Limits...
Histoarchitecture of the ovary of Rhipicephalus (Boophilus) annulatus during pre- and postengorgement period.

Kanapadinchareveetil S, Chandrasekhar L, Gopi J, Lenka DR, Vasu A, KGopalan AK, Nair SN, Ravindran R, Juliet S, Ghosh S - ScientificWorldJournal (2015)

Bottom Line: All the stages (II, III, IV, and V) of oocytes except stage I were similar to R. (B.) microplus.The stage I oocytes showed basophilia, which was not reported earlier in other species of ticks.Day wise changes were in the form of presence of oogonia in partially fed and day one engorged adults, considerable degeneration of oocytes on day two, emergence of new wave of oocytes on day three, presence of mature oocytes up to day eight, and complete degeneration of ovarian tissue from day eight onwards.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala 673 576, India.

ABSTRACT
The present communication describes the detailed day wise study of histological changes of the ovary of Rhipicephalus (Boophilus) annulatus in the postengorgement period together with the systematic classification of their oocytes. The ovary of R. (B.) annulatus is panoistic type with an asynchronous development of oocytes. All the stages (II, III, IV, and V) of oocytes except stage I were similar to R. (B.) microplus. The stage I oocytes showed basophilia, which was not reported earlier in other species of ticks. Day wise changes were in the form of presence of oogonia in partially fed and day one engorged adults, considerable degeneration of oocytes on day two, emergence of new wave of oocytes on day three, presence of mature oocytes up to day eight, and complete degeneration of ovarian tissue from day eight onwards. The degenerative changes in the ovary appeared initially in the oocytes followed by germinal epithelium.

No MeSH data available.


Related in: MedlinePlus

Histological sections of Rhipicephalus (Boophilus) annulatus ovary stained by Haematoxylin and Eosin. (a) Ovary with central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages. (b) Pedicel cells attaching the oocytes to the ovary wall. (c) Stage I oocyte. (d) Stage II oocyte. (e) Stage III oocyte. (f) Stage IV oocyte. (g) Stage V oocyte. Pe: Pedicel cell; Gv: Germ vesicle; Y: yolk droplet; Ch: chorion. Bars: (a) 4x, (b) 10x, (c)–(g) 20x.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4309210&req=5

fig1: Histological sections of Rhipicephalus (Boophilus) annulatus ovary stained by Haematoxylin and Eosin. (a) Ovary with central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages. (b) Pedicel cells attaching the oocytes to the ovary wall. (c) Stage I oocyte. (d) Stage II oocyte. (e) Stage III oocyte. (f) Stage IV oocyte. (g) Stage V oocyte. Pe: Pedicel cell; Gv: Germ vesicle; Y: yolk droplet; Ch: chorion. Bars: (a) 4x, (b) 10x, (c)–(g) 20x.

Mentions: The ovary of Rhipicephalus (Boophilus) annulatus consisted of a horseshoe shaped single continuous tubular structure located at the posterior third of the body. Similar observations were reported in Dermacentor andersoni, D. variabilis [20], Amblyomma cajennense [6], A. braziliense [10], and Rhipicephalus sanguineus [7]. The wall of adult tick ovary consisted of central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages (Figure 1(a)). The oocytes were attached to the ovary wall by specialized epithelial cells called pedicel cells [6, 7, 9, 21] (Figure 1(b)) with elongated nuclei. The oocytes were classified into stages varying from I to V (Table 1). Asynchronous development of oocytes in the ovary of R. (B.) annulatus was observed in the present study. Similar observations were reported previously [6, 7, 9, 21]. However, synchronous development was also observed in the semiengorged ticks of A. triste [8].


Histoarchitecture of the ovary of Rhipicephalus (Boophilus) annulatus during pre- and postengorgement period.

Kanapadinchareveetil S, Chandrasekhar L, Gopi J, Lenka DR, Vasu A, KGopalan AK, Nair SN, Ravindran R, Juliet S, Ghosh S - ScientificWorldJournal (2015)

Histological sections of Rhipicephalus (Boophilus) annulatus ovary stained by Haematoxylin and Eosin. (a) Ovary with central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages. (b) Pedicel cells attaching the oocytes to the ovary wall. (c) Stage I oocyte. (d) Stage II oocyte. (e) Stage III oocyte. (f) Stage IV oocyte. (g) Stage V oocyte. Pe: Pedicel cell; Gv: Germ vesicle; Y: yolk droplet; Ch: chorion. Bars: (a) 4x, (b) 10x, (c)–(g) 20x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4309210&req=5

fig1: Histological sections of Rhipicephalus (Boophilus) annulatus ovary stained by Haematoxylin and Eosin. (a) Ovary with central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages. (b) Pedicel cells attaching the oocytes to the ovary wall. (c) Stage I oocyte. (d) Stage II oocyte. (e) Stage III oocyte. (f) Stage IV oocyte. (g) Stage V oocyte. Pe: Pedicel cell; Gv: Germ vesicle; Y: yolk droplet; Ch: chorion. Bars: (a) 4x, (b) 10x, (c)–(g) 20x.
Mentions: The ovary of Rhipicephalus (Boophilus) annulatus consisted of a horseshoe shaped single continuous tubular structure located at the posterior third of the body. Similar observations were reported in Dermacentor andersoni, D. variabilis [20], Amblyomma cajennense [6], A. braziliense [10], and Rhipicephalus sanguineus [7]. The wall of adult tick ovary consisted of central lumen lined by small epithelial cells interspersed by oocytes in different developmental stages (Figure 1(a)). The oocytes were attached to the ovary wall by specialized epithelial cells called pedicel cells [6, 7, 9, 21] (Figure 1(b)) with elongated nuclei. The oocytes were classified into stages varying from I to V (Table 1). Asynchronous development of oocytes in the ovary of R. (B.) annulatus was observed in the present study. Similar observations were reported previously [6, 7, 9, 21]. However, synchronous development was also observed in the semiengorged ticks of A. triste [8].

Bottom Line: All the stages (II, III, IV, and V) of oocytes except stage I were similar to R. (B.) microplus.The stage I oocytes showed basophilia, which was not reported earlier in other species of ticks.Day wise changes were in the form of presence of oogonia in partially fed and day one engorged adults, considerable degeneration of oocytes on day two, emergence of new wave of oocytes on day three, presence of mature oocytes up to day eight, and complete degeneration of ovarian tissue from day eight onwards.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala 673 576, India.

ABSTRACT
The present communication describes the detailed day wise study of histological changes of the ovary of Rhipicephalus (Boophilus) annulatus in the postengorgement period together with the systematic classification of their oocytes. The ovary of R. (B.) annulatus is panoistic type with an asynchronous development of oocytes. All the stages (II, III, IV, and V) of oocytes except stage I were similar to R. (B.) microplus. The stage I oocytes showed basophilia, which was not reported earlier in other species of ticks. Day wise changes were in the form of presence of oogonia in partially fed and day one engorged adults, considerable degeneration of oocytes on day two, emergence of new wave of oocytes on day three, presence of mature oocytes up to day eight, and complete degeneration of ovarian tissue from day eight onwards. The degenerative changes in the ovary appeared initially in the oocytes followed by germinal epithelium.

No MeSH data available.


Related in: MedlinePlus