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Glycosaminoglycans in the human cornea: age-related changes.

Pacella E, Pacella F, De Paolis G, Parisella FR, Turchetti P, Anello G, Cavallotti C - Ophthalmol Eye Dis (2015)

Bottom Line: All the quantitative results were statistically analyzed.The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects.Their quantity is significantly altered in the elderly in comparison with younger subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Sense Organs, Faculty of Medicine and Dentistry, Sapienza University of Rome, Rome, Italy.

ABSTRACT

Aim: To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides.

Methods: Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed.

Results: The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects.

Discussion: GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea.

No MeSH data available.


Related in: MedlinePlus

Comparison of the GAG content of young and old human corneas: the proportion of hyaluronic acid (HA) was higher in the young corneas, while the proportion of sulfated GAGs (ChS, DS, KS, HS) was lower in young corneas as compared to old corneas.
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f2-oed-7-2015-001: Comparison of the GAG content of young and old human corneas: the proportion of hyaluronic acid (HA) was higher in the young corneas, while the proportion of sulfated GAGs (ChS, DS, KS, HS) was lower in young corneas as compared to old corneas.

Mentions: By contrast, the sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, strongly increased in the older subjects, by about 30–40% with the exception of the heparan sulfate, which increased by about 80%. All the data present in Table 2 were statistically significant (P < 0.05) (Figs. 2 and 3).


Glycosaminoglycans in the human cornea: age-related changes.

Pacella E, Pacella F, De Paolis G, Parisella FR, Turchetti P, Anello G, Cavallotti C - Ophthalmol Eye Dis (2015)

Comparison of the GAG content of young and old human corneas: the proportion of hyaluronic acid (HA) was higher in the young corneas, while the proportion of sulfated GAGs (ChS, DS, KS, HS) was lower in young corneas as compared to old corneas.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4310673&req=5

f2-oed-7-2015-001: Comparison of the GAG content of young and old human corneas: the proportion of hyaluronic acid (HA) was higher in the young corneas, while the proportion of sulfated GAGs (ChS, DS, KS, HS) was lower in young corneas as compared to old corneas.
Mentions: By contrast, the sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, strongly increased in the older subjects, by about 30–40% with the exception of the heparan sulfate, which increased by about 80%. All the data present in Table 2 were statistically significant (P < 0.05) (Figs. 2 and 3).

Bottom Line: All the quantitative results were statistically analyzed.The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects.Their quantity is significantly altered in the elderly in comparison with younger subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Sense Organs, Faculty of Medicine and Dentistry, Sapienza University of Rome, Rome, Italy.

ABSTRACT

Aim: To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides.

Methods: Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed.

Results: The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects.

Discussion: GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea.

No MeSH data available.


Related in: MedlinePlus