Limits...
Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

Show MeSH

Related in: MedlinePlus

Scatter diagram of RBC aspect ratio versus MCV (fL) estimated from the MMT interpretation of the MWT spectra of RBCs samples from healthy donors, anemic patients, and laboratory RBC cultures infected with P. falciparum at different life cycle stages and the corresponding control noninfected RBCs.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4309305&req=5

fig6: Scatter diagram of RBC aspect ratio versus MCV (fL) estimated from the MMT interpretation of the MWT spectra of RBCs samples from healthy donors, anemic patients, and laboratory RBC cultures infected with P. falciparum at different life cycle stages and the corresponding control noninfected RBCs.

Mentions: Graphic illustration of how diseased RBCs can be readily distinguished from normal healthy RBCs using morphological parameters is presented in Figure 6. It shows scatter diagram of RBC aspect ratio versus MCV for all samples reviewed in this study. There is a clear tight pattern for the distribution of healthy cells on the plot whereas other cells produced considerably greater scatter. Since about a half of the samples from anemic patients had thin RBCs (microcytic or macrocytic), the data points corresponding to anemic patients fell largely below the tight distribution of the data from healthy patients. The data points corresponding to the parasite infected RBCs showed gradual increase in both parameters (volume and aspect ratio) with parasites' growth until mature trophozoite stage. The data points corresponding to the parasite infected RBCs at schizont stage are remarkably different, with low MCV but still high aspect ratio values.


Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Scatter diagram of RBC aspect ratio versus MCV (fL) estimated from the MMT interpretation of the MWT spectra of RBCs samples from healthy donors, anemic patients, and laboratory RBC cultures infected with P. falciparum at different life cycle stages and the corresponding control noninfected RBCs.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309305&req=5

fig6: Scatter diagram of RBC aspect ratio versus MCV (fL) estimated from the MMT interpretation of the MWT spectra of RBCs samples from healthy donors, anemic patients, and laboratory RBC cultures infected with P. falciparum at different life cycle stages and the corresponding control noninfected RBCs.
Mentions: Graphic illustration of how diseased RBCs can be readily distinguished from normal healthy RBCs using morphological parameters is presented in Figure 6. It shows scatter diagram of RBC aspect ratio versus MCV for all samples reviewed in this study. There is a clear tight pattern for the distribution of healthy cells on the plot whereas other cells produced considerably greater scatter. Since about a half of the samples from anemic patients had thin RBCs (microcytic or macrocytic), the data points corresponding to anemic patients fell largely below the tight distribution of the data from healthy patients. The data points corresponding to the parasite infected RBCs showed gradual increase in both parameters (volume and aspect ratio) with parasites' growth until mature trophozoite stage. The data points corresponding to the parasite infected RBCs at schizont stage are remarkably different, with low MCV but still high aspect ratio values.

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

Show MeSH
Related in: MedlinePlus