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Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.


Unweighted pair group method with arithmetic mean dendrograms based on genetic distances calculated from both SSRs and AFLPs. (A)Nei’s (1972) genetic distances based on SSRs between demes was used to construct the UPGMA dendrogram. (B) Consensus UPGMA dendrogram was drawn based on genetic dissimilarity between demes (genetic similarity based on simple matching coefficient) calculated from AFLPs. Numbers at nodes are percentage over 1000 bootstrap replicates. Abbreviations for the demes are as in Table 1.
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Figure 3: Unweighted pair group method with arithmetic mean dendrograms based on genetic distances calculated from both SSRs and AFLPs. (A)Nei’s (1972) genetic distances based on SSRs between demes was used to construct the UPGMA dendrogram. (B) Consensus UPGMA dendrogram was drawn based on genetic dissimilarity between demes (genetic similarity based on simple matching coefficient) calculated from AFLPs. Numbers at nodes are percentage over 1000 bootstrap replicates. Abbreviations for the demes are as in Table 1.

Mentions: For both SSR and AFLP markers, the genetic distances between the eight demes were visualized in UPGMA dendrograms (Figure 3). Congruent results were obtained from both markers. Large genetic distances were observed between the North American P. tremuloides deme and the European P. tremula demes resulting in clear outgrouping of the North American P. tremuloides deme from the European P. tremula demes with high bootstrap support (89% at SSRs and 100% at AFLPs). This finding confirmed that P. tremuloides represents a genetically distinct species in comparison to the European P. tremula demes.


Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Unweighted pair group method with arithmetic mean dendrograms based on genetic distances calculated from both SSRs and AFLPs. (A)Nei’s (1972) genetic distances based on SSRs between demes was used to construct the UPGMA dendrogram. (B) Consensus UPGMA dendrogram was drawn based on genetic dissimilarity between demes (genetic similarity based on simple matching coefficient) calculated from AFLPs. Numbers at nodes are percentage over 1000 bootstrap replicates. Abbreviations for the demes are as in Table 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309117&req=5

Figure 3: Unweighted pair group method with arithmetic mean dendrograms based on genetic distances calculated from both SSRs and AFLPs. (A)Nei’s (1972) genetic distances based on SSRs between demes was used to construct the UPGMA dendrogram. (B) Consensus UPGMA dendrogram was drawn based on genetic dissimilarity between demes (genetic similarity based on simple matching coefficient) calculated from AFLPs. Numbers at nodes are percentage over 1000 bootstrap replicates. Abbreviations for the demes are as in Table 1.
Mentions: For both SSR and AFLP markers, the genetic distances between the eight demes were visualized in UPGMA dendrograms (Figure 3). Congruent results were obtained from both markers. Large genetic distances were observed between the North American P. tremuloides deme and the European P. tremula demes resulting in clear outgrouping of the North American P. tremuloides deme from the European P. tremula demes with high bootstrap support (89% at SSRs and 100% at AFLPs). This finding confirmed that P. tremuloides represents a genetically distinct species in comparison to the European P. tremula demes.

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.