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Use of lanthanides to alleviate the effects of metal ion-deficiency in Desmodesmus quadricauda (Sphaeropleales, Chlorophyta).

Goecke F, Jerez CG, Zachleder V, Figueroa FL, Bišová K, Řezanka T, Vítová M - Front Microbiol (2015)

Bottom Line: We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance.In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence.These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology Academy of Sciences of the Czech Republic Třeboň, Czech Republic.

ABSTRACT
Lanthanides are biologically non-essential elements with wide applications in technology and industry. Their concentration as environmental contaminants is, therefore, increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants, even though their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements. We tested the effect of low concentrations of lanthanides on the common freshwater microalga Desmodesmus quadricauda, grown under conditions of metal ion-deficiency (lower calcium or manganese concentrations). Our goal was to test if lanthanides can replace essential metals in their functions. Physiological stress was recorded by studying growth and photosynthetic activity using a pulse amplitude modulation (PAM) fluorimeter. We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance. After adding low concentrations of five lanthanides, we confirmed that they can produce a stimulatory effect on microalgae, depending on the nutrient (metal) deprivation. In the case of a calcium deficit, the addition of lanthanides partly alleviated the adverse effects, probably by a partial substitution of the element. In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence. These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

No MeSH data available.


Related in: MedlinePlus

Changes in cell number in cultures of the alga Desmodesmus quadricauda grown in the presence of different concentration of cerium. The suspensions of cultures were grown in a 96-well microplate for 3 days.
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Figure 1: Changes in cell number in cultures of the alga Desmodesmus quadricauda grown in the presence of different concentration of cerium. The suspensions of cultures were grown in a 96-well microplate for 3 days.

Mentions: Specific growth rates in the preliminary experiment (Table 3) were determined from the slopes of linear regressions of the natural log of cell concentration vs. time for the data plotted in Figure 1.


Use of lanthanides to alleviate the effects of metal ion-deficiency in Desmodesmus quadricauda (Sphaeropleales, Chlorophyta).

Goecke F, Jerez CG, Zachleder V, Figueroa FL, Bišová K, Řezanka T, Vítová M - Front Microbiol (2015)

Changes in cell number in cultures of the alga Desmodesmus quadricauda grown in the presence of different concentration of cerium. The suspensions of cultures were grown in a 96-well microplate for 3 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309186&req=5

Figure 1: Changes in cell number in cultures of the alga Desmodesmus quadricauda grown in the presence of different concentration of cerium. The suspensions of cultures were grown in a 96-well microplate for 3 days.
Mentions: Specific growth rates in the preliminary experiment (Table 3) were determined from the slopes of linear regressions of the natural log of cell concentration vs. time for the data plotted in Figure 1.

Bottom Line: We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance.In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence.These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology Academy of Sciences of the Czech Republic Třeboň, Czech Republic.

ABSTRACT
Lanthanides are biologically non-essential elements with wide applications in technology and industry. Their concentration as environmental contaminants is, therefore, increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants, even though their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements. We tested the effect of low concentrations of lanthanides on the common freshwater microalga Desmodesmus quadricauda, grown under conditions of metal ion-deficiency (lower calcium or manganese concentrations). Our goal was to test if lanthanides can replace essential metals in their functions. Physiological stress was recorded by studying growth and photosynthetic activity using a pulse amplitude modulation (PAM) fluorimeter. We found that nutrient stress reduced parameters of growth and photosynthesis, such as maximal quantum yield, relative electron transport rate, photon capturing efficiency and light saturation irradiance. After adding low concentrations of five lanthanides, we confirmed that they can produce a stimulatory effect on microalgae, depending on the nutrient (metal) deprivation. In the case of a calcium deficit, the addition of lanthanides partly alleviated the adverse effects, probably by a partial substitution of the element. In contrast, with manganese deprivation (and at even lower concentrations), lanthanides enhanced the deleterious effect on cellular growth and photosynthetic competence. These results show that lanthanides can replace essential elements, but their effects on microalgae depend on stress and the nutritional state of the microalgae, raising the possibility of environmental impacts at even low concentrations.

No MeSH data available.


Related in: MedlinePlus