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Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.


Correlation of genetic diversity estimates from SSRs (expected heterozygosity) and AFLPs (Shannon index) across eight aspen demes. For the Swedish deme, diversity estimates were used for Pearson correlation analysis after removing the clonal replicates (see Table 3 S–, for details).
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Figure 2: Correlation of genetic diversity estimates from SSRs (expected heterozygosity) and AFLPs (Shannon index) across eight aspen demes. For the Swedish deme, diversity estimates were used for Pearson correlation analysis after removing the clonal replicates (see Table 3 S–, for details).

Mentions: The two AFLP primer pair combinations yielded 163 clear and repeatable peaks in the range of 75–492 bp. By far most private alleles were present within the North American P. tremuloides deme. The observation of the maximum genetic diversity within the American P. tremuloides deme and the minimum genetic diversity within deme G8 was confirmed at the AFLP markers based on all analyzed parameters including the number of polymorphic loci and Shannon indices (Table 3). The Pearson correlation between the expected heterozygosity at SSR markers and Shannon indices at AFLPs was highly significant (R = 0.906, P < 0.005, Figure 2).


Genetic diversity in aspen and its relation to arthropod abundance.

Zhang C, Vornam B, Volmer K, Prinz K, Kleemann F, Köhler L, Polle A, Finkeldey R - Front Plant Sci (2015)

Correlation of genetic diversity estimates from SSRs (expected heterozygosity) and AFLPs (Shannon index) across eight aspen demes. For the Swedish deme, diversity estimates were used for Pearson correlation analysis after removing the clonal replicates (see Table 3 S–, for details).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309117&req=5

Figure 2: Correlation of genetic diversity estimates from SSRs (expected heterozygosity) and AFLPs (Shannon index) across eight aspen demes. For the Swedish deme, diversity estimates were used for Pearson correlation analysis after removing the clonal replicates (see Table 3 S–, for details).
Mentions: The two AFLP primer pair combinations yielded 163 clear and repeatable peaks in the range of 75–492 bp. By far most private alleles were present within the North American P. tremuloides deme. The observation of the maximum genetic diversity within the American P. tremuloides deme and the minimum genetic diversity within deme G8 was confirmed at the AFLP markers based on all analyzed parameters including the number of polymorphic loci and Shannon indices (Table 3). The Pearson correlation between the expected heterozygosity at SSR markers and Shannon indices at AFLPs was highly significant (R = 0.906, P < 0.005, Figure 2).

Bottom Line: Little is known on the effect of genetic diversity on ecosystem services.An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high.The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Northwest A&F University Shaanxi, China ; Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-Universität Göttingen Göttingen, Germany.

ABSTRACT
The ecological consequences of biodiversity have become a prominent public issue. Little is known on the effect of genetic diversity on ecosystem services. Here, a diversity experiment was established with European and North American aspen (Populus tremula, P. tremuloides) planted in plots representing either a single deme only or combinations of two, four and eight demes. The goals of this study were to explore the complex inter- and intraspecific genetic diversity of aspen and to then relate three measures for diversity (deme diversity, genetic diversity determined as Shannon index or as expected heterozygosity) to arthropod abundance. Microsatellite and AFLP markers were used to analyze the genetic variation patterns within and between the aspen demes and deme mixtures. Large differences were observed regarding the genetic diversity within demes. An analysis of molecular variance revealed that most of the total genetic diversity was found within demes, but the genetic differentiation among demes was also high. The complex patterns of genetic diversity and differentiation resulted in large differences of the genetic variation within plots. The average diversity increased from plots with only one deme to plots with two, four, and eight demes, respectively and separated plots with and without American aspen. To test whether intra- and interspecific diversity impacts on ecosystem services, arthropod abundance was determined. Increasing genetic diversity of aspen was related to increasing abundance of arthropods. However, the relationship was mainly driven by the presence of American aspen suggesting that species identity overrode the effect of intraspecific variation of European aspen.

No MeSH data available.