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Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin.

Colombelli C, Palmisano M, Eshed-Eisenbach Y, Zambroni D, Pavoni E, Ferri C, Saccucci S, Nicole S, Soininen R, McKee KK, Yurchenco PD, Peles E, Wrabetz L, Feltri ML - J. Cell Biol. (2015)

Bottom Line: We show that in addition to facing the basal lamina, dystroglycan is found near the nodal matrix around axons, binds matrix components, and participates in initial events of nodogenesis.These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier.Further, our data indicate that dystroglycan binds free matrix that is not organized in a basal lamina.

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Affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.

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α- and β-DG localize in the nodal gap. IEM on sciatic nerve of wild-type (A and B) or dgko (C and D) adult mouse using antibodies against α-DG (A and C) and β-DG (B and D) shows gold particles decorating SC microvilli both near the basal lamina (arrows in A′ and B′, magnified at right), near the axon, and in the nodal gap (arrowheads in A′ and B′, magnified at right) only in wild-type nerves. Occasional gold grains are randomly distributed in the knockout nerves (C′ and D′, arrows). (E and F) Number of gold grains in each half/node. *, P < 0.02; ***, P < 0.0002; ****, P < 0.0001 by Student’s t test. Error bars represent SEM. Bars: (A–D) 1 µm; (A′–D′) 0.5 µm.
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fig4: α- and β-DG localize in the nodal gap. IEM on sciatic nerve of wild-type (A and B) or dgko (C and D) adult mouse using antibodies against α-DG (A and C) and β-DG (B and D) shows gold particles decorating SC microvilli both near the basal lamina (arrows in A′ and B′, magnified at right), near the axon, and in the nodal gap (arrowheads in A′ and B′, magnified at right) only in wild-type nerves. Occasional gold grains are randomly distributed in the knockout nerves (C′ and D′, arrows). (E and F) Number of gold grains in each half/node. *, P < 0.02; ***, P < 0.0002; ****, P < 0.0001 by Student’s t test. Error bars represent SEM. Bars: (A–D) 1 µm; (A′–D′) 0.5 µm.

Mentions: By confocal microscopy, DG colocalizes with ezrin/radixin/moesin (ERM) proteins at SC microvilli (Occhi et al., 2005). To address whether DG is inserted solely in the SC membrane facing the basal lamina, or also in the nodal gap facing the axon, we performed IEM on sciatic nerves. Both α- and β-DG are found in SC microvilli not only adjacent to the basal lamina but also in the nodal gap abutting onto the axon (Fig. 4, A and B). Only occasional gold grains were found in DG-deficient mice (Fig. 4).


Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin.

Colombelli C, Palmisano M, Eshed-Eisenbach Y, Zambroni D, Pavoni E, Ferri C, Saccucci S, Nicole S, Soininen R, McKee KK, Yurchenco PD, Peles E, Wrabetz L, Feltri ML - J. Cell Biol. (2015)

α- and β-DG localize in the nodal gap. IEM on sciatic nerve of wild-type (A and B) or dgko (C and D) adult mouse using antibodies against α-DG (A and C) and β-DG (B and D) shows gold particles decorating SC microvilli both near the basal lamina (arrows in A′ and B′, magnified at right), near the axon, and in the nodal gap (arrowheads in A′ and B′, magnified at right) only in wild-type nerves. Occasional gold grains are randomly distributed in the knockout nerves (C′ and D′, arrows). (E and F) Number of gold grains in each half/node. *, P < 0.02; ***, P < 0.0002; ****, P < 0.0001 by Student’s t test. Error bars represent SEM. Bars: (A–D) 1 µm; (A′–D′) 0.5 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

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fig4: α- and β-DG localize in the nodal gap. IEM on sciatic nerve of wild-type (A and B) or dgko (C and D) adult mouse using antibodies against α-DG (A and C) and β-DG (B and D) shows gold particles decorating SC microvilli both near the basal lamina (arrows in A′ and B′, magnified at right), near the axon, and in the nodal gap (arrowheads in A′ and B′, magnified at right) only in wild-type nerves. Occasional gold grains are randomly distributed in the knockout nerves (C′ and D′, arrows). (E and F) Number of gold grains in each half/node. *, P < 0.02; ***, P < 0.0002; ****, P < 0.0001 by Student’s t test. Error bars represent SEM. Bars: (A–D) 1 µm; (A′–D′) 0.5 µm.
Mentions: By confocal microscopy, DG colocalizes with ezrin/radixin/moesin (ERM) proteins at SC microvilli (Occhi et al., 2005). To address whether DG is inserted solely in the SC membrane facing the basal lamina, or also in the nodal gap facing the axon, we performed IEM on sciatic nerves. Both α- and β-DG are found in SC microvilli not only adjacent to the basal lamina but also in the nodal gap abutting onto the axon (Fig. 4, A and B). Only occasional gold grains were found in DG-deficient mice (Fig. 4).

Bottom Line: We show that in addition to facing the basal lamina, dystroglycan is found near the nodal matrix around axons, binds matrix components, and participates in initial events of nodogenesis.These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier.Further, our data indicate that dystroglycan binds free matrix that is not organized in a basal lamina.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.

Show MeSH
Related in: MedlinePlus