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Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

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Related in: MedlinePlus

Illustration of the interpretation of the measured MWT spectra τ (measured) of RBC fractions. The measured spectra τ (measured) are compared to the spectra reconstructed with the MMT based interpretation model τ (calculated) and σ (res) denotes the difference between the measured and calculated spectra. The reconstructed spectra of two orientation populations are also shown.
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fig2: Illustration of the interpretation of the measured MWT spectra τ (measured) of RBC fractions. The measured spectra τ (measured) are compared to the spectra reconstructed with the MMT based interpretation model τ (calculated) and σ (res) denotes the difference between the measured and calculated spectra. The reconstructed spectra of two orientation populations are also shown.

Mentions: The interpretation of the MWT spectra of RBC fractions with MMT model is illustrated with examples in Figure 2. Each figure contrasts a measured MWT spectrum and the corresponding spectrum calculated with the MMT model. The calculated spectrum was predicted as a sum of spectral contributions from two RBC orientation populations also shown in the figures. Excellent agreement between the calculated spectra and the corresponding measured MWT spectra can be noted. The RBC parameters (length and width) derived from the interpretation analysis of MWT spectra and calculated RBC indices are presented in the side panels.


Characterization of red blood cells with multiwavelength transmission spectroscopy.

Serebrennikova YM, Huffman DE, Garcia-Rubio LH - Biomed Res Int (2015)

Illustration of the interpretation of the measured MWT spectra τ (measured) of RBC fractions. The measured spectra τ (measured) are compared to the spectra reconstructed with the MMT based interpretation model τ (calculated) and σ (res) denotes the difference between the measured and calculated spectra. The reconstructed spectra of two orientation populations are also shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309305&req=5

fig2: Illustration of the interpretation of the measured MWT spectra τ (measured) of RBC fractions. The measured spectra τ (measured) are compared to the spectra reconstructed with the MMT based interpretation model τ (calculated) and σ (res) denotes the difference between the measured and calculated spectra. The reconstructed spectra of two orientation populations are also shown.
Mentions: The interpretation of the MWT spectra of RBC fractions with MMT model is illustrated with examples in Figure 2. Each figure contrasts a measured MWT spectrum and the corresponding spectrum calculated with the MMT model. The calculated spectrum was predicted as a sum of spectral contributions from two RBC orientation populations also shown in the figures. Excellent agreement between the calculated spectra and the corresponding measured MWT spectra can be noted. The RBC parameters (length and width) derived from the interpretation analysis of MWT spectra and calculated RBC indices are presented in the side panels.

Bottom Line: The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions.The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

View Article: PubMed Central - PubMed

Affiliation: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA.

ABSTRACT
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.

Show MeSH
Related in: MedlinePlus