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Structural associations of symptomatic knee osteoarthritis.

Stoppiello LA, Mapp PI, Wilson D, Hill R, Scammell BE, Walsh DA - (2014)

Bottom Line: In this study, we aimed to identify histopathologic features that are associated with symptomatic knee OA.To identify features of OA, we compared the patients with advanced OA with the age-matched non-OA controls (n = 26 per group).Synovitis, increased synovial NGF, alterations in chondrocyte morphology, and loss of cartilage integrity are features of knee OA that may be associated with symptoms.

View Article: PubMed Central - PubMed

Affiliation: Arthritis Research UK Pain Centre and University of Nottingham, Nottingham, UK.

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Cellular localization of nerve growth factor (NGF) immunoreactivity in human synovium. A, Hsp47 immunoreactivity localized to cells with fibroblast morphology in the lining and sublining regions of synovium from a patient with osteoarthritis (OA). Arrow indicates a fibroblast colocalized to NGF (same cell as indicated in B); arrowhead indicates a fibroblast not colocalized to NGF. B, NGF immunoreactivity colocalized with Hsp47 (arrow) and in a cell that is not immunolabeled for Hsp47 (arrowhead) in the section shown in A. C, CD68 immunoreactivity of macrophages in synovium from a patient with OA. Arrow indicates a macrophage colocalized to NGF (same cell as indicated in D); arrowhead indicates a macrophage not colocalized to NGF. D, NGF immunoreactivity colocalized with CD68 (arrow) and in a cell that is not immunolabeled for CD68 (arrowhead) in the section shown in C. Double immunohistochemistry was visualized by Texas Red (Hsp47 and CD68 [red]) and fluorescein (NGF [green]) staining. Dotted lines indicate the synovial surface. Bars = 50 μm.
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fig02: Cellular localization of nerve growth factor (NGF) immunoreactivity in human synovium. A, Hsp47 immunoreactivity localized to cells with fibroblast morphology in the lining and sublining regions of synovium from a patient with osteoarthritis (OA). Arrow indicates a fibroblast colocalized to NGF (same cell as indicated in B); arrowhead indicates a fibroblast not colocalized to NGF. B, NGF immunoreactivity colocalized with Hsp47 (arrow) and in a cell that is not immunolabeled for Hsp47 (arrowhead) in the section shown in A. C, CD68 immunoreactivity of macrophages in synovium from a patient with OA. Arrow indicates a macrophage colocalized to NGF (same cell as indicated in D); arrowhead indicates a macrophage not colocalized to NGF. D, NGF immunoreactivity colocalized with CD68 (arrow) and in a cell that is not immunolabeled for CD68 (arrowhead) in the section shown in C. Double immunohistochemistry was visualized by Texas Red (Hsp47 and CD68 [red]) and fluorescein (NGF [green]) staining. Dotted lines indicate the synovial surface. Bars = 50 μm.

Mentions: NGF immunoreactivity in the synovium was predominantly localized to Hsp47+ spindle-shaped and mononuclear cells, consistent with fibroblast morphology (Figures 2A and B). However, some NGF+Hsp47– and NGF–Hsp47+ cells were also observed. Some CD68+macrophages in the lining and sublining regions also displayed NGF immunoreactivity (Figures 2C and D). Similar distributions were found in advanced OA patients and non-OA controls.


Structural associations of symptomatic knee osteoarthritis.

Stoppiello LA, Mapp PI, Wilson D, Hill R, Scammell BE, Walsh DA - (2014)

Cellular localization of nerve growth factor (NGF) immunoreactivity in human synovium. A, Hsp47 immunoreactivity localized to cells with fibroblast morphology in the lining and sublining regions of synovium from a patient with osteoarthritis (OA). Arrow indicates a fibroblast colocalized to NGF (same cell as indicated in B); arrowhead indicates a fibroblast not colocalized to NGF. B, NGF immunoreactivity colocalized with Hsp47 (arrow) and in a cell that is not immunolabeled for Hsp47 (arrowhead) in the section shown in A. C, CD68 immunoreactivity of macrophages in synovium from a patient with OA. Arrow indicates a macrophage colocalized to NGF (same cell as indicated in D); arrowhead indicates a macrophage not colocalized to NGF. D, NGF immunoreactivity colocalized with CD68 (arrow) and in a cell that is not immunolabeled for CD68 (arrowhead) in the section shown in C. Double immunohistochemistry was visualized by Texas Red (Hsp47 and CD68 [red]) and fluorescein (NGF [green]) staining. Dotted lines indicate the synovial surface. Bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309484&req=5

fig02: Cellular localization of nerve growth factor (NGF) immunoreactivity in human synovium. A, Hsp47 immunoreactivity localized to cells with fibroblast morphology in the lining and sublining regions of synovium from a patient with osteoarthritis (OA). Arrow indicates a fibroblast colocalized to NGF (same cell as indicated in B); arrowhead indicates a fibroblast not colocalized to NGF. B, NGF immunoreactivity colocalized with Hsp47 (arrow) and in a cell that is not immunolabeled for Hsp47 (arrowhead) in the section shown in A. C, CD68 immunoreactivity of macrophages in synovium from a patient with OA. Arrow indicates a macrophage colocalized to NGF (same cell as indicated in D); arrowhead indicates a macrophage not colocalized to NGF. D, NGF immunoreactivity colocalized with CD68 (arrow) and in a cell that is not immunolabeled for CD68 (arrowhead) in the section shown in C. Double immunohistochemistry was visualized by Texas Red (Hsp47 and CD68 [red]) and fluorescein (NGF [green]) staining. Dotted lines indicate the synovial surface. Bars = 50 μm.
Mentions: NGF immunoreactivity in the synovium was predominantly localized to Hsp47+ spindle-shaped and mononuclear cells, consistent with fibroblast morphology (Figures 2A and B). However, some NGF+Hsp47– and NGF–Hsp47+ cells were also observed. Some CD68+macrophages in the lining and sublining regions also displayed NGF immunoreactivity (Figures 2C and D). Similar distributions were found in advanced OA patients and non-OA controls.

Bottom Line: In this study, we aimed to identify histopathologic features that are associated with symptomatic knee OA.To identify features of OA, we compared the patients with advanced OA with the age-matched non-OA controls (n = 26 per group).Synovitis, increased synovial NGF, alterations in chondrocyte morphology, and loss of cartilage integrity are features of knee OA that may be associated with symptoms.

View Article: PubMed Central - PubMed

Affiliation: Arthritis Research UK Pain Centre and University of Nottingham, Nottingham, UK.

Show MeSH
Related in: MedlinePlus