Limits...
Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice.

Mohammadzadeh Hajipirloo H, Bozorgomd A, Shahabi S, Hazrati Tappeh K, Karamati SA - Iran J Parasitol (2014)

Bottom Line: The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major.There was no significant difference in survival following challenge among groups.Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

ABSTRACT

Background: Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model.

Methods: BALB/c mice were immunized three times either autoclaved L. major promastigotes' antigens alone or in combination with the adjuvant alum, naltrexone or the alum-naltrexone mixture. Both humoral and cellular immune responses were assessed two weeks after the last immunization and compared with control mice.

Results: The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major. There was no significant difference in survival following challenge among groups.

Conclusion: Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.

No MeSH data available.


Related in: MedlinePlus

DTH response to parasite antigens in BALB/c mice immunized with different adjuvantsThree weeks after last immunization 1×10 7 freeze-thawed L. major promastigotes and PBS in a final volume of 50 μl was injected in left and right footpad of mice, respectively. The difference in footpad swelling was measured at 72 hours using a metric caliper. Result showed that there was a significant difference (P< 0. 01) in induction of DTH response Al– NTX –Vac as compared to the control group of mice. No significant difference was seen between other groups
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4316561&req=5

Figure 3: DTH response to parasite antigens in BALB/c mice immunized with different adjuvantsThree weeks after last immunization 1×10 7 freeze-thawed L. major promastigotes and PBS in a final volume of 50 μl was injected in left and right footpad of mice, respectively. The difference in footpad swelling was measured at 72 hours using a metric caliper. Result showed that there was a significant difference (P< 0. 01) in induction of DTH response Al– NTX –Vac as compared to the control group of mice. No significant difference was seen between other groups

Mentions: To assess cell-mediated responses to parasite antigens in vivo, the DTH reaction was measured 72 h post infection. The results of DTH showed that different groups of immunized mice induced a stronger DTH response than PBS control group (Fig 3); however, the difference was not statistically significant except for the Al– NTX – Vac group (P< 0. 01).


Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice.

Mohammadzadeh Hajipirloo H, Bozorgomd A, Shahabi S, Hazrati Tappeh K, Karamati SA - Iran J Parasitol (2014)

DTH response to parasite antigens in BALB/c mice immunized with different adjuvantsThree weeks after last immunization 1×10 7 freeze-thawed L. major promastigotes and PBS in a final volume of 50 μl was injected in left and right footpad of mice, respectively. The difference in footpad swelling was measured at 72 hours using a metric caliper. Result showed that there was a significant difference (P< 0. 01) in induction of DTH response Al– NTX –Vac as compared to the control group of mice. No significant difference was seen between other groups
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4316561&req=5

Figure 3: DTH response to parasite antigens in BALB/c mice immunized with different adjuvantsThree weeks after last immunization 1×10 7 freeze-thawed L. major promastigotes and PBS in a final volume of 50 μl was injected in left and right footpad of mice, respectively. The difference in footpad swelling was measured at 72 hours using a metric caliper. Result showed that there was a significant difference (P< 0. 01) in induction of DTH response Al– NTX –Vac as compared to the control group of mice. No significant difference was seen between other groups
Mentions: To assess cell-mediated responses to parasite antigens in vivo, the DTH reaction was measured 72 h post infection. The results of DTH showed that different groups of immunized mice induced a stronger DTH response than PBS control group (Fig 3); however, the difference was not statistically significant except for the Al– NTX – Vac group (P< 0. 01).

Bottom Line: The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major.There was no significant difference in survival following challenge among groups.Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

ABSTRACT

Background: Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model.

Methods: BALB/c mice were immunized three times either autoclaved L. major promastigotes' antigens alone or in combination with the adjuvant alum, naltrexone or the alum-naltrexone mixture. Both humoral and cellular immune responses were assessed two weeks after the last immunization and compared with control mice.

Results: The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major. There was no significant difference in survival following challenge among groups.

Conclusion: Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.

No MeSH data available.


Related in: MedlinePlus