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Molecular Survey of Toxoplasma gondii in Sheep, Cattle and Meat Products in Chaharmahal va Bakhtiari Province, Southwest of Iran.

Azizi H, Shiran B, Boroujeni AB, Jafari M - Iran J Parasitol (2014)

Bottom Line: Toxoplasmosis is a worldwide spread disease.The tongue, brain, femur muscle and liver of 50 sheep and 70 cattle as well as 50 samples of meat products were selected and collected to perform molecular survey using Nested-PCR method.Due to the high rate of this parasitic infection among the slaughtered animals as well as meat products in this region, the use of infected material can be one of the main risk factors of transmission of the parasite to humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Toxoplasmosis is a worldwide spread disease. The present study examined the prevalence of Toxoplasma gondii infection among animals of edible meat (cattle and sheep) in Chaharmahal va Bakhtiari Province (Southwest of Iran) in 2012. Furthermore, we attempted for the first time to identify this parasite from the meat products in the province.

Methods: The tongue, brain, femur muscle and liver of 50 sheep and 70 cattle as well as 50 samples of meat products were selected and collected to perform molecular survey using Nested-PCR method.

Results: Of the studied sheep, 38% were infected. The infection rate in the age groups under 1 year, 1-2 years, and more than 2 years was 25%, 35.29% and 52.94%, respectively. The infection rate in femur muscle, brain, liver and tongue was 28%, 32%, 30% and 16%, respectively. Of the studied cattle, 8.57% were infected. The infection rate in the age groups 1-2 years, 2-4 years, and more than 4 years was 3.7%, 9.09% and 14.28%, respectively. Sheep was infected 6 times more than cattle (OR = 6.53 CI = 2.374-18.005).The infection rate among samples of meat products was 12% (6 samples out of 50 samples).

Conclusion: Due to the high rate of this parasitic infection among the slaughtered animals as well as meat products in this region, the use of infected material can be one of the main risk factors of transmission of the parasite to humans.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis of Nested-PCR products of tissue samples of sheep, Lane M represents 100-bp DNA ladder. Lane P.C is for positive control. Lane N.C for negative control. Lane 3 and 9 are positive samples. (F: femur muscle, B: brain, T: tongue, L: liver)
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Figure 1: Agarose gel electrophoresis of Nested-PCR products of tissue samples of sheep, Lane M represents 100-bp DNA ladder. Lane P.C is for positive control. Lane N.C for negative control. Lane 3 and 9 are positive samples. (F: femur muscle, B: brain, T: tongue, L: liver)

Mentions: The most significant correlation was observed between tongue and femur muscle infection (P = 0.001, R = 0.556), and the brain and tongue infection (P = 0.001, R =.47), respectively. Sheep was infected 6 times more than cattle (OR = 6.53 CI = 2.374-18.005). The infection rate among samples of meat products was 12%. The results of PCR amplification and Nested-PCR products of tissue samples are shown in Fig. 1-3.


Molecular Survey of Toxoplasma gondii in Sheep, Cattle and Meat Products in Chaharmahal va Bakhtiari Province, Southwest of Iran.

Azizi H, Shiran B, Boroujeni AB, Jafari M - Iran J Parasitol (2014)

Agarose gel electrophoresis of Nested-PCR products of tissue samples of sheep, Lane M represents 100-bp DNA ladder. Lane P.C is for positive control. Lane N.C for negative control. Lane 3 and 9 are positive samples. (F: femur muscle, B: brain, T: tongue, L: liver)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4316576&req=5

Figure 1: Agarose gel electrophoresis of Nested-PCR products of tissue samples of sheep, Lane M represents 100-bp DNA ladder. Lane P.C is for positive control. Lane N.C for negative control. Lane 3 and 9 are positive samples. (F: femur muscle, B: brain, T: tongue, L: liver)
Mentions: The most significant correlation was observed between tongue and femur muscle infection (P = 0.001, R = 0.556), and the brain and tongue infection (P = 0.001, R =.47), respectively. Sheep was infected 6 times more than cattle (OR = 6.53 CI = 2.374-18.005). The infection rate among samples of meat products was 12%. The results of PCR amplification and Nested-PCR products of tissue samples are shown in Fig. 1-3.

Bottom Line: Toxoplasmosis is a worldwide spread disease.The tongue, brain, femur muscle and liver of 50 sheep and 70 cattle as well as 50 samples of meat products were selected and collected to perform molecular survey using Nested-PCR method.Due to the high rate of this parasitic infection among the slaughtered animals as well as meat products in this region, the use of infected material can be one of the main risk factors of transmission of the parasite to humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Toxoplasmosis is a worldwide spread disease. The present study examined the prevalence of Toxoplasma gondii infection among animals of edible meat (cattle and sheep) in Chaharmahal va Bakhtiari Province (Southwest of Iran) in 2012. Furthermore, we attempted for the first time to identify this parasite from the meat products in the province.

Methods: The tongue, brain, femur muscle and liver of 50 sheep and 70 cattle as well as 50 samples of meat products were selected and collected to perform molecular survey using Nested-PCR method.

Results: Of the studied sheep, 38% were infected. The infection rate in the age groups under 1 year, 1-2 years, and more than 2 years was 25%, 35.29% and 52.94%, respectively. The infection rate in femur muscle, brain, liver and tongue was 28%, 32%, 30% and 16%, respectively. Of the studied cattle, 8.57% were infected. The infection rate in the age groups 1-2 years, 2-4 years, and more than 4 years was 3.7%, 9.09% and 14.28%, respectively. Sheep was infected 6 times more than cattle (OR = 6.53 CI = 2.374-18.005).The infection rate among samples of meat products was 12% (6 samples out of 50 samples).

Conclusion: Due to the high rate of this parasitic infection among the slaughtered animals as well as meat products in this region, the use of infected material can be one of the main risk factors of transmission of the parasite to humans.

No MeSH data available.


Related in: MedlinePlus