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Beta-catenin expression in psoriasis.

El-Wahed Gaber MA, El-Halim Kandil MA, El-Farargy SM, Galbet DA - Indian Dermatol Online J (2015 Jan-Feb)

Bottom Line: In this study membranous β-catenin expression was significantly demonstrated in the control group then the non-lesional areas in comparison to the lesional areas (P < 0.001).Nuclear β-catenin staining expression was significantly more demonstrated in lesional and non-lesional areas in comparison to the control cases (P < 0.001).Nuclear β-catenin expression was not found in the control group but was demonstrated in lesional and moderately in non-lesional reflecting its role in kerationcyte proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Andrology and Venoreology, Faculty of Medicine, Menoufia University, Menufia, Egypt.

ABSTRACT

Background: Psoriasis is a common inflammatory skin disease characterized by abnormal keratinocyte proliferation and differentiation. Beta-catenin participates in intercellular adhesion. Catenins are proteins found in complexes with cadherin cell adhesion molecules of cells. The role of catenin in regulating keratinocyte stem cell differentiation and hair follicle morphogenesis has been extensively reported.

Aims and objectives: is to study β-catenin expression in lesional and non-lesional psoriatic skin to throw light upon its possible role in the pathogenesis of psoriasis.

Materials and methods: Biopsies were taken from 20 patients with psoriasis vulgaris and from 10 normal controls. The distribution of Beta catenin was investigated using polycolonal rabbits B-catenin antibody-1 by immunohistochemical method.

Results: In this study membranous β-catenin expression was significantly demonstrated in the control group then the non-lesional areas in comparison to the lesional areas (P < 0.001). Nuclear β-catenin staining expression was significantly more demonstrated in lesional and non-lesional areas in comparison to the control cases (P < 0.001).

Conclusions: The down regulation of membranous β-catenin expression in lesional psoriatic skin might reflect a useful phenotypic marker of hyperprolifration of keratinocytes in psoriasis. Moreover, the mild down regulation of membranous β-catenin expression in non lesional psoriatic skin may provide clues about incipient structural abnormalities in the pathogenesis of psoriasis, providing an early diagnostic indicator for evolution to a generalized form of the disease. Nuclear β-catenin expression was not found in the control group but was demonstrated in lesional and moderately in non-lesional reflecting its role in kerationcyte proliferation.

No MeSH data available.


Related in: MedlinePlus

(a) Nuclear suprabasal beta-catenin (β-catenin) staining expression in all layers of epidermis except the parakeratotic and cornified layers of a psoriasis lesion (Immunoperioxedase stain, ×400). (b) Strong nuclear with membranous β-catenin expression and negative expression in the parakeratotic layer of a psoriasis lesion (Immunoperioxedase stain, ×400)
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Figure 3: (a) Nuclear suprabasal beta-catenin (β-catenin) staining expression in all layers of epidermis except the parakeratotic and cornified layers of a psoriasis lesion (Immunoperioxedase stain, ×400). (b) Strong nuclear with membranous β-catenin expression and negative expression in the parakeratotic layer of a psoriasis lesion (Immunoperioxedase stain, ×400)

Mentions: In the present study, we made a comparative study of the structural organization of the epidermis and in particular the intercellular junctions between lesional and nonlesional skin of psoriasis patients. The presence and distribution of β-catenin was analyzed by immunohistochemistry of normal skin, and lesional and nonlesional psoriatic skin. In the normal epidermis β-catenin was detected solely on the cell membrane of the basal cells and lower spinous cells [Table 1, Figure 2a]. This protein showed a continuous well defined distribution at the borders of these cells. In nonlesional psoriatic skin, there were a few suprabasal cells showing strong nuclear β-catenin expression [Table 2, Figure 2b]. In lesional psoriatic skin, there was a positive nuclear suprabasal β-catenin expression and negative β-catenin expression in parakeratotic cells [Table 2, Figure 3a]. There was a strong nuclear with membranous β-catenin expression in psoriatic skin [Figure 3b]. At the junction between nonlesional and lesional skin there was membranous β-catenin expression in nonlesional skin and both membranous and nuclear β-catenin expression in lesional psoriatic skin [Figure 4a]. In nonlesional psoriatic skin, there was a membranous β-catenin expression along with a positive dermal inflammatory component [Figure 4b]. There was positive nuclear and cytoplasmic β-catenin expression in normal hair follicles [Figure 4c].


Beta-catenin expression in psoriasis.

El-Wahed Gaber MA, El-Halim Kandil MA, El-Farargy SM, Galbet DA - Indian Dermatol Online J (2015 Jan-Feb)

(a) Nuclear suprabasal beta-catenin (β-catenin) staining expression in all layers of epidermis except the parakeratotic and cornified layers of a psoriasis lesion (Immunoperioxedase stain, ×400). (b) Strong nuclear with membranous β-catenin expression and negative expression in the parakeratotic layer of a psoriasis lesion (Immunoperioxedase stain, ×400)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4314880&req=5

Figure 3: (a) Nuclear suprabasal beta-catenin (β-catenin) staining expression in all layers of epidermis except the parakeratotic and cornified layers of a psoriasis lesion (Immunoperioxedase stain, ×400). (b) Strong nuclear with membranous β-catenin expression and negative expression in the parakeratotic layer of a psoriasis lesion (Immunoperioxedase stain, ×400)
Mentions: In the present study, we made a comparative study of the structural organization of the epidermis and in particular the intercellular junctions between lesional and nonlesional skin of psoriasis patients. The presence and distribution of β-catenin was analyzed by immunohistochemistry of normal skin, and lesional and nonlesional psoriatic skin. In the normal epidermis β-catenin was detected solely on the cell membrane of the basal cells and lower spinous cells [Table 1, Figure 2a]. This protein showed a continuous well defined distribution at the borders of these cells. In nonlesional psoriatic skin, there were a few suprabasal cells showing strong nuclear β-catenin expression [Table 2, Figure 2b]. In lesional psoriatic skin, there was a positive nuclear suprabasal β-catenin expression and negative β-catenin expression in parakeratotic cells [Table 2, Figure 3a]. There was a strong nuclear with membranous β-catenin expression in psoriatic skin [Figure 3b]. At the junction between nonlesional and lesional skin there was membranous β-catenin expression in nonlesional skin and both membranous and nuclear β-catenin expression in lesional psoriatic skin [Figure 4a]. In nonlesional psoriatic skin, there was a membranous β-catenin expression along with a positive dermal inflammatory component [Figure 4b]. There was positive nuclear and cytoplasmic β-catenin expression in normal hair follicles [Figure 4c].

Bottom Line: In this study membranous β-catenin expression was significantly demonstrated in the control group then the non-lesional areas in comparison to the lesional areas (P < 0.001).Nuclear β-catenin staining expression was significantly more demonstrated in lesional and non-lesional areas in comparison to the control cases (P < 0.001).Nuclear β-catenin expression was not found in the control group but was demonstrated in lesional and moderately in non-lesional reflecting its role in kerationcyte proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Andrology and Venoreology, Faculty of Medicine, Menoufia University, Menufia, Egypt.

ABSTRACT

Background: Psoriasis is a common inflammatory skin disease characterized by abnormal keratinocyte proliferation and differentiation. Beta-catenin participates in intercellular adhesion. Catenins are proteins found in complexes with cadherin cell adhesion molecules of cells. The role of catenin in regulating keratinocyte stem cell differentiation and hair follicle morphogenesis has been extensively reported.

Aims and objectives: is to study β-catenin expression in lesional and non-lesional psoriatic skin to throw light upon its possible role in the pathogenesis of psoriasis.

Materials and methods: Biopsies were taken from 20 patients with psoriasis vulgaris and from 10 normal controls. The distribution of Beta catenin was investigated using polycolonal rabbits B-catenin antibody-1 by immunohistochemical method.

Results: In this study membranous β-catenin expression was significantly demonstrated in the control group then the non-lesional areas in comparison to the lesional areas (P < 0.001). Nuclear β-catenin staining expression was significantly more demonstrated in lesional and non-lesional areas in comparison to the control cases (P < 0.001).

Conclusions: The down regulation of membranous β-catenin expression in lesional psoriatic skin might reflect a useful phenotypic marker of hyperprolifration of keratinocytes in psoriasis. Moreover, the mild down regulation of membranous β-catenin expression in non lesional psoriatic skin may provide clues about incipient structural abnormalities in the pathogenesis of psoriasis, providing an early diagnostic indicator for evolution to a generalized form of the disease. Nuclear β-catenin expression was not found in the control group but was demonstrated in lesional and moderately in non-lesional reflecting its role in kerationcyte proliferation.

No MeSH data available.


Related in: MedlinePlus