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Glycosaminoglycans in the human cornea: age-related changes.

Pacella E, Pacella F, De Paolis G, Parisella FR, Turchetti P, Anello G, Cavallotti C - Ophthalmol Eye Dis (2015)

Bottom Line: All the quantitative results were statistically analyzed.The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects.Their quantity is significantly altered in the elderly in comparison with younger subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Sense Organs, Faculty of Medicine and Dentistry, Sapienza University of Rome, Rome, Italy.

ABSTRACT

Aim: To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides.

Methods: Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed.

Results: The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects.

Discussion: GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea.

No MeSH data available.


Related in: MedlinePlus

Comparison of total GAG content of young and old human corneas: Total GAG content was significantly higher in old corneas as compared to young corneas (<0,05).
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f1-oed-7-2015-001: Comparison of total GAG content of young and old human corneas: Total GAG content was significantly higher in old corneas as compared to young corneas (<0,05).

Mentions: Table 2 contains the biochemical values related to the quantity of GAGs in the corneal homogenates of the two age groups of subjects. As can be seen, the total GAGs count was 97.4 ± 6.2 mg of hexuronic acid per gram of protein. In the younger subjects, the GAG count was 127.3 ± 8.5 mg of hexuronic acid per gram of protein, while in the older subjects, there was a significant increase of about 30% compared to younger corneas (Fig. 1).


Glycosaminoglycans in the human cornea: age-related changes.

Pacella E, Pacella F, De Paolis G, Parisella FR, Turchetti P, Anello G, Cavallotti C - Ophthalmol Eye Dis (2015)

Comparison of total GAG content of young and old human corneas: Total GAG content was significantly higher in old corneas as compared to young corneas (<0,05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4310673&req=5

f1-oed-7-2015-001: Comparison of total GAG content of young and old human corneas: Total GAG content was significantly higher in old corneas as compared to young corneas (<0,05).
Mentions: Table 2 contains the biochemical values related to the quantity of GAGs in the corneal homogenates of the two age groups of subjects. As can be seen, the total GAGs count was 97.4 ± 6.2 mg of hexuronic acid per gram of protein. In the younger subjects, the GAG count was 127.3 ± 8.5 mg of hexuronic acid per gram of protein, while in the older subjects, there was a significant increase of about 30% compared to younger corneas (Fig. 1).

Bottom Line: All the quantitative results were statistically analyzed.The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects.Their quantity is significantly altered in the elderly in comparison with younger subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Sense Organs, Faculty of Medicine and Dentistry, Sapienza University of Rome, Rome, Italy.

ABSTRACT

Aim: To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides.

Methods: Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed.

Results: The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects.

Discussion: GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea.

No MeSH data available.


Related in: MedlinePlus