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Use of lanthanides to alleviate the effects of metal ion-deficiency in Desmodesmus quadricauda (Sphaeropleales, Chlorophyta).

Goecke F, Jerez CG, Zachleder V, Figueroa FL, Bišová K, Řezanka T, Vítová M - Front Microbiol (2015)

Bottom Line: We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance.In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence.These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology Academy of Sciences of the Czech Republic Třeboň, Czech Republic.

ABSTRACT
Lanthanides are biologically non-essential elements with wide applications in technology and industry. Their concentration as environmental contaminants is, therefore, increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants, even though their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements. We tested the effect of low concentrations of lanthanides on the common freshwater microalga Desmodesmus quadricauda, grown under conditions of metal ion-deficiency (lower calcium or manganese concentrations). Our goal was to test if lanthanides can replace essential metals in their functions. Physiological stress was recorded by studying growth and photosynthetic activity using a pulse amplitude modulation (PAM) fluorimeter. We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance. After adding low concentrations of five lanthanides, we confirmed that they can produce a stimulatory effect on microalgae, depending on the nutrient (metal) deprivation. In the case of a calcium deficit, the addition of lanthanides partly alleviated the adverse effects, probably by a partial substitution of the element. In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence. These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

No MeSH data available.


Related in: MedlinePlus

Changes in dry weight in cultures of the alga Desmodesmus quadricauda grown either in complete mineral medium (Ctrl, red symbols, dashed curve) or in calcium- (upper raw of panels) or manganese-deficient mineral medium (bottom raw of panels) (Def., blue symbols, dashed curves). To calcium and manganese deficient cultures either the complete mineral medium was added (Rec, black symbols, solid line) or different lanthanides (Ce, Eu, Gd, La, Nd) as marked in individual panels. The curves (without symbols) from recovered (Rec) and deficient (Def) cultures are inserted in panels illustrating the growth in the presence of lanthanides. Supplementary information see Figure S1.
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Figure 2: Changes in dry weight in cultures of the alga Desmodesmus quadricauda grown either in complete mineral medium (Ctrl, red symbols, dashed curve) or in calcium- (upper raw of panels) or manganese-deficient mineral medium (bottom raw of panels) (Def., blue symbols, dashed curves). To calcium and manganese deficient cultures either the complete mineral medium was added (Rec, black symbols, solid line) or different lanthanides (Ce, Eu, Gd, La, Nd) as marked in individual panels. The curves (without symbols) from recovered (Rec) and deficient (Def) cultures are inserted in panels illustrating the growth in the presence of lanthanides. Supplementary information see Figure S1.

Mentions: The growth of D. quadricauda under complete mineral medium as a control (Ctrl), or under calcium- or manganese-deficient mineral medium is shown in Figure 2. The control and deprived conditions are graphically represented with the red and blue lines, respectively. Our results showed that a deficiency in either metal (independently), but especially Mn2+, significantly decreased cellular growth of the microalga (p < 0.05) (as “Def” in Figure 2). Re-establishment of the standard medium resulted in recovery of growth in the metal-deprived strain (as “Rec” in Figure 2).


Use of lanthanides to alleviate the effects of metal ion-deficiency in Desmodesmus quadricauda (Sphaeropleales, Chlorophyta).

Goecke F, Jerez CG, Zachleder V, Figueroa FL, Bišová K, Řezanka T, Vítová M - Front Microbiol (2015)

Changes in dry weight in cultures of the alga Desmodesmus quadricauda grown either in complete mineral medium (Ctrl, red symbols, dashed curve) or in calcium- (upper raw of panels) or manganese-deficient mineral medium (bottom raw of panels) (Def., blue symbols, dashed curves). To calcium and manganese deficient cultures either the complete mineral medium was added (Rec, black symbols, solid line) or different lanthanides (Ce, Eu, Gd, La, Nd) as marked in individual panels. The curves (without symbols) from recovered (Rec) and deficient (Def) cultures are inserted in panels illustrating the growth in the presence of lanthanides. Supplementary information see Figure S1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309186&req=5

Figure 2: Changes in dry weight in cultures of the alga Desmodesmus quadricauda grown either in complete mineral medium (Ctrl, red symbols, dashed curve) or in calcium- (upper raw of panels) or manganese-deficient mineral medium (bottom raw of panels) (Def., blue symbols, dashed curves). To calcium and manganese deficient cultures either the complete mineral medium was added (Rec, black symbols, solid line) or different lanthanides (Ce, Eu, Gd, La, Nd) as marked in individual panels. The curves (without symbols) from recovered (Rec) and deficient (Def) cultures are inserted in panels illustrating the growth in the presence of lanthanides. Supplementary information see Figure S1.
Mentions: The growth of D. quadricauda under complete mineral medium as a control (Ctrl), or under calcium- or manganese-deficient mineral medium is shown in Figure 2. The control and deprived conditions are graphically represented with the red and blue lines, respectively. Our results showed that a deficiency in either metal (independently), but especially Mn2+, significantly decreased cellular growth of the microalga (p < 0.05) (as “Def” in Figure 2). Re-establishment of the standard medium resulted in recovery of growth in the metal-deprived strain (as “Rec” in Figure 2).

Bottom Line: We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance.In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence.These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology Academy of Sciences of the Czech Republic Třeboň, Czech Republic.

ABSTRACT
Lanthanides are biologically non-essential elements with wide applications in technology and industry. Their concentration as environmental contaminants is, therefore, increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants, even though their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements. We tested the effect of low concentrations of lanthanides on the common freshwater microalga Desmodesmus quadricauda, grown under conditions of metal ion-deficiency (lower calcium or manganese concentrations). Our goal was to test if lanthanides can replace essential metals in their functions. Physiological stress was recorded by studying growth and photosynthetic activity using a pulse amplitude modulation (PAM) fluorimeter. We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance. After adding low concentrations of five lanthanides, we confirmed that they can produce a stimulatory effect on microalgae, depending on the nutrient (metal) deprivation. In the case of a calcium deficit, the addition of lanthanides partly alleviated the adverse effects, probably by a partial substitution of the element. In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence. These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

No MeSH data available.


Related in: MedlinePlus