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Space flight effects on antioxidant molecules in dry tardigrades: the TARDIKISS experiment.

Rizzo AM, Altiero T, Corsetto PA, Montorfano G, Guidetti R, Rebecchi L - Biomed Res Int (2015)

Bottom Line: In this paper we compared the molecular pathways of some antioxidant molecules, thiobarbituric acid reactive substances, and fatty acid composition between flight and control samples in two tardigrade species, namely, Paramacrobiotus richtersi and Ramazzottius oberhaeuseri.In both species, the activities of ROS scavenging enzymes, the total content of glutathione, and the fatty acids composition between flight and control samples showed few significant differences.TARDIKISS experiment, together with a previous space experiment (TARSE), further confirms that both desiccated and hydrated tardigrades represent useful animal tool for space research.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Via D. Trentacoste 2, 20134 Milano, Italy.

ABSTRACT
The TARDIKISS (Tardigrades in Space) experiment was part of the Biokon in Space (BIOKIS) payload, a set of multidisciplinary experiments performed during the DAMA (Dark Matter) mission organized by Italian Space Agency and Italian Air Force in 2011. This mission supported the execution of experiments in short duration (16 days) taking the advantage of the microgravity environment on board of the Space Shuttle Endeavour (its last mission STS-134) docked to the International Space Station. TARDIKISS was composed of three sample sets: one flight sample and two ground control samples. These samples provided the biological material used to test as space stressors, including microgravity, affected animal survivability, life cycle, DNA integrity, and pathways of molecules working as antioxidants. In this paper we compared the molecular pathways of some antioxidant molecules, thiobarbituric acid reactive substances, and fatty acid composition between flight and control samples in two tardigrade species, namely, Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. In both species, the activities of ROS scavenging enzymes, the total content of glutathione, and the fatty acids composition between flight and control samples showed few significant differences. TARDIKISS experiment, together with a previous space experiment (TARSE), further confirms that both desiccated and hydrated tardigrades represent useful animal tool for space research.

Show MeSH
Superoxide dismutase (a) and catalase (b) activities in flight and ground temperature control (TC) samples in the tardigrades Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. The bars show the mean with SD.
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fig3: Superoxide dismutase (a) and catalase (b) activities in flight and ground temperature control (TC) samples in the tardigrades Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. The bars show the mean with SD.

Mentions: In both species, the comparative analysis of the enzyme activities and other antioxidant molecules between flight (F) and temperature control samples (TC) showed few significant differences (Figures 3 and 4). In particular, a significant decrease (P < 0.05) of the glutathione reductase activity was detected in R. oberhaeuseri F samples with respect to TC samples (Figure 4(b)). Although not statistically supported, in this species a tendency to decrease catalase, superoxide dismutase, and glutathione peroxidase activity and in glutathione content was detected. In P. richtersi, a tendency to decrease catalase, superoxide dismutase, and glutathione reductase activities and to increase the glutathione peroxidase activity was detected. Noteworthy, differences were recorded in the activities of ROS scavenging enzymes between the two species.


Space flight effects on antioxidant molecules in dry tardigrades: the TARDIKISS experiment.

Rizzo AM, Altiero T, Corsetto PA, Montorfano G, Guidetti R, Rebecchi L - Biomed Res Int (2015)

Superoxide dismutase (a) and catalase (b) activities in flight and ground temperature control (TC) samples in the tardigrades Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. The bars show the mean with SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309218&req=5

fig3: Superoxide dismutase (a) and catalase (b) activities in flight and ground temperature control (TC) samples in the tardigrades Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. The bars show the mean with SD.
Mentions: In both species, the comparative analysis of the enzyme activities and other antioxidant molecules between flight (F) and temperature control samples (TC) showed few significant differences (Figures 3 and 4). In particular, a significant decrease (P < 0.05) of the glutathione reductase activity was detected in R. oberhaeuseri F samples with respect to TC samples (Figure 4(b)). Although not statistically supported, in this species a tendency to decrease catalase, superoxide dismutase, and glutathione peroxidase activity and in glutathione content was detected. In P. richtersi, a tendency to decrease catalase, superoxide dismutase, and glutathione reductase activities and to increase the glutathione peroxidase activity was detected. Noteworthy, differences were recorded in the activities of ROS scavenging enzymes between the two species.

Bottom Line: In this paper we compared the molecular pathways of some antioxidant molecules, thiobarbituric acid reactive substances, and fatty acid composition between flight and control samples in two tardigrade species, namely, Paramacrobiotus richtersi and Ramazzottius oberhaeuseri.In both species, the activities of ROS scavenging enzymes, the total content of glutathione, and the fatty acids composition between flight and control samples showed few significant differences.TARDIKISS experiment, together with a previous space experiment (TARSE), further confirms that both desiccated and hydrated tardigrades represent useful animal tool for space research.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Via D. Trentacoste 2, 20134 Milano, Italy.

ABSTRACT
The TARDIKISS (Tardigrades in Space) experiment was part of the Biokon in Space (BIOKIS) payload, a set of multidisciplinary experiments performed during the DAMA (Dark Matter) mission organized by Italian Space Agency and Italian Air Force in 2011. This mission supported the execution of experiments in short duration (16 days) taking the advantage of the microgravity environment on board of the Space Shuttle Endeavour (its last mission STS-134) docked to the International Space Station. TARDIKISS was composed of three sample sets: one flight sample and two ground control samples. These samples provided the biological material used to test as space stressors, including microgravity, affected animal survivability, life cycle, DNA integrity, and pathways of molecules working as antioxidants. In this paper we compared the molecular pathways of some antioxidant molecules, thiobarbituric acid reactive substances, and fatty acid composition between flight and control samples in two tardigrade species, namely, Paramacrobiotus richtersi and Ramazzottius oberhaeuseri. In both species, the activities of ROS scavenging enzymes, the total content of glutathione, and the fatty acids composition between flight and control samples showed few significant differences. TARDIKISS experiment, together with a previous space experiment (TARSE), further confirms that both desiccated and hydrated tardigrades represent useful animal tool for space research.

Show MeSH