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A more consistent intraluminal rhesus monkey model of ischemic stroke.

Zhao B, Shang G, Chen J, Geng X, Ye X, Xu G, Wang J, Zheng J, Li H, Akbary F, Li S, Lu J, Ling F, Ji X - Neural Regen Res (2014)

Bottom Line: Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients.However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies.The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

ABSTRACT
Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients. However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies. In this study, eight healthy adult rhesus monkeys were randomized into two groups with four monkeys in each group: middle cerebral artery occlusion at origin segment (M1) and middle cerebral artery occlusion at M2 segment. The blood flow in the middle cerebral artery was blocked completely for 2 hours using the endovascular microcoil placement technique (1 mm × 10 cm) (undetachable), to establish a model of cerebral ischemia. The microcoil was withdrawn and the middle cerebral artery blood flow was restored. A reversible middle cerebral artery occlusion model was identified by hematoxylin-eosin staining, digital subtraction angiography, magnetic resonance angiography, magnetic resonance imaging, and neurological evaluation. The results showed that the middle cerebral artery occlusion model was successfully established in eight adult healthy rhesus monkeys, and ischemic lesions were apparent in the brain tissue of rhesus monkeys at 24 hours after occlusion. The rhesus monkeys had symptoms of neurological deficits. Compared with the M1 occlusion group, the M2 occlusion group had lower infarction volume and higher neurological scores. These experimental findings indicate that reversible middle cerebral artery occlusion can be produced with the endovascular microcoil technique in rhesus monkeys. The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research.

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Correlation analysis of infarct volume and neurological evaluation scores in the two groups.Pearson parametric correlation analysis showed that ischemic damage measured by MRI was significantly negatively correlated with neuro-logical evaluations (stroke scores). “■”: M1 occlusion group; “▲”: M2 occlusion group; “—” : the linear regression line; “::::::::”: the 95% confidence band of the best-fit line.
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Figure 4: Correlation analysis of infarct volume and neurological evaluation scores in the two groups.Pearson parametric correlation analysis showed that ischemic damage measured by MRI was significantly negatively correlated with neuro-logical evaluations (stroke scores). “■”: M1 occlusion group; “▲”: M2 occlusion group; “—” : the linear regression line; “::::::::”: the 95% confidence band of the best-fit line.

Mentions: Both the MRI images and histological examination confirmed that the M1 occlusion group had a larger infarction volume than did the M2 occlusion group, and was associated with significantly lower neurological evaluation scores. Statistical analysis of infarction volume and neurological evaluation score showed a significant difference between the two groups (Figure 3A, B). The neurological evaluation scores after embolization were significantly negatively correlated with infarct volume (r = −0.8649, P = 0.0056) (Figure 4).


A more consistent intraluminal rhesus monkey model of ischemic stroke.

Zhao B, Shang G, Chen J, Geng X, Ye X, Xu G, Wang J, Zheng J, Li H, Akbary F, Li S, Lu J, Ling F, Ji X - Neural Regen Res (2014)

Correlation analysis of infarct volume and neurological evaluation scores in the two groups.Pearson parametric correlation analysis showed that ischemic damage measured by MRI was significantly negatively correlated with neuro-logical evaluations (stroke scores). “■”: M1 occlusion group; “▲”: M2 occlusion group; “—” : the linear regression line; “::::::::”: the 95% confidence band of the best-fit line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316474&req=5

Figure 4: Correlation analysis of infarct volume and neurological evaluation scores in the two groups.Pearson parametric correlation analysis showed that ischemic damage measured by MRI was significantly negatively correlated with neuro-logical evaluations (stroke scores). “■”: M1 occlusion group; “▲”: M2 occlusion group; “—” : the linear regression line; “::::::::”: the 95% confidence band of the best-fit line.
Mentions: Both the MRI images and histological examination confirmed that the M1 occlusion group had a larger infarction volume than did the M2 occlusion group, and was associated with significantly lower neurological evaluation scores. Statistical analysis of infarction volume and neurological evaluation score showed a significant difference between the two groups (Figure 3A, B). The neurological evaluation scores after embolization were significantly negatively correlated with infarct volume (r = −0.8649, P = 0.0056) (Figure 4).

Bottom Line: Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients.However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies.The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

ABSTRACT
Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients. However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies. In this study, eight healthy adult rhesus monkeys were randomized into two groups with four monkeys in each group: middle cerebral artery occlusion at origin segment (M1) and middle cerebral artery occlusion at M2 segment. The blood flow in the middle cerebral artery was blocked completely for 2 hours using the endovascular microcoil placement technique (1 mm × 10 cm) (undetachable), to establish a model of cerebral ischemia. The microcoil was withdrawn and the middle cerebral artery blood flow was restored. A reversible middle cerebral artery occlusion model was identified by hematoxylin-eosin staining, digital subtraction angiography, magnetic resonance angiography, magnetic resonance imaging, and neurological evaluation. The results showed that the middle cerebral artery occlusion model was successfully established in eight adult healthy rhesus monkeys, and ischemic lesions were apparent in the brain tissue of rhesus monkeys at 24 hours after occlusion. The rhesus monkeys had symptoms of neurological deficits. Compared with the M1 occlusion group, the M2 occlusion group had lower infarction volume and higher neurological scores. These experimental findings indicate that reversible middle cerebral artery occlusion can be produced with the endovascular microcoil technique in rhesus monkeys. The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research.

No MeSH data available.


Related in: MedlinePlus