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A novel artificial nerve graft for repairing long-distance sciatic nerve defects: a self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit.

Wang X, Pan M, Wen J, Tang Y, Hamilton AD, Li Y, Qian C, Liu Z, Wu W, Guo J - Neural Regen Res (2014)

Bottom Line: In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat.Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit.Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.

ABSTRACT
In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.

No MeSH data available.


Related in: MedlinePlus

Histomorphometry of the neuromuscular junction in the gastrocnemius muscle of rats with sciatic nerve transection following nerve conduit implantation.α-BTX-stained longitudinal gastrocnemius muscle sections showing neuromuscular junctions in the naive group (A), PN group (B), SPC group (C), EPC group (D) and NG group (E). a–e are magnified images of the boxed areas in A–E. In the injured groups (B–E), the junctions were reduced in size to varying degrees, and were all significantly smaller than in the naive group (A). (F) Quantification showing the size of the neuromuscular junctions in each group. The data are expressed as the mean ± SD. One-way analysis of variance followed by Bonferroni post hoc comparison was used to analyze intergroup differences. *P < 0.05, N.D.P > 0.05. PN: Syngeneic peripheral nerve; SPC: self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit; EPC: empty poly(lactic-co-glycolic acid) conduit; NG: non-graft control.
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Figure 7: Histomorphometry of the neuromuscular junction in the gastrocnemius muscle of rats with sciatic nerve transection following nerve conduit implantation.α-BTX-stained longitudinal gastrocnemius muscle sections showing neuromuscular junctions in the naive group (A), PN group (B), SPC group (C), EPC group (D) and NG group (E). a–e are magnified images of the boxed areas in A–E. In the injured groups (B–E), the junctions were reduced in size to varying degrees, and were all significantly smaller than in the naive group (A). (F) Quantification showing the size of the neuromuscular junctions in each group. The data are expressed as the mean ± SD. One-way analysis of variance followed by Bonferroni post hoc comparison was used to analyze intergroup differences. *P < 0.05, N.D.P > 0.05. PN: Syngeneic peripheral nerve; SPC: self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit; EPC: empty poly(lactic-co-glycolic acid) conduit; NG: non-graft control.

Mentions: Using α-BTX-stained longitudinal gastrocnemius muscle sections, the neuromuscular junction was clearly visible. In the nerve injury groups, the junctions were reduced in size to varying degrees and were significantly smaller than in the naive group (P < 0.05). The NG group had the smallest junction, followed by the EPC and SPC groups. The neuromuscular junction was largest in the PN group. There were significant differences among the groups, with the exception of SPC vs. PN (Figure 7).


A novel artificial nerve graft for repairing long-distance sciatic nerve defects: a self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit.

Wang X, Pan M, Wen J, Tang Y, Hamilton AD, Li Y, Qian C, Liu Z, Wu W, Guo J - Neural Regen Res (2014)

Histomorphometry of the neuromuscular junction in the gastrocnemius muscle of rats with sciatic nerve transection following nerve conduit implantation.α-BTX-stained longitudinal gastrocnemius muscle sections showing neuromuscular junctions in the naive group (A), PN group (B), SPC group (C), EPC group (D) and NG group (E). a–e are magnified images of the boxed areas in A–E. In the injured groups (B–E), the junctions were reduced in size to varying degrees, and were all significantly smaller than in the naive group (A). (F) Quantification showing the size of the neuromuscular junctions in each group. The data are expressed as the mean ± SD. One-way analysis of variance followed by Bonferroni post hoc comparison was used to analyze intergroup differences. *P < 0.05, N.D.P > 0.05. PN: Syngeneic peripheral nerve; SPC: self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit; EPC: empty poly(lactic-co-glycolic acid) conduit; NG: non-graft control.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 7: Histomorphometry of the neuromuscular junction in the gastrocnemius muscle of rats with sciatic nerve transection following nerve conduit implantation.α-BTX-stained longitudinal gastrocnemius muscle sections showing neuromuscular junctions in the naive group (A), PN group (B), SPC group (C), EPC group (D) and NG group (E). a–e are magnified images of the boxed areas in A–E. In the injured groups (B–E), the junctions were reduced in size to varying degrees, and were all significantly smaller than in the naive group (A). (F) Quantification showing the size of the neuromuscular junctions in each group. The data are expressed as the mean ± SD. One-way analysis of variance followed by Bonferroni post hoc comparison was used to analyze intergroup differences. *P < 0.05, N.D.P > 0.05. PN: Syngeneic peripheral nerve; SPC: self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit; EPC: empty poly(lactic-co-glycolic acid) conduit; NG: non-graft control.
Mentions: Using α-BTX-stained longitudinal gastrocnemius muscle sections, the neuromuscular junction was clearly visible. In the nerve injury groups, the junctions were reduced in size to varying degrees and were significantly smaller than in the naive group (P < 0.05). The NG group had the smallest junction, followed by the EPC and SPC groups. The neuromuscular junction was largest in the PN group. There were significant differences among the groups, with the exception of SPC vs. PN (Figure 7).

Bottom Line: In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat.Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit.Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.

ABSTRACT
In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.

No MeSH data available.


Related in: MedlinePlus