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Green tea extract therapy diminishes hepatic fibrosis mediated by dual exposure to carbon tetrachloride and ethanol: A histopathological study.

Safer AM, Afzal M, Hanafy N, Mousa S - Exp Ther Med (2014)

Bottom Line: The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status.The autopsy results also showed that the animal livers returned to the normal shape and color.GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City 13060, Kuwait.

ABSTRACT

The aim of the present study was to investigate the hepatoprotective effect of green tea extract (GTE) against the hepatic fibrosis induced by carbon tetrachloride (CCl4), ethanol, and dual exposure to CCl4 plus ethanol in rats. In particular, an investigation of the three-dimensional architecture was conducted using scanning electron microscopy. Various techniques revealed that hepatic fibrosis with intermingled fibers was located between cells in the CCl4, ethanol and combined CCl4 plus ethanol groups. The hepatic fibrosis differed among the ethanol, CCl4 and CCl4 plus ethanol groups in terms of the type, thickness and distribution of fibers. The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status. The rats were also found to regain normal body weight and fur color, which had earlier been discolored due to weight loss. The autopsy results also showed that the animal livers returned to the normal shape and color. GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal. GTE presents a safe therapeutic strategy for hepatic fibrosis.

No MeSH data available.


Related in: MedlinePlus

AST and ALT levels. (A) AST levels and (B) ALT levels in rats treated with CCl4, ethanol, and CCl4 plus ethanol in the presence and absence of GTE. Groups: GI, normal control; GII, CCl4 plus ethanol; GIII, CCl4 plus ethanol plus GTE; GIV, ethanol; GV, ethanol plus GTE; GVI, CCl4; GVII, CCl4 plus GTE. AST, aspartate aminotransferase; ALT, alanine aminotransferase; SD, standard deviation; CCl4, carbon tetrachloride; GTE, green tea extract.
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f1-etm-09-03-0787: AST and ALT levels. (A) AST levels and (B) ALT levels in rats treated with CCl4, ethanol, and CCl4 plus ethanol in the presence and absence of GTE. Groups: GI, normal control; GII, CCl4 plus ethanol; GIII, CCl4 plus ethanol plus GTE; GIV, ethanol; GV, ethanol plus GTE; GVI, CCl4; GVII, CCl4 plus GTE. AST, aspartate aminotransferase; ALT, alanine aminotransferase; SD, standard deviation; CCl4, carbon tetrachloride; GTE, green tea extract.

Mentions: Rats treated with GTE showed results similar to those of the control groups with normal AST and ALT levels (Fig. 1; Table I). Serum AST and ALT levels were significantly elevated in rats treated with CCl4 (GVI), ethanol (GIV), and CCl4 plus ethanol only (GII), indicating severe hepatic damage. The CCl4 plus GTE (GVII), ethanol plus GTE (GV), and CCl4 plus ethanol plus GTE (GIII) groups showed significant reductions in the levels of these two enzymes compared with those in their respective model control groups.


Green tea extract therapy diminishes hepatic fibrosis mediated by dual exposure to carbon tetrachloride and ethanol: A histopathological study.

Safer AM, Afzal M, Hanafy N, Mousa S - Exp Ther Med (2014)

AST and ALT levels. (A) AST levels and (B) ALT levels in rats treated with CCl4, ethanol, and CCl4 plus ethanol in the presence and absence of GTE. Groups: GI, normal control; GII, CCl4 plus ethanol; GIII, CCl4 plus ethanol plus GTE; GIV, ethanol; GV, ethanol plus GTE; GVI, CCl4; GVII, CCl4 plus GTE. AST, aspartate aminotransferase; ALT, alanine aminotransferase; SD, standard deviation; CCl4, carbon tetrachloride; GTE, green tea extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316931&req=5

f1-etm-09-03-0787: AST and ALT levels. (A) AST levels and (B) ALT levels in rats treated with CCl4, ethanol, and CCl4 plus ethanol in the presence and absence of GTE. Groups: GI, normal control; GII, CCl4 plus ethanol; GIII, CCl4 plus ethanol plus GTE; GIV, ethanol; GV, ethanol plus GTE; GVI, CCl4; GVII, CCl4 plus GTE. AST, aspartate aminotransferase; ALT, alanine aminotransferase; SD, standard deviation; CCl4, carbon tetrachloride; GTE, green tea extract.
Mentions: Rats treated with GTE showed results similar to those of the control groups with normal AST and ALT levels (Fig. 1; Table I). Serum AST and ALT levels were significantly elevated in rats treated with CCl4 (GVI), ethanol (GIV), and CCl4 plus ethanol only (GII), indicating severe hepatic damage. The CCl4 plus GTE (GVII), ethanol plus GTE (GV), and CCl4 plus ethanol plus GTE (GIII) groups showed significant reductions in the levels of these two enzymes compared with those in their respective model control groups.

Bottom Line: The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status.The autopsy results also showed that the animal livers returned to the normal shape and color.GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City 13060, Kuwait.

ABSTRACT

The aim of the present study was to investigate the hepatoprotective effect of green tea extract (GTE) against the hepatic fibrosis induced by carbon tetrachloride (CCl4), ethanol, and dual exposure to CCl4 plus ethanol in rats. In particular, an investigation of the three-dimensional architecture was conducted using scanning electron microscopy. Various techniques revealed that hepatic fibrosis with intermingled fibers was located between cells in the CCl4, ethanol and combined CCl4 plus ethanol groups. The hepatic fibrosis differed among the ethanol, CCl4 and CCl4 plus ethanol groups in terms of the type, thickness and distribution of fibers. The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status. The rats were also found to regain normal body weight and fur color, which had earlier been discolored due to weight loss. The autopsy results also showed that the animal livers returned to the normal shape and color. GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal. GTE presents a safe therapeutic strategy for hepatic fibrosis.

No MeSH data available.


Related in: MedlinePlus