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Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus

Coding of the cells for stereological estimation. A transparent lattice bearing one row of points was constructed to serve as a set of dipole probes. The point interval (r) corresponded to a distance of 2.6 µm. Each test point was coded as 1,2, and 3 if the point was laid on the neurons, glia, and neuropil, respectively.
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Figure 1: Coding of the cells for stereological estimation. A transparent lattice bearing one row of points was constructed to serve as a set of dipole probes. The point interval (r) corresponded to a distance of 2.6 µm. Each test point was coded as 1,2, and 3 if the point was laid on the neurons, glia, and neuropil, respectively.

Mentions: The stereological objective is to estimate the local 3D arrangements of different tissue compartments within mPFC. A transparent lattice bearing a row of points was constructed to serve as a set of linear dipole probes.21,22,23,24,25,26 Each row consisted of 50 points and 49 equidistant intervals. The point interval corresponded to a distance of 2.6 µm on the scale of the tissue. This distance was selected because it offers a sensible starting distance with reference to the size of the glial cells. Consequently, both end points of dipoles (DP) of class size r=1 (equivalent to 2.6 µm) have chances of being included within the same cell section. That was also true for the neurons. The lattice was superimposed on the live image on a monitor connected to a microscope (Nikon, E-200, Japan). It was set to be random in position and orientation in each field. For each mPFC, 11 trials (a total of 550 test points) were conducted. For every trial, the nature of the tissue component underlying each test point was noted and all this information was recorded on a 50×11 matrix printed as a blank table with 50 columns and 11 rows. Within the cells of this matrix, each point was coded as 1 (neuron), 2 (glial cell), and 3 (neuropil) (Figure 1). There after the data sets were analyzed using the software prepared in Histomorphometry and Stereology Research Centre, Shiraz, Iran. To estimate "Vv", "C(r)", and "g(r)", the distance between the points (DP) ranged from r=0 (equivalent to 0 µm) to r=49 and the total distance was 49×2.6=127.4 µm. The covariance of a component (X) was estimated by the following equation: C(r) X=ΣDP (X, r)/ΣDP (ref, r) where DP(X, r) and DP (ref, r) are the dipole length which hit the favored structure (neuron or glia) and the nervous tissue, respectively.


Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it.

Noorafshan A, Abdollahifar MA, Karbalay-Doust S - Psychiatry Investig (2015)

Coding of the cells for stereological estimation. A transparent lattice bearing one row of points was constructed to serve as a set of dipole probes. The point interval (r) corresponded to a distance of 2.6 µm. Each test point was coded as 1,2, and 3 if the point was laid on the neurons, glia, and neuropil, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4310924&req=5

Figure 1: Coding of the cells for stereological estimation. A transparent lattice bearing one row of points was constructed to serve as a set of dipole probes. The point interval (r) corresponded to a distance of 2.6 µm. Each test point was coded as 1,2, and 3 if the point was laid on the neurons, glia, and neuropil, respectively.
Mentions: The stereological objective is to estimate the local 3D arrangements of different tissue compartments within mPFC. A transparent lattice bearing a row of points was constructed to serve as a set of linear dipole probes.21,22,23,24,25,26 Each row consisted of 50 points and 49 equidistant intervals. The point interval corresponded to a distance of 2.6 µm on the scale of the tissue. This distance was selected because it offers a sensible starting distance with reference to the size of the glial cells. Consequently, both end points of dipoles (DP) of class size r=1 (equivalent to 2.6 µm) have chances of being included within the same cell section. That was also true for the neurons. The lattice was superimposed on the live image on a monitor connected to a microscope (Nikon, E-200, Japan). It was set to be random in position and orientation in each field. For each mPFC, 11 trials (a total of 550 test points) were conducted. For every trial, the nature of the tissue component underlying each test point was noted and all this information was recorded on a 50×11 matrix printed as a blank table with 50 columns and 11 rows. Within the cells of this matrix, each point was coded as 1 (neuron), 2 (glial cell), and 3 (neuropil) (Figure 1). There after the data sets were analyzed using the software prepared in Histomorphometry and Stereology Research Centre, Shiraz, Iran. To estimate "Vv", "C(r)", and "g(r)", the distance between the points (DP) ranged from r=0 (equivalent to 0 µm) to r=49 and the total distance was 49×2.6=127.4 µm. The covariance of a component (X) was estimated by the following equation: C(r) X=ΣDP (X, r)/ΣDP (ref, r) where DP(X, r) and DP (ref, r) are the dipole length which hit the favored structure (neuron or glia) and the nervous tissue, respectively.

Bottom Line: The corresponding values decreased by 50% in the stressed group.Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group.In addition, the volume of the neurons and glial cells remained unchanged after stress.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. ; Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: The present study explored the three-dimensional spatial arrangements of the neurons and glial cells within the medial prefrontal cortex (mPFC) of rats.

Methods: It evaluated the arrangement for differences after stress with or without treatment with curcumin and sertraline using second-order stereology. Orientator method was applied to obtain isotropic uniform random sections of mPFC. The pair correlation g(r) and cross-correlation functions were estimated by counting dipole probes superimposed on histological sections of mPFC.

Results: The mean total volume of neurons and glial cells was 0.80 (0.05) and 0.40 (0.07), respectively in the control group. The corresponding values decreased by 50% in the stressed group. The curve of g(r) for the neurons and glial cells showed a wider gap between the stressed rats' mPFC. Theses indicate a negative correlation (repulsion) between the neurons and glial cells in the stressed rats. Evaluation of the cross-correlation function of the neurons and glial cells also showed a negative correlation in the stressed group. The estimated values of the global degree of order in the spatial point pattern for neurons and glial cells were 0.62 and 0.20 in control and stressed animals, respectively. Curcumin and sertraline protected the spatial arrangements of the cells after stress induction in rats. In addition, the volume of the neurons and glial cells remained unchanged after stress.

Conclusion: Dissociation of the neurons and glial cells can is seen at some places in the stressed rats' cortex. However, the spatial arrangement of the cells was remained unchanged in curcumin+stress and sertraline+stress rats.

No MeSH data available.


Related in: MedlinePlus