Limits...
Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.


Related in: MedlinePlus

Invertebrate abundance in relation to the expected heterozygosity (He) measured by SSR markers. Data were fitted by linear regression. The abundance of arthropods was determined for each plot. Open symbols: plots containing only P. tremula demes, closed symbols: plots containing P. tremuloides demes. Inset: Beetles abundance in 1-, 2-, and 4-deme plots with P. tremula. Data are shown as box plots indicating the 10-, 25, 50, and 90% range of the data. In the boxes the vertical line indicates the median and black circle the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309117&req=5

Figure 5: Invertebrate abundance in relation to the expected heterozygosity (He) measured by SSR markers. Data were fitted by linear regression. The abundance of arthropods was determined for each plot. Open symbols: plots containing only P. tremula demes, closed symbols: plots containing P. tremuloides demes. Inset: Beetles abundance in 1-, 2-, and 4-deme plots with P. tremula. Data are shown as box plots indicating the 10-, 25, 50, and 90% range of the data. In the boxes the vertical line indicates the median and black circle the mean.

Mentions: To investigate potential relationships between the genetic diversity and the abundance of invertebrates we correlated expected heterozygosity (He) with invertebrate abundance. Since our previous analysis showed a strong effect of P. tremuloides on the genetic diversity indices, rank correlation analyses were conducted for the detected invertebrate groups for all plots and additionally only for the plots without the USA deme (Table 7). When all plots were included we found that He was significantly correlated with beetle abundance (positive) and with caterpillar abundance (negative; Table 7). The relationship between He and total invertebrate abundance was also significant and positive (Figure 5). However, when plots with and without P. tremuloides were analyzed separately, it was apparent that invertebrate abundance was unrelated to P. tremula diversity and that the observed increase was driven by the presence of P. tremuloides (Figure 5). When the correlation analysis was conducted with the Shannon indices of the AFLP markers for all plots, the relationship with invertebrate abundance was also highly significant (R = 0.35, P < 0.001) and disappeared when P. tremuloides was removed from the analysis. Correlations were neither detected when the invertebrate groups (spiders, beetles, caterpillars, and flies) in plots with and without P. tremuloides were analyzed separately (Table 7).


Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Invertebrate abundance in relation to the expected heterozygosity (He) measured by SSR markers. Data were fitted by linear regression. The abundance of arthropods was determined for each plot. Open symbols: plots containing only P. tremula demes, closed symbols: plots containing P. tremuloides demes. Inset: Beetles abundance in 1-, 2-, and 4-deme plots with P. tremula. Data are shown as box plots indicating the 10-, 25, 50, and 90% range of the data. In the boxes the vertical line indicates the median and black circle the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309117&req=5

Figure 5: Invertebrate abundance in relation to the expected heterozygosity (He) measured by SSR markers. Data were fitted by linear regression. The abundance of arthropods was determined for each plot. Open symbols: plots containing only P. tremula demes, closed symbols: plots containing P. tremuloides demes. Inset: Beetles abundance in 1-, 2-, and 4-deme plots with P. tremula. Data are shown as box plots indicating the 10-, 25, 50, and 90% range of the data. In the boxes the vertical line indicates the median and black circle the mean.
Mentions: To investigate potential relationships between the genetic diversity and the abundance of invertebrates we correlated expected heterozygosity (He) with invertebrate abundance. Since our previous analysis showed a strong effect of P. tremuloides on the genetic diversity indices, rank correlation analyses were conducted for the detected invertebrate groups for all plots and additionally only for the plots without the USA deme (Table 7). When all plots were included we found that He was significantly correlated with beetle abundance (positive) and with caterpillar abundance (negative; Table 7). The relationship between He and total invertebrate abundance was also significant and positive (Figure 5). However, when plots with and without P. tremuloides were analyzed separately, it was apparent that invertebrate abundance was unrelated to P. tremula diversity and that the observed increase was driven by the presence of P. tremuloides (Figure 5). When the correlation analysis was conducted with the Shannon indices of the AFLP markers for all plots, the relationship with invertebrate abundance was also highly significant (R = 0.35, P < 0.001) and disappeared when P. tremuloides was removed from the analysis. Correlations were neither detected when the invertebrate groups (spiders, beetles, caterpillars, and flies) in plots with and without P. tremuloides were analyzed separately (Table 7).

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.


Related in: MedlinePlus