (-)-gossypol nanoparticles, loaded with vv polyethylene glycol-maleimide (mPEG-Mal), in vitro. Emulsification-volatilization was used to prepare the loaded (-)-gossypol nanoparticles. The toxicity of blank nanoparticles on human prostate cancer PC-3 cells and human prostate RWPE-1 cells was measured. The antitumor effects of the nanoparticles on PC-3 cells were evaluated by an MTT assay, acridine orange staining and transmission electron microscopy in vitro, and the results were compared with those of free (-)-gossypol. In addition, the mRNA expression levels of Bcl-2 and Bak were measured using semi-quantitative reverse transcription polymerase chain reaction. The growth inhibition activity of the loaded (-)-gossypol nanoparticles was found to be dose- and time-dependent, and similar to the activity of free (-)-gossypol. The nanoparticles induced apoptotic morphological changes on the PC-3 cells, downregulating the mRNA expression level of Bcl-2 and upregulating the mRNA expression level of Bak. Blank nanoparticles exhibited no evident toxicity on PC-3 and RWPE-1 cells at a high dose. Therefore, the mPEG-Mal loaded (-)-gossypol nanoparticles demonstrated a favorable antitumor activity and no toxicity. The nanoparticles were able to induce the apoptosis of prostate cancer cells; thus, may be a potential antitumor nanodrug.

No MeSH data available.


Related in: MedlinePlus

Effect of (-)-gossypol nanoparticles and free (-)-gossypol at different concentrations on the growth of PC-3 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4316968&req=5

f2-etm-09-03-0675: Effect of (-)-gossypol nanoparticles and free (-)-gossypol at different concentrations on the growth of PC-3 cells.

Mentions: When the (-)-gossypol nanoparticles and free (-)-gossypol reached a concentration of 10.0 μg/ml, they demonstrated evident antitumor activity against prostate cancer PC-3 cells in vitro (Fig. 1). As shown in Fig. 1, the inhibition effects of (-)-gossypol nanoparticles and free (-)-gossypol on the proliferation of PC-3 cells increased with time. In addition, following culture for 72 h, the inhibition effects of (-)-gossypol nanoparticles and free (-)-gossypol on the proliferation of PC-3 cells increased with increasing concentration (Fig. 2). At the various time points, the IC50 of the (-)-gossypol nanoparticles was slightly higher compared with the free (-)-gossypol; however, no statistically significant difference was observed (P>0.05).


Preparation of novel (-)-gossypol nanoparticles and the effect on growth inhibition in human prostate cancer PC-3 cells in vitro.

Jin CL, Chen ML, Wang Y, Kang XC, Han GY, Xu SL - Exp Ther Med (2015)

Effect of (-)-gossypol nanoparticles and free (-)-gossypol at different concentrations on the growth of PC-3 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316968&req=5

f2-etm-09-03-0675: Effect of (-)-gossypol nanoparticles and free (-)-gossypol at different concentrations on the growth of PC-3 cells.
Mentions: When the (-)-gossypol nanoparticles and free (-)-gossypol reached a concentration of 10.0 μg/ml, they demonstrated evident antitumor activity against prostate cancer PC-3 cells in vitro (Fig. 1). As shown in Fig. 1, the inhibition effects of (-)-gossypol nanoparticles and free (-)-gossypol on the proliferation of PC-3 cells increased with time. In addition, following culture for 72 h, the inhibition effects of (-)-gossypol nanoparticles and free (-)-gossypol on the proliferation of PC-3 cells increased with increasing concentration (Fig. 2). At the various time points, the IC50 of the (-)-gossypol nanoparticles was slightly higher compared with the free (-)-gossypol; however, no statistically significant difference was observed (P>0.05).

Bottom Line: The aim of the present study was to investigate the antitumor effects and possible mechanism of (-)-gossypol nanoparticles, loaded with vv polyethylene glycol-maleimide (mPEG-Mal), in vitro.The growth inhibition activity of the loaded (-)-gossypol nanoparticles was found to be dose- and time-dependent, and similar to the activity of free (-)-gossypol.The nanoparticles induced apoptotic morphological changes on the PC-3 cells, downregulating the mRNA expression level of Bcl-2 and upregulating the mRNA expression level of Bak.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.

ABSTRACT

The aim of the present study was to investigate the antitumor effects and possible mechanism of (-)-gossypol nanoparticles, loaded with vv polyethylene glycol-maleimide (mPEG-Mal), in vitro. Emulsification-volatilization was used to prepare the loaded (-)-gossypol nanoparticles. The toxicity of blank nanoparticles on human prostate cancer PC-3 cells and human prostate RWPE-1 cells was measured. The antitumor effects of the nanoparticles on PC-3 cells were evaluated by an MTT assay, acridine orange staining and transmission electron microscopy in vitro, and the results were compared with those of free (-)-gossypol. In addition, the mRNA expression levels of Bcl-2 and Bak were measured using semi-quantitative reverse transcription polymerase chain reaction. The growth inhibition activity of the loaded (-)-gossypol nanoparticles was found to be dose- and time-dependent, and similar to the activity of free (-)-gossypol. The nanoparticles induced apoptotic morphological changes on the PC-3 cells, downregulating the mRNA expression level of Bcl-2 and upregulating the mRNA expression level of Bak. Blank nanoparticles exhibited no evident toxicity on PC-3 and RWPE-1 cells at a high dose. Therefore, the mPEG-Mal loaded (-)-gossypol nanoparticles demonstrated a favorable antitumor activity and no toxicity. The nanoparticles were able to induce the apoptosis of prostate cancer cells; thus, may be a potential antitumor nanodrug.

No MeSH data available.


Related in: MedlinePlus