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Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis.

Davidson R, Laporte D, Wu JQ - Mol. Biol. Cell (2014)

Bottom Line: Using an Rgf3 conditional mutant and mislocalization experiments, we found that Art1 and Rgf3 are interdependent for localization to the division site.As expected, active Rho1 levels at the division site are reduced in art1∆ and rgf3 mutant cells.Taken together, these data reveal that the arrestin family protein Art1 regulates the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels during fission yeast cytokinesis.

View Article: PubMed Central - PubMed

Affiliation: Graduate Program of Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, OH 43210 Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.

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A model for the role of Art1 in regulating Rgf3 and Rho1 functions. Rgf3 localization, Rho1 activation, and septal formation are compared in art1+ (left) versus art1∆ (right) cells. See the Discussion for details.
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Figure 7: A model for the role of Art1 in regulating Rgf3 and Rho1 functions. Rgf3 localization, Rho1 activation, and septal formation are compared in art1+ (left) versus art1∆ (right) cells. See the Discussion for details.

Mentions: Our data suggest a model in which Art1 recruits Rgf3 to the division site, where it catalyzes the exchange of GDP for GTP on Rho1 GTPase (Figure 7). GEF activity of Rgf3 leads to increased levels of active Rho1, which in turn activates the β-glucan synthases Bgs1 and Bgs4 for septal and cell wall synthesis (Figure 7, left). In art1∆ cells, low levels of Rgf3 at the division site lead to inefficient Rho1 activation, which in turn leads to poor activation of β-glucan synthases, resulting in an impaired septum and cell lysis (Figure 7, right). The fact that Art1 does not make a significant contribution toward Bgs1 localization suggests that Rho1 plays no prominent role in Bgs1 localization. Bgs1 localization may be dependent on the exocyst complex, Cdc42, and the F-BAR protein Cdc15 (Bendezu and Martin, 2011; Arasada and Pollard, 2014).


Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis.

Davidson R, Laporte D, Wu JQ - Mol. Biol. Cell (2014)

A model for the role of Art1 in regulating Rgf3 and Rho1 functions. Rgf3 localization, Rho1 activation, and septal formation are compared in art1+ (left) versus art1∆ (right) cells. See the Discussion for details.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4310737&req=5

Figure 7: A model for the role of Art1 in regulating Rgf3 and Rho1 functions. Rgf3 localization, Rho1 activation, and septal formation are compared in art1+ (left) versus art1∆ (right) cells. See the Discussion for details.
Mentions: Our data suggest a model in which Art1 recruits Rgf3 to the division site, where it catalyzes the exchange of GDP for GTP on Rho1 GTPase (Figure 7). GEF activity of Rgf3 leads to increased levels of active Rho1, which in turn activates the β-glucan synthases Bgs1 and Bgs4 for septal and cell wall synthesis (Figure 7, left). In art1∆ cells, low levels of Rgf3 at the division site lead to inefficient Rho1 activation, which in turn leads to poor activation of β-glucan synthases, resulting in an impaired septum and cell lysis (Figure 7, right). The fact that Art1 does not make a significant contribution toward Bgs1 localization suggests that Rho1 plays no prominent role in Bgs1 localization. Bgs1 localization may be dependent on the exocyst complex, Cdc42, and the F-BAR protein Cdc15 (Bendezu and Martin, 2011; Arasada and Pollard, 2014).

Bottom Line: Using an Rgf3 conditional mutant and mislocalization experiments, we found that Art1 and Rgf3 are interdependent for localization to the division site.As expected, active Rho1 levels at the division site are reduced in art1∆ and rgf3 mutant cells.Taken together, these data reveal that the arrestin family protein Art1 regulates the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels during fission yeast cytokinesis.

View Article: PubMed Central - PubMed

Affiliation: Graduate Program of Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, OH 43210 Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.

Show MeSH
Related in: MedlinePlus