Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin.
Bottom Line: We show that in addition to facing the basal lamina, dystroglycan is found near the nodal matrix around axons, binds matrix components, and participates in initial events of nodogenesis.These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier.Further, our data indicate that dystroglycan binds free matrix that is not organized in a basal lamina.
Affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.Show MeSH
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Mentions: By confocal microscopy, DG colocalizes with ezrin/radixin/moesin (ERM) proteins at SC microvilli (Occhi et al., 2005). To address whether DG is inserted solely in the SC membrane facing the basal lamina, or also in the nodal gap facing the axon, we performed IEM on sciatic nerves. Both α- and β-DG are found in SC microvilli not only adjacent to the basal lamina but also in the nodal gap abutting onto the axon (Fig. 4, A and B). Only occasional gold grains were found in DG-deficient mice (Fig. 4).
Affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.