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Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus

Cell spatial arrangement. For better understanding of the g(r), cell arrangements are presented here. A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. A fewer neurons (white circles) and wider gaps were arranged around the central neuron (black circle) in the stressed rats. Similar results were also obtained with regards to the glial cells. Bivariate assessments or the cross-correlation of the neurons and glial cells in the control and stress+distilled water groups are presented in C and D, respectively. More dispersion and repulsion of the glial cells (black circles) can be seen around the central neuron (white circle) in the stressed animals.
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Figure 3: Cell spatial arrangement. For better understanding of the g(r), cell arrangements are presented here. A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. A fewer neurons (white circles) and wider gaps were arranged around the central neuron (black circle) in the stressed rats. Similar results were also obtained with regards to the glial cells. Bivariate assessments or the cross-correlation of the neurons and glial cells in the control and stress+distilled water groups are presented in C and D, respectively. More dispersion and repulsion of the glial cells (black circles) can be seen around the central neuron (white circle) in the stressed animals.

Mentions: Estimates of g(r) for the neurons are plotted against the dipole distances, r, in Figure 2A and B. The values at the beginning of the curve (from r=0 to 5.2 µm) showed a difference between the control (A) and stress+distilled water groups. It then reduced and at a value of about 7 µm, indicated by letter "R", crossed the reference line. After dipping to the minimum value, the plot rose above the reference value again. In the control group, the curve crossed the line at R=7 µm and rose at 23 µm. In the stressed group, the corresponding values were R=7 and 52 µm. As Figure 2 depicts, at these distances, the data points of the stressed rats went down below the reference line [g(r)=1] indicating a significant negative correlation between the neurons. This means that they were neither arranged randomly nor clustered; rather, they were repulsed. However, this did not occur in the controls and repulsion could be detectedonly in a smaller distance. After the gap, the data points in both groups showed a random arrangement at larger distances (p<0.05). Therefore, the spatial arrangement of the neurons was changed by stress and dissociation of the neurons could be seen at some places. This indicates that the neurons are not normally arranged after stress. For better understanding of the plots, cell arrangements are presented in Figure 3. Figure 3A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. According to the results, fewer neurons and wider gaps were detected in the stressed rats. The evaluation of the g(r) of the neuron of the control vs. stress+distilled water shows significant differences. No differences were seen between stress+distilled water vs. stress+olive oil. The group of curcumin or sertraline alone did not represent any significant differences in comparison with the control group. The g(r) of the neuron showed significant differences in the stress+sertraline or stress+curcumin groups in comparison with the stress+distilled water or stress+olive oil, respectively.


Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Cell spatial arrangement. For better understanding of the g(r), cell arrangements are presented here. A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. A fewer neurons (white circles) and wider gaps were arranged around the central neuron (black circle) in the stressed rats. Similar results were also obtained with regards to the glial cells. Bivariate assessments or the cross-correlation of the neurons and glial cells in the control and stress+distilled water groups are presented in C and D, respectively. More dispersion and repulsion of the glial cells (black circles) can be seen around the central neuron (white circle) in the stressed animals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4310924&req=5

Figure 3: Cell spatial arrangement. For better understanding of the g(r), cell arrangements are presented here. A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. A fewer neurons (white circles) and wider gaps were arranged around the central neuron (black circle) in the stressed rats. Similar results were also obtained with regards to the glial cells. Bivariate assessments or the cross-correlation of the neurons and glial cells in the control and stress+distilled water groups are presented in C and D, respectively. More dispersion and repulsion of the glial cells (black circles) can be seen around the central neuron (white circle) in the stressed animals.
Mentions: Estimates of g(r) for the neurons are plotted against the dipole distances, r, in Figure 2A and B. The values at the beginning of the curve (from r=0 to 5.2 µm) showed a difference between the control (A) and stress+distilled water groups. It then reduced and at a value of about 7 µm, indicated by letter "R", crossed the reference line. After dipping to the minimum value, the plot rose above the reference value again. In the control group, the curve crossed the line at R=7 µm and rose at 23 µm. In the stressed group, the corresponding values were R=7 and 52 µm. As Figure 2 depicts, at these distances, the data points of the stressed rats went down below the reference line [g(r)=1] indicating a significant negative correlation between the neurons. This means that they were neither arranged randomly nor clustered; rather, they were repulsed. However, this did not occur in the controls and repulsion could be detectedonly in a smaller distance. After the gap, the data points in both groups showed a random arrangement at larger distances (p<0.05). Therefore, the spatial arrangement of the neurons was changed by stress and dissociation of the neurons could be seen at some places. This indicates that the neurons are not normally arranged after stress. For better understanding of the plots, cell arrangements are presented in Figure 3. Figure 3A and B show the arrangement of the neurons in the control and stress+distilled water groups, respectively. According to the results, fewer neurons and wider gaps were detected in the stressed rats. The evaluation of the g(r) of the neuron of the control vs. stress+distilled water shows significant differences. No differences were seen between stress+distilled water vs. stress+olive oil. The group of curcumin or sertraline alone did not represent any significant differences in comparison with the control group. The g(r) of the neuron showed significant differences in the stress+sertraline or stress+curcumin groups in comparison with the stress+distilled water or stress+olive oil, respectively.

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus