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Transplantation of human amniotic epithelial cells repairs brachial plexus injury: pathological and biomechanical analyses.

Yang Q, Luo M, Li P, Jin H - Neural Regen Res (2014)

Bottom Line: Immediately after the stretching, a suspension of human amniotic epithelial cells was injected into the injured brachial plexus.The treatment clearly improved the pathological morphology of the injured brachial plexus nerve, as seen by hematoxylin eosin staining, and the functions of the rabbit forepaw were restored.These data indicate that the injection of human amniotic epithelial cells contributed to the repair of brachial plexus injury, and that this technique may transform into current clinical treatment strategies.

View Article: PubMed Central - PubMed

Affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.

ABSTRACT
A brachial plexus injury model was established in rabbits by stretching the C6 nerve root. Immediately after the stretching, a suspension of human amniotic epithelial cells was injected into the injured brachial plexus. The results of tensile mechanical testing of the brachial plexus showed that the tensile elastic limit strain, elastic limit stress, maximum stress, and maximum strain of the injured brachial plexuses were significantly increased at 24 weeks after the injection. The treatment clearly improved the pathological morphology of the injured brachial plexus nerve, as seen by hematoxylin eosin staining, and the functions of the rabbit forepaw were restored. These data indicate that the injection of human amniotic epithelial cells contributed to the repair of brachial plexus injury, and that this technique may transform into current clinical treatment strategies.

No MeSH data available.


Related in: MedlinePlus

The stress-strain curves of the rabbit brachial plexus specimens in each group.hAECs: Human amniotic epithelial cells.
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Figure 1: The stress-strain curves of the rabbit brachial plexus specimens in each group.hAECs: Human amniotic epithelial cells.

Mentions: The stress-strain curves of the rabbit brachial plexus specimens from each group were plotted after the tensile testing data were fit to a general equation (Figure 1). In the control group, the stress-strain curves of the brachial plexus specimens were exponential from 0 to 7.62% strain, approximately linear from 7.63% to 15.80% strain, and experienced plastic deformation from 15.81% to 22.69% strain, losing load-bearing capacity and indicating tissue destruction. In the injury group, the stress-strain curves of the brachial plexus specimens were exponential from 0 to 4.21% strain, approximately linear from 4.22% to 10.13% strain, and experienced plastic deformation from 10.14% to 14.64% strain, losing load-bearing capacity and indicating tissue destruction. In the hAECs intervention group, the stress-strain curves were exponential from 0 to 7.53% strain, approximately linear from 7.54% to 14.54% strain, and experienced plastic deformation from 14.55% to 21.34% strain, losing load-bearing capacity and indicating tissue destruction.


Transplantation of human amniotic epithelial cells repairs brachial plexus injury: pathological and biomechanical analyses.

Yang Q, Luo M, Li P, Jin H - Neural Regen Res (2014)

The stress-strain curves of the rabbit brachial plexus specimens in each group.hAECs: Human amniotic epithelial cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316449&req=5

Figure 1: The stress-strain curves of the rabbit brachial plexus specimens in each group.hAECs: Human amniotic epithelial cells.
Mentions: The stress-strain curves of the rabbit brachial plexus specimens from each group were plotted after the tensile testing data were fit to a general equation (Figure 1). In the control group, the stress-strain curves of the brachial plexus specimens were exponential from 0 to 7.62% strain, approximately linear from 7.63% to 15.80% strain, and experienced plastic deformation from 15.81% to 22.69% strain, losing load-bearing capacity and indicating tissue destruction. In the injury group, the stress-strain curves of the brachial plexus specimens were exponential from 0 to 4.21% strain, approximately linear from 4.22% to 10.13% strain, and experienced plastic deformation from 10.14% to 14.64% strain, losing load-bearing capacity and indicating tissue destruction. In the hAECs intervention group, the stress-strain curves were exponential from 0 to 7.53% strain, approximately linear from 7.54% to 14.54% strain, and experienced plastic deformation from 14.55% to 21.34% strain, losing load-bearing capacity and indicating tissue destruction.

Bottom Line: Immediately after the stretching, a suspension of human amniotic epithelial cells was injected into the injured brachial plexus.The treatment clearly improved the pathological morphology of the injured brachial plexus nerve, as seen by hematoxylin eosin staining, and the functions of the rabbit forepaw were restored.These data indicate that the injection of human amniotic epithelial cells contributed to the repair of brachial plexus injury, and that this technique may transform into current clinical treatment strategies.

View Article: PubMed Central - PubMed

Affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.

ABSTRACT
A brachial plexus injury model was established in rabbits by stretching the C6 nerve root. Immediately after the stretching, a suspension of human amniotic epithelial cells was injected into the injured brachial plexus. The results of tensile mechanical testing of the brachial plexus showed that the tensile elastic limit strain, elastic limit stress, maximum stress, and maximum strain of the injured brachial plexuses were significantly increased at 24 weeks after the injection. The treatment clearly improved the pathological morphology of the injured brachial plexus nerve, as seen by hematoxylin eosin staining, and the functions of the rabbit forepaw were restored. These data indicate that the injection of human amniotic epithelial cells contributed to the repair of brachial plexus injury, and that this technique may transform into current clinical treatment strategies.

No MeSH data available.


Related in: MedlinePlus