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A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8.

Fengler S, Spirer I, Neef M, Ecke M, Nieselt K, Hampp R - Biomed Res Int (2015)

Bottom Line: The results show a major impact of nonmicrogravity related spaceflight conditions.In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity.These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.

View Article: PubMed Central - PubMed

Affiliation: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.

ABSTRACT
The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1 g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.

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Related in: MedlinePlus

Photograph of Arabidopsis thaliana semisolid callus cultures after a 5-day µg cultivation in orbit ((a), FS), 1 g in-flight cultivation ((b), FC) or on ground ((c), GS). The photographs were taken after fixation by RNAlater and recovery of the spacecraft.
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fig7: Photograph of Arabidopsis thaliana semisolid callus cultures after a 5-day µg cultivation in orbit ((a), FS), 1 g in-flight cultivation ((b), FC) or on ground ((c), GS). The photographs were taken after fixation by RNAlater and recovery of the spacecraft.

Mentions: The hardware was thoroughly tested in order to retain viability of the callus cultures for as long as possible. These tests were focused on the biocompatibility of the used materials, gas-exchange properties of membranes, and viability of the cell cultures under the cultivation conditions within the EC. We also recorded the oxygen content within the CC [37]. As this declined from 8 to about 2 mg/L after 5 days, automated sample fixation was set at day 5 after take-off. Mission parameters, such as temperature, were within nominal range during the mission. Radiation measurements recorded increased values. After landing and return of the biological material to the University of Tübingen (Germany), the samples were visually checked. The fixed calli showed good morphology and had well grown during the initial culture of 5 days in space. The calli from the 1 g controls (flight and ground experiment) were smaller compared to those exposed to microgravity (Figure 7).


A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8.

Fengler S, Spirer I, Neef M, Ecke M, Nieselt K, Hampp R - Biomed Res Int (2015)

Photograph of Arabidopsis thaliana semisolid callus cultures after a 5-day µg cultivation in orbit ((a), FS), 1 g in-flight cultivation ((b), FC) or on ground ((c), GS). The photographs were taken after fixation by RNAlater and recovery of the spacecraft.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309294&req=5

fig7: Photograph of Arabidopsis thaliana semisolid callus cultures after a 5-day µg cultivation in orbit ((a), FS), 1 g in-flight cultivation ((b), FC) or on ground ((c), GS). The photographs were taken after fixation by RNAlater and recovery of the spacecraft.
Mentions: The hardware was thoroughly tested in order to retain viability of the callus cultures for as long as possible. These tests were focused on the biocompatibility of the used materials, gas-exchange properties of membranes, and viability of the cell cultures under the cultivation conditions within the EC. We also recorded the oxygen content within the CC [37]. As this declined from 8 to about 2 mg/L after 5 days, automated sample fixation was set at day 5 after take-off. Mission parameters, such as temperature, were within nominal range during the mission. Radiation measurements recorded increased values. After landing and return of the biological material to the University of Tübingen (Germany), the samples were visually checked. The fixed calli showed good morphology and had well grown during the initial culture of 5 days in space. The calli from the 1 g controls (flight and ground experiment) were smaller compared to those exposed to microgravity (Figure 7).

Bottom Line: The results show a major impact of nonmicrogravity related spaceflight conditions.In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity.These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.

View Article: PubMed Central - PubMed

Affiliation: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.

ABSTRACT
The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1 g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.

Show MeSH
Related in: MedlinePlus