Limits...
Expression and significance of NELIN and SM22α in varicose vein tissue.

Chen S, Qin S, Wang M, Zhang S - Exp Ther Med (2015)

Bottom Line: RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01).In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05).The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Dezhou Municipal Hospital, Dezhou, Shandong 253000, P.R. China.

ABSTRACT

The aim of the present study was to investigate the expression of NELIN and SM22α in lower extremity varicose vein tissue, and their association with varicose veins. Tissue samples were collected from 18 patients with lower extremity varicose veins for the experimental group, while normal great saphenous vein tissue was reserved during coronary artery bypass surgery from 14 patients for the controls. Reverse transcription polymerase chain reaction (RT-PCR) analysis was applied to detect the mRNA expression levels of NELIN and SM22α, while immunohistochemical techniques were used to detect the protein expression levels in the normal and abnormal veins. RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01). In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05). The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins. Therefore, NELIN and SM22α were demonstrated to play a key role in the development of varicosity.

No MeSH data available.


Related in: MedlinePlus

IOD of NELIN protein expression staining. Vascular endothelial growth factor expression was significantly decreased in the experimental group compared with the control group (P<0.05). Data are presented as the mean ± standard deviation. IOD, integral optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4316901&req=5

f5-etm-09-03-0845: IOD of NELIN protein expression staining. Vascular endothelial growth factor expression was significantly decreased in the experimental group compared with the control group (P<0.05). Data are presented as the mean ± standard deviation. IOD, integral optical density.

Mentions: Positive staining of NELIN (Fig. 3) and SM22α (Fig. 4) was observed in the cytoplasm of the cells. Immunohistochemical staining revealed a significant decrease in the expression levels of NELIN and SM22α in the experimental group when compared with the control (P<0.05). According to the statistical analysis, the IOD value of the NELIN protein staining was significantly reduced in the experimental group (7422.56±2423) when compared with the control group (9785.85±3005.85; P<0.05; Fig. 5). Similarly, the IOD value of SM22α staining was significantly reduced in the experimental group (10054.79±3584.07) when compared with the control group (16226.05±3378.16; P<0.05; Fig. 6).


Expression and significance of NELIN and SM22α in varicose vein tissue.

Chen S, Qin S, Wang M, Zhang S - Exp Ther Med (2015)

IOD of NELIN protein expression staining. Vascular endothelial growth factor expression was significantly decreased in the experimental group compared with the control group (P<0.05). Data are presented as the mean ± standard deviation. IOD, integral optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316901&req=5

f5-etm-09-03-0845: IOD of NELIN protein expression staining. Vascular endothelial growth factor expression was significantly decreased in the experimental group compared with the control group (P<0.05). Data are presented as the mean ± standard deviation. IOD, integral optical density.
Mentions: Positive staining of NELIN (Fig. 3) and SM22α (Fig. 4) was observed in the cytoplasm of the cells. Immunohistochemical staining revealed a significant decrease in the expression levels of NELIN and SM22α in the experimental group when compared with the control (P<0.05). According to the statistical analysis, the IOD value of the NELIN protein staining was significantly reduced in the experimental group (7422.56±2423) when compared with the control group (9785.85±3005.85; P<0.05; Fig. 5). Similarly, the IOD value of SM22α staining was significantly reduced in the experimental group (10054.79±3584.07) when compared with the control group (16226.05±3378.16; P<0.05; Fig. 6).

Bottom Line: RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01).In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05).The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Dezhou Municipal Hospital, Dezhou, Shandong 253000, P.R. China.

ABSTRACT

The aim of the present study was to investigate the expression of NELIN and SM22α in lower extremity varicose vein tissue, and their association with varicose veins. Tissue samples were collected from 18 patients with lower extremity varicose veins for the experimental group, while normal great saphenous vein tissue was reserved during coronary artery bypass surgery from 14 patients for the controls. Reverse transcription polymerase chain reaction (RT-PCR) analysis was applied to detect the mRNA expression levels of NELIN and SM22α, while immunohistochemical techniques were used to detect the protein expression levels in the normal and abnormal veins. RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01). In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05). The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins. Therefore, NELIN and SM22α were demonstrated to play a key role in the development of varicosity.

No MeSH data available.


Related in: MedlinePlus