Limits...
The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss.

Ricketts CD, Bates WR, Reid SD - PLoS ONE (2015)

Bottom Line: Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls.These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish.Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Irving K. Barber School of Arts and Sciences, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, British Columbia, Canada, V1V 1V7.

ABSTRACT
To determine if molybdenum (Mo) is a chemical stressor, fingerling and juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne sodium molybdate (0, 2, 20, or 1,000 mg l-1 of Mo) and components of the physiological (plasma cortisol, blood glucose, and hematocrit) and cellular (heat shock protein [hsp] 72, hsp73, and hsp90 in the liver, gills, heart, and erythrocytes and metallothionein [MT] in the liver and gills) stress responses were measured prior to initiation of exposure and at 8, 24, and 96 h. During the acute exposure, plasma cortisol, blood glucose, and hematocrit levels remained unchanged in all treatments. Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls. Both fingerling and juvenile fish responded with similar lack of apparent sensitivity to Mo exposure. These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish. Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

Show MeSH

Related in: MedlinePlus

Liver (A), gill (B), heart (C), and erythrocyte (D) hsp90 levels (relative to positive control) of juvenile rainbow trout exposed for 96 h to waterborne Mo at concentrations of 0, 2, or 20 mg l-1.Data plotted as means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups. Representative Western blots for total hsp70 in the each tissues are shown above their respective bar graphs with the location of location of the MagicMark XP Western Protein Standard indicated on the left side of each blot (in kDa).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309612&req=5

pone.0115334.g006: Liver (A), gill (B), heart (C), and erythrocyte (D) hsp90 levels (relative to positive control) of juvenile rainbow trout exposed for 96 h to waterborne Mo at concentrations of 0, 2, or 20 mg l-1.Data plotted as means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups. Representative Western blots for total hsp70 in the each tissues are shown above their respective bar graphs with the location of location of the MagicMark XP Western Protein Standard indicated on the left side of each blot (in kDa).

Mentions: Figs. 5 and 6 show that the expression of total hsp70 (72 and 73) and hsp90 in the liver (A), gills (B), heart (C), and erythrocytes (D) of juvenile rainbow trout remained at control levels after the 96 h exposure to 2 or 20 mg l-1. Fingerling fish sampled prior to addition of Mo did not differ in their liver and gill total hsp70 (Fig. 7A, B) or liver hsp90 (Fig. 8) levels, and these levels did not change when sampled at 8, 24, and 96 h during exposure.


The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss.

Ricketts CD, Bates WR, Reid SD - PLoS ONE (2015)

Liver (A), gill (B), heart (C), and erythrocyte (D) hsp90 levels (relative to positive control) of juvenile rainbow trout exposed for 96 h to waterborne Mo at concentrations of 0, 2, or 20 mg l-1.Data plotted as means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups. Representative Western blots for total hsp70 in the each tissues are shown above their respective bar graphs with the location of location of the MagicMark XP Western Protein Standard indicated on the left side of each blot (in kDa).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309612&req=5

pone.0115334.g006: Liver (A), gill (B), heart (C), and erythrocyte (D) hsp90 levels (relative to positive control) of juvenile rainbow trout exposed for 96 h to waterborne Mo at concentrations of 0, 2, or 20 mg l-1.Data plotted as means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups. Representative Western blots for total hsp70 in the each tissues are shown above their respective bar graphs with the location of location of the MagicMark XP Western Protein Standard indicated on the left side of each blot (in kDa).
Mentions: Figs. 5 and 6 show that the expression of total hsp70 (72 and 73) and hsp90 in the liver (A), gills (B), heart (C), and erythrocytes (D) of juvenile rainbow trout remained at control levels after the 96 h exposure to 2 or 20 mg l-1. Fingerling fish sampled prior to addition of Mo did not differ in their liver and gill total hsp70 (Fig. 7A, B) or liver hsp90 (Fig. 8) levels, and these levels did not change when sampled at 8, 24, and 96 h during exposure.

Bottom Line: Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls.These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish.Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Irving K. Barber School of Arts and Sciences, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, British Columbia, Canada, V1V 1V7.

ABSTRACT
To determine if molybdenum (Mo) is a chemical stressor, fingerling and juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne sodium molybdate (0, 2, 20, or 1,000 mg l-1 of Mo) and components of the physiological (plasma cortisol, blood glucose, and hematocrit) and cellular (heat shock protein [hsp] 72, hsp73, and hsp90 in the liver, gills, heart, and erythrocytes and metallothionein [MT] in the liver and gills) stress responses were measured prior to initiation of exposure and at 8, 24, and 96 h. During the acute exposure, plasma cortisol, blood glucose, and hematocrit levels remained unchanged in all treatments. Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls. Both fingerling and juvenile fish responded with similar lack of apparent sensitivity to Mo exposure. These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish. Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

Show MeSH
Related in: MedlinePlus