Limits...
The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss.

Ricketts CD, Bates WR, Reid SD - PLoS ONE (2015)

Bottom Line: Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls.These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish.Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Irving K. Barber School of Arts and Sciences, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, British Columbia, Canada, V1V 1V7.

ABSTRACT
To determine if molybdenum (Mo) is a chemical stressor, fingerling and juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne sodium molybdate (0, 2, 20, or 1,000 mg l-1 of Mo) and components of the physiological (plasma cortisol, blood glucose, and hematocrit) and cellular (heat shock protein [hsp] 72, hsp73, and hsp90 in the liver, gills, heart, and erythrocytes and metallothionein [MT] in the liver and gills) stress responses were measured prior to initiation of exposure and at 8, 24, and 96 h. During the acute exposure, plasma cortisol, blood glucose, and hematocrit levels remained unchanged in all treatments. Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls. Both fingerling and juvenile fish responded with similar lack of apparent sensitivity to Mo exposure. These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish. Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

Show MeSH

Related in: MedlinePlus

Erythrocyte hsp72 levels in from juvenile rainbow trout exposed to molybdenum (0, 2, or 20 mg l-1) for 96 h.(A) Representative Western blot of hsp72 expression in juvenile rainbow trout erythrocytes. (B) Erythrocyte hsp72 levels (relative to positive control). Data plotted means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309612&req=5

pone.0115334.g004: Erythrocyte hsp72 levels in from juvenile rainbow trout exposed to molybdenum (0, 2, or 20 mg l-1) for 96 h.(A) Representative Western blot of hsp72 expression in juvenile rainbow trout erythrocytes. (B) Erythrocyte hsp72 levels (relative to positive control). Data plotted means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups.

Mentions: Immunoblotting was carried out with a panel of hsp antibodies on cell free extracts of liver, gill, heart, and erythrocyte samples from fish exposed to Mo. There was no detectable induction of hsp72 under control conditions or after 96 h of exposure to 2 or 20 mg l-1 in the liver, gills, or heart of juvenile fish. The erythrocytes, however, in both the control group and the Mo exposure groups expressed basal levels of hsp72 at about 30–50% of the heat shocked positive control (Fig. 4). Even at 1,000 mg l-1 there was no detectable induction of hsp72 in the liver or gills of fingerling fish.


The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss.

Ricketts CD, Bates WR, Reid SD - PLoS ONE (2015)

Erythrocyte hsp72 levels in from juvenile rainbow trout exposed to molybdenum (0, 2, or 20 mg l-1) for 96 h.(A) Representative Western blot of hsp72 expression in juvenile rainbow trout erythrocytes. (B) Erythrocyte hsp72 levels (relative to positive control). Data plotted means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309612&req=5

pone.0115334.g004: Erythrocyte hsp72 levels in from juvenile rainbow trout exposed to molybdenum (0, 2, or 20 mg l-1) for 96 h.(A) Representative Western blot of hsp72 expression in juvenile rainbow trout erythrocytes. (B) Erythrocyte hsp72 levels (relative to positive control). Data plotted means ± 1 SEM (n = 6). There were no significant differences (p > 0.05) between groups.
Mentions: Immunoblotting was carried out with a panel of hsp antibodies on cell free extracts of liver, gill, heart, and erythrocyte samples from fish exposed to Mo. There was no detectable induction of hsp72 under control conditions or after 96 h of exposure to 2 or 20 mg l-1 in the liver, gills, or heart of juvenile fish. The erythrocytes, however, in both the control group and the Mo exposure groups expressed basal levels of hsp72 at about 30–50% of the heat shocked positive control (Fig. 4). Even at 1,000 mg l-1 there was no detectable induction of hsp72 in the liver or gills of fingerling fish.

Bottom Line: Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls.These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish.Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Irving K. Barber School of Arts and Sciences, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, British Columbia, Canada, V1V 1V7.

ABSTRACT
To determine if molybdenum (Mo) is a chemical stressor, fingerling and juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne sodium molybdate (0, 2, 20, or 1,000 mg l-1 of Mo) and components of the physiological (plasma cortisol, blood glucose, and hematocrit) and cellular (heat shock protein [hsp] 72, hsp73, and hsp90 in the liver, gills, heart, and erythrocytes and metallothionein [MT] in the liver and gills) stress responses were measured prior to initiation of exposure and at 8, 24, and 96 h. During the acute exposure, plasma cortisol, blood glucose, and hematocrit levels remained unchanged in all treatments. Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls. Both fingerling and juvenile fish responded with similar lack of apparent sensitivity to Mo exposure. These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l(-1) did not activate a physiological or cellular stress response in fish. Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

Show MeSH
Related in: MedlinePlus