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Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus

Pair correlation, and cross correlation functions g(r) of the neurons and glia. In each plot the mean over five cases is plotted with a point-wise confidence interval based on Students's t-distribution. The relationship between pair correlation function and dipole distance for neurons in the control (A) and stress+distilled water (B) rats. The horizontal reference ( hypothesis) line corresponded to the values expected for a random spatial arrangement [g(r)=1]. The pair correlation function approached closely to the reference line because no significant correlation was found between the values of points lying far away in the cortical tissue. The relationship between pair correlation function and dipole distance for glial cells in the control (C) and stress+distilled water groups (D). The cross-correlation, g(r), between the neurons and glial cells plotted versus distance, r, in micrometers in the control (E) and stress+distilled water (F) groups. The dots are the mean g(r) of the six animals in the control and stress+distilled water groups. The horizontal reference line at g(r)=1.0 is the reference line expected for a 'random' structure. "▾" and "▴" indicate gmax and gmin, respectively in both groups.
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Figure 2: Pair correlation, and cross correlation functions g(r) of the neurons and glia. In each plot the mean over five cases is plotted with a point-wise confidence interval based on Students's t-distribution. The relationship between pair correlation function and dipole distance for neurons in the control (A) and stress+distilled water (B) rats. The horizontal reference ( hypothesis) line corresponded to the values expected for a random spatial arrangement [g(r)=1]. The pair correlation function approached closely to the reference line because no significant correlation was found between the values of points lying far away in the cortical tissue. The relationship between pair correlation function and dipole distance for glial cells in the control (C) and stress+distilled water groups (D). The cross-correlation, g(r), between the neurons and glial cells plotted versus distance, r, in micrometers in the control (E) and stress+distilled water (F) groups. The dots are the mean g(r) of the six animals in the control and stress+distilled water groups. The horizontal reference line at g(r)=1.0 is the reference line expected for a 'random' structure. "▾" and "▴" indicate gmax and gmin, respectively in both groups.

Mentions: In addition to correlation and cross-correlation, to estimate the degree of order of the neurons and glial cells in mPFC, the statistic "M" was estimated in both groups. It is related to the global degree of order in the spatial point pattern.25,26 This estimation includes identification of the 1stgmax and the next following minimum gmin with the corresponding r-values rmax and rmin (Figure 2) for each reduced g-function, where rmin>rmax. Using these data, the statistic will be estimated by the following formula: M=(gmax-gmin)/(rmin-rmax)


Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Pair correlation, and cross correlation functions g(r) of the neurons and glia. In each plot the mean over five cases is plotted with a point-wise confidence interval based on Students's t-distribution. The relationship between pair correlation function and dipole distance for neurons in the control (A) and stress+distilled water (B) rats. The horizontal reference ( hypothesis) line corresponded to the values expected for a random spatial arrangement [g(r)=1]. The pair correlation function approached closely to the reference line because no significant correlation was found between the values of points lying far away in the cortical tissue. The relationship between pair correlation function and dipole distance for glial cells in the control (C) and stress+distilled water groups (D). The cross-correlation, g(r), between the neurons and glial cells plotted versus distance, r, in micrometers in the control (E) and stress+distilled water (F) groups. The dots are the mean g(r) of the six animals in the control and stress+distilled water groups. The horizontal reference line at g(r)=1.0 is the reference line expected for a 'random' structure. "▾" and "▴" indicate gmax and gmin, respectively in both groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4310924&req=5

Figure 2: Pair correlation, and cross correlation functions g(r) of the neurons and glia. In each plot the mean over five cases is plotted with a point-wise confidence interval based on Students's t-distribution. The relationship between pair correlation function and dipole distance for neurons in the control (A) and stress+distilled water (B) rats. The horizontal reference ( hypothesis) line corresponded to the values expected for a random spatial arrangement [g(r)=1]. The pair correlation function approached closely to the reference line because no significant correlation was found between the values of points lying far away in the cortical tissue. The relationship between pair correlation function and dipole distance for glial cells in the control (C) and stress+distilled water groups (D). The cross-correlation, g(r), between the neurons and glial cells plotted versus distance, r, in micrometers in the control (E) and stress+distilled water (F) groups. The dots are the mean g(r) of the six animals in the control and stress+distilled water groups. The horizontal reference line at g(r)=1.0 is the reference line expected for a 'random' structure. "▾" and "▴" indicate gmax and gmin, respectively in both groups.
Mentions: In addition to correlation and cross-correlation, to estimate the degree of order of the neurons and glial cells in mPFC, the statistic "M" was estimated in both groups. It is related to the global degree of order in the spatial point pattern.25,26 This estimation includes identification of the 1stgmax and the next following minimum gmin with the corresponding r-values rmax and rmin (Figure 2) for each reduced g-function, where rmin>rmax. Using these data, the statistic will be estimated by the following formula: M=(gmax-gmin)/(rmin-rmax)

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus