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The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo.

Cheng Q, Jiang C, Wang C, Yu S, Zhang Q, Gu X, Ding F - Neural Regen Res (2014)

Bottom Line: We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity.Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2.All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.

ABSTRACT
We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achyranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

No MeSH data available.


Related in: MedlinePlus

Effects of ABPP fractions on cell viability of DRG cells.(A) Reverse phase-high-performance liquid chromatogram of ABPP, in which the column elutions were monitored at 220 nm. (B) Cell viability of cultured DRG cells after exposure to 12 ABPP fractions (II–XIII: ABPPa, b, c, d, e, f, g, h, i, j, k, and l) at 250 ng/mL or ABPP at 250 ng/mL (XIV) or 1 μg/mL (XV) for 24 hours. Data are presented as the mean ± SEM of three separate experiments (each in triplicate). *P < 0.05, vs. control (I, no treatment); #P < 0.05, vs. ABPPk (one-way analysis of variance plus Scheffé post hoc test). ABPP: Achyranthes bidentata polypeptides; DRG: dorsal root ganglion.
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Figure 1: Effects of ABPP fractions on cell viability of DRG cells.(A) Reverse phase-high-performance liquid chromatogram of ABPP, in which the column elutions were monitored at 220 nm. (B) Cell viability of cultured DRG cells after exposure to 12 ABPP fractions (II–XIII: ABPPa, b, c, d, e, f, g, h, i, j, k, and l) at 250 ng/mL or ABPP at 250 ng/mL (XIV) or 1 μg/mL (XV) for 24 hours. Data are presented as the mean ± SEM of three separate experiments (each in triplicate). *P < 0.05, vs. control (I, no treatment); #P < 0.05, vs. ABPPk (one-way analysis of variance plus Scheffé post hoc test). ABPP: Achyranthes bidentata polypeptides; DRG: dorsal root ganglion.

Mentions: A representative RP-HPLC chromatogram of ABPP was recorded at 220 nm, which indicated that a total of 12 fractions were isolated from ABPP. These components were eluted at different retention times and code-named ABPPa, b, c, d, e, f, g, h, i, j, k, and l (Figure 1A).


The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo.

Cheng Q, Jiang C, Wang C, Yu S, Zhang Q, Gu X, Ding F - Neural Regen Res (2014)

Effects of ABPP fractions on cell viability of DRG cells.(A) Reverse phase-high-performance liquid chromatogram of ABPP, in which the column elutions were monitored at 220 nm. (B) Cell viability of cultured DRG cells after exposure to 12 ABPP fractions (II–XIII: ABPPa, b, c, d, e, f, g, h, i, j, k, and l) at 250 ng/mL or ABPP at 250 ng/mL (XIV) or 1 μg/mL (XV) for 24 hours. Data are presented as the mean ± SEM of three separate experiments (each in triplicate). *P < 0.05, vs. control (I, no treatment); #P < 0.05, vs. ABPPk (one-way analysis of variance plus Scheffé post hoc test). ABPP: Achyranthes bidentata polypeptides; DRG: dorsal root ganglion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4316447&req=5

Figure 1: Effects of ABPP fractions on cell viability of DRG cells.(A) Reverse phase-high-performance liquid chromatogram of ABPP, in which the column elutions were monitored at 220 nm. (B) Cell viability of cultured DRG cells after exposure to 12 ABPP fractions (II–XIII: ABPPa, b, c, d, e, f, g, h, i, j, k, and l) at 250 ng/mL or ABPP at 250 ng/mL (XIV) or 1 μg/mL (XV) for 24 hours. Data are presented as the mean ± SEM of three separate experiments (each in triplicate). *P < 0.05, vs. control (I, no treatment); #P < 0.05, vs. ABPPk (one-way analysis of variance plus Scheffé post hoc test). ABPP: Achyranthes bidentata polypeptides; DRG: dorsal root ganglion.
Mentions: A representative RP-HPLC chromatogram of ABPP was recorded at 220 nm, which indicated that a total of 12 fractions were isolated from ABPP. These components were eluted at different retention times and code-named ABPPa, b, c, d, e, f, g, h, i, j, k, and l (Figure 1A).

Bottom Line: We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity.Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2.All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.

ABSTRACT
We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achyranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

No MeSH data available.


Related in: MedlinePlus