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Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

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Related in: MedlinePlus

Regressions between selected RBC indices ((a) MCV (fL), (b) MCHC (g/dL), (c) HCT (%), and (d) HGB (g/dL)) obtained through the MMT interpretation of the MWT spectra (y-axis) and those obtained with the reference SYSMEX SF-3000 analyzer (x-axis) for 43 samples from healthy donors and 51 samples from anemic patients.
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Related In: Results  -  Collection


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fig3: Regressions between selected RBC indices ((a) MCV (fL), (b) MCHC (g/dL), (c) HCT (%), and (d) HGB (g/dL)) obtained through the MMT interpretation of the MWT spectra (y-axis) and those obtained with the reference SYSMEX SF-3000 analyzer (x-axis) for 43 samples from healthy donors and 51 samples from anemic patients.

Mentions: The values for selected RBC indices (MCV, MCHC, HCT, and HGB) obtained from the interpretation of the MWT spectra and those from the reference SYSMEX SF-3000 analyzer are compared in Figure 3. The results of the statistical analysis for all RBC indices for the samples from healthy donors are given in Table 2 and for the samples from anemic patients are given in Table 3.


Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Regressions between selected RBC indices ((a) MCV (fL), (b) MCHC (g/dL), (c) HCT (%), and (d) HGB (g/dL)) obtained through the MMT interpretation of the MWT spectra (y-axis) and those obtained with the reference SYSMEX SF-3000 analyzer (x-axis) for 43 samples from healthy donors and 51 samples from anemic patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309305&req=5

fig3: Regressions between selected RBC indices ((a) MCV (fL), (b) MCHC (g/dL), (c) HCT (%), and (d) HGB (g/dL)) obtained through the MMT interpretation of the MWT spectra (y-axis) and those obtained with the reference SYSMEX SF-3000 analyzer (x-axis) for 43 samples from healthy donors and 51 samples from anemic patients.
Mentions: The values for selected RBC indices (MCV, MCHC, HCT, and HGB) obtained from the interpretation of the MWT spectra and those from the reference SYSMEX SF-3000 analyzer are compared in Figure 3. The results of the statistical analysis for all RBC indices for the samples from healthy donors are given in Table 2 and for the samples from anemic patients are given in Table 3.

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

Show MeSH
Related in: MedlinePlus