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Regulation of ICAM-1 in cells of the monocyte/macrophage system in microgravity.

Paulsen K, Tauber S, Dumrese C, Bradacs G, Simmet DM, Gölz N, Hauschild S, Raig C, Engeli S, Gutewort A, Hürlimann E, Biskup J, Unverdorben F, Rieder G, Hofmänner D, Mutschler L, Krammer S, Buttron I, Philpot C, Huge A, Lier H, Barz I, Engelmann F, Layer LE, Thiel CS, Ullrich O - Biomed Res Int (2015)

Bottom Line: In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission.In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments.Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.

ABSTRACT
Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte/macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

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Influence of altered gravity during parabolic flight and sounding rocket flight on ICAM-1 mRNA expression levels. (a) ICAM-1 mRNA expression levels are demonstrated for samples of the 19th DLR parabolic flight campaign after 1 g (light gray), 1.8 g (dark gray), μg (black), and hardware ground controls (H/W, striped) exposure and (b) for samples of the TEXUS-49 campaign after launch and acceleration (BL, dark gray), μg (black), and hardware ground controls (H/W, striped). ICAM-1 fluorescence intensities do not show any significant differences for all compared conditions in both experimental setups. The number of analyzed arrays: 19th DLR PFC: 1 g (n = 8), 1.8 g (n = 6), μg (n = 8) and H/W (n = 6); TEXUS-49: H/W (n = 6), μg (n = 7), BL (n = 5).
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fig7: Influence of altered gravity during parabolic flight and sounding rocket flight on ICAM-1 mRNA expression levels. (a) ICAM-1 mRNA expression levels are demonstrated for samples of the 19th DLR parabolic flight campaign after 1 g (light gray), 1.8 g (dark gray), μg (black), and hardware ground controls (H/W, striped) exposure and (b) for samples of the TEXUS-49 campaign after launch and acceleration (BL, dark gray), μg (black), and hardware ground controls (H/W, striped). ICAM-1 fluorescence intensities do not show any significant differences for all compared conditions in both experimental setups. The number of analyzed arrays: 19th DLR PFC: 1 g (n = 8), 1.8 g (n = 6), μg (n = 8) and H/W (n = 6); TEXUS-49: H/W (n = 6), μg (n = 7), BL (n = 5).

Mentions: RNA samples were analyzed for their quantity and quality and further processed for the microarray hybridization on 12 × 135 K Roche NimbleGen arrays. Data from 46 single microarrays (19th DLR PFC: 8x μg, 6x H/W, 8x 1 g, and 6x 1.8 g; TEXUS-49: 7x μg, 6x H/W, and 5x BL) were collected, normalized, and further analyzed. The data tables were screened for ICAM-1 values and mean fluorescence intensities including standard deviations were calculated for all samples of one condition. ICAM-1 shows stable expression for all gravity conditions during the 19th DLR PFC and the TEXUS-49 campaign, as well as for the H/W controls (Figure 7), indicating that microgravity and hypergravity conditions did not have an influence on mRNA ICAM-1 level in the range of 20 seconds until 6 minutes.


Regulation of ICAM-1 in cells of the monocyte/macrophage system in microgravity.

Paulsen K, Tauber S, Dumrese C, Bradacs G, Simmet DM, Gölz N, Hauschild S, Raig C, Engeli S, Gutewort A, Hürlimann E, Biskup J, Unverdorben F, Rieder G, Hofmänner D, Mutschler L, Krammer S, Buttron I, Philpot C, Huge A, Lier H, Barz I, Engelmann F, Layer LE, Thiel CS, Ullrich O - Biomed Res Int (2015)

Influence of altered gravity during parabolic flight and sounding rocket flight on ICAM-1 mRNA expression levels. (a) ICAM-1 mRNA expression levels are demonstrated for samples of the 19th DLR parabolic flight campaign after 1 g (light gray), 1.8 g (dark gray), μg (black), and hardware ground controls (H/W, striped) exposure and (b) for samples of the TEXUS-49 campaign after launch and acceleration (BL, dark gray), μg (black), and hardware ground controls (H/W, striped). ICAM-1 fluorescence intensities do not show any significant differences for all compared conditions in both experimental setups. The number of analyzed arrays: 19th DLR PFC: 1 g (n = 8), 1.8 g (n = 6), μg (n = 8) and H/W (n = 6); TEXUS-49: H/W (n = 6), μg (n = 7), BL (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
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fig7: Influence of altered gravity during parabolic flight and sounding rocket flight on ICAM-1 mRNA expression levels. (a) ICAM-1 mRNA expression levels are demonstrated for samples of the 19th DLR parabolic flight campaign after 1 g (light gray), 1.8 g (dark gray), μg (black), and hardware ground controls (H/W, striped) exposure and (b) for samples of the TEXUS-49 campaign after launch and acceleration (BL, dark gray), μg (black), and hardware ground controls (H/W, striped). ICAM-1 fluorescence intensities do not show any significant differences for all compared conditions in both experimental setups. The number of analyzed arrays: 19th DLR PFC: 1 g (n = 8), 1.8 g (n = 6), μg (n = 8) and H/W (n = 6); TEXUS-49: H/W (n = 6), μg (n = 7), BL (n = 5).
Mentions: RNA samples were analyzed for their quantity and quality and further processed for the microarray hybridization on 12 × 135 K Roche NimbleGen arrays. Data from 46 single microarrays (19th DLR PFC: 8x μg, 6x H/W, 8x 1 g, and 6x 1.8 g; TEXUS-49: 7x μg, 6x H/W, and 5x BL) were collected, normalized, and further analyzed. The data tables were screened for ICAM-1 values and mean fluorescence intensities including standard deviations were calculated for all samples of one condition. ICAM-1 shows stable expression for all gravity conditions during the 19th DLR PFC and the TEXUS-49 campaign, as well as for the H/W controls (Figure 7), indicating that microgravity and hypergravity conditions did not have an influence on mRNA ICAM-1 level in the range of 20 seconds until 6 minutes.

Bottom Line: In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission.In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments.Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.

ABSTRACT
Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte/macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

Show MeSH
Related in: MedlinePlus