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Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.

Xie LJ, Yu LJ, Chen QF, Wang FZ, Huang L, Xia FN, Zhu TR, Wu JX, Yin J, Liao B, Yao N, Shu W, Xiao S - Plant J. (2014)

Bottom Line: ACBP3-overexpressors (ACBP3-OEs) showed hypersensitivity to DS, LS and ethanolic stresses, with reduced transcription of hypoxia-responsive genes as well as accumulation of hydrogen peroxide in the rosettes.Lipid profiling revealed that both the total amounts and very-long-chain species of phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure.Further, a knockout mutant of MYB30, a master regulator of very-long-chain fatty acid (VLCFA) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses, phenotypes that were ameliorated by ACBP3-RNAi.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

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Phenotypes of ACBP3-OEs and ACBP3-KOs to hypoxic stress.(a) Phenotypes of 4-week-old wild type (WT), ACBP3-OEs (OE-1 and OE-4), ACBP3-KOs (acbp3 and ACBP3-RNAi) plants before treatment (day 0) and after 2-day (for ACBP3-OEs) or 3-day (for ACBP3-KOs) DS treatment, followed by 3 days' recovery.(b, c) Survival rates (b) and dry weights (c) of WT, ACBP3-OEs and ACBP3-KOs after DS treatment followed by 3 days' recovery.(d) Phenotypes of 4-week-old WT, ACBP3-OEs, ACBP3-KOs before treatment (day 0) and after 5 days (for ACBP3-OEs) or 7 days (for ACBP3-KOs) of LS treatment, followed by 3 days' recovery.(e, f) Survival rates (e) and dry weights (f) of the WT, ACBP3-OEs and ACBP3-KOs after LS treatment followed by 3 days' recovery.Bars represent means ± SD (n = 3) of three independent experiments (for one experiment, >15 plants were scored for each genotype). Asterisks indicate significant differences from WT; **P < 0.01 by Student's t-test.
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fig02: Phenotypes of ACBP3-OEs and ACBP3-KOs to hypoxic stress.(a) Phenotypes of 4-week-old wild type (WT), ACBP3-OEs (OE-1 and OE-4), ACBP3-KOs (acbp3 and ACBP3-RNAi) plants before treatment (day 0) and after 2-day (for ACBP3-OEs) or 3-day (for ACBP3-KOs) DS treatment, followed by 3 days' recovery.(b, c) Survival rates (b) and dry weights (c) of WT, ACBP3-OEs and ACBP3-KOs after DS treatment followed by 3 days' recovery.(d) Phenotypes of 4-week-old WT, ACBP3-OEs, ACBP3-KOs before treatment (day 0) and after 5 days (for ACBP3-OEs) or 7 days (for ACBP3-KOs) of LS treatment, followed by 3 days' recovery.(e, f) Survival rates (e) and dry weights (f) of the WT, ACBP3-OEs and ACBP3-KOs after LS treatment followed by 3 days' recovery.Bars represent means ± SD (n = 3) of three independent experiments (for one experiment, >15 plants were scored for each genotype). Asterisks indicate significant differences from WT; **P < 0.01 by Student's t-test.

Mentions: To investigate the role of ACBP3 in hypoxia response, wild-type, ACBP3-OEs and ACBP3-KOs plants were used to determine their tolerance to hypoxic stress. The growth and development of 4-week-old ACBP3-OEs and ACBP3-KOs lines was indistinguishable from that of wild type (Figure 2a). When the plant lines were DS-treated for 2 days or LS-treated for 5 days plus a 3-day recovery period, two ACBP3-OEs (OE-1 and OE-4) showed increased sensitivity to submergence in comparison with wild type (Figure 2a,d). In contrast, when wild type and ACBP3-KOs (acbp3 and ACBP3-RNAi) were treated for 3 days under DS or for 7 days under LS, most ACBP3-KOs survived, whereas most wild-type plants died (Figure 2a,d), indicating that depletion of ACBP3 in ACBP3-KOs improved plant tolerance to submergence. The attenuated and enhanced hypoxic tolerance of ACBP3-OEs and ACBP3-KOs, respectively, was further confirmed by analyses of the survival rates (Figure 2b,e) and dry weights (Figure 2c,f) of DS- or LS-treated plants followed by a 3-day recovery. As shown in Figure 2, both survival rates and dry weights of ACBP3-OEs were significantly lower than wild type upon treatment with either 2-day DS or 5-day LS. In contrast, data of both ACBP3-KOs were significantly higher than wild type under the extended treatment with 3-day DS or 7-day LS (Figure 2).


Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.

Xie LJ, Yu LJ, Chen QF, Wang FZ, Huang L, Xia FN, Zhu TR, Wu JX, Yin J, Liao B, Yao N, Shu W, Xiao S - Plant J. (2014)

Phenotypes of ACBP3-OEs and ACBP3-KOs to hypoxic stress.(a) Phenotypes of 4-week-old wild type (WT), ACBP3-OEs (OE-1 and OE-4), ACBP3-KOs (acbp3 and ACBP3-RNAi) plants before treatment (day 0) and after 2-day (for ACBP3-OEs) or 3-day (for ACBP3-KOs) DS treatment, followed by 3 days' recovery.(b, c) Survival rates (b) and dry weights (c) of WT, ACBP3-OEs and ACBP3-KOs after DS treatment followed by 3 days' recovery.(d) Phenotypes of 4-week-old WT, ACBP3-OEs, ACBP3-KOs before treatment (day 0) and after 5 days (for ACBP3-OEs) or 7 days (for ACBP3-KOs) of LS treatment, followed by 3 days' recovery.(e, f) Survival rates (e) and dry weights (f) of the WT, ACBP3-OEs and ACBP3-KOs after LS treatment followed by 3 days' recovery.Bars represent means ± SD (n = 3) of three independent experiments (for one experiment, >15 plants were scored for each genotype). Asterisks indicate significant differences from WT; **P < 0.01 by Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig02: Phenotypes of ACBP3-OEs and ACBP3-KOs to hypoxic stress.(a) Phenotypes of 4-week-old wild type (WT), ACBP3-OEs (OE-1 and OE-4), ACBP3-KOs (acbp3 and ACBP3-RNAi) plants before treatment (day 0) and after 2-day (for ACBP3-OEs) or 3-day (for ACBP3-KOs) DS treatment, followed by 3 days' recovery.(b, c) Survival rates (b) and dry weights (c) of WT, ACBP3-OEs and ACBP3-KOs after DS treatment followed by 3 days' recovery.(d) Phenotypes of 4-week-old WT, ACBP3-OEs, ACBP3-KOs before treatment (day 0) and after 5 days (for ACBP3-OEs) or 7 days (for ACBP3-KOs) of LS treatment, followed by 3 days' recovery.(e, f) Survival rates (e) and dry weights (f) of the WT, ACBP3-OEs and ACBP3-KOs after LS treatment followed by 3 days' recovery.Bars represent means ± SD (n = 3) of three independent experiments (for one experiment, >15 plants were scored for each genotype). Asterisks indicate significant differences from WT; **P < 0.01 by Student's t-test.
Mentions: To investigate the role of ACBP3 in hypoxia response, wild-type, ACBP3-OEs and ACBP3-KOs plants were used to determine their tolerance to hypoxic stress. The growth and development of 4-week-old ACBP3-OEs and ACBP3-KOs lines was indistinguishable from that of wild type (Figure 2a). When the plant lines were DS-treated for 2 days or LS-treated for 5 days plus a 3-day recovery period, two ACBP3-OEs (OE-1 and OE-4) showed increased sensitivity to submergence in comparison with wild type (Figure 2a,d). In contrast, when wild type and ACBP3-KOs (acbp3 and ACBP3-RNAi) were treated for 3 days under DS or for 7 days under LS, most ACBP3-KOs survived, whereas most wild-type plants died (Figure 2a,d), indicating that depletion of ACBP3 in ACBP3-KOs improved plant tolerance to submergence. The attenuated and enhanced hypoxic tolerance of ACBP3-OEs and ACBP3-KOs, respectively, was further confirmed by analyses of the survival rates (Figure 2b,e) and dry weights (Figure 2c,f) of DS- or LS-treated plants followed by a 3-day recovery. As shown in Figure 2, both survival rates and dry weights of ACBP3-OEs were significantly lower than wild type upon treatment with either 2-day DS or 5-day LS. In contrast, data of both ACBP3-KOs were significantly higher than wild type under the extended treatment with 3-day DS or 7-day LS (Figure 2).

Bottom Line: ACBP3-overexpressors (ACBP3-OEs) showed hypersensitivity to DS, LS and ethanolic stresses, with reduced transcription of hypoxia-responsive genes as well as accumulation of hydrogen peroxide in the rosettes.Lipid profiling revealed that both the total amounts and very-long-chain species of phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure.Further, a knockout mutant of MYB30, a master regulator of very-long-chain fatty acid (VLCFA) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses, phenotypes that were ameliorated by ACBP3-RNAi.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

Show MeSH
Related in: MedlinePlus