Limits...
Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus

Modulation of genes encoding enzymes involved in lignin biosynthetic pathway by boron (B)-deficiency stress. Red boxes indicate genes up-regulated only in TO by B deficient stress, green boxes indicate genes down-regulated in CC and up-regulated in TO by B deficient stress, blue boxes indicate genes up-regulated both in CC and TO by B deficient stress, and gray boxes indicate genes not significantly modulated by B-deficiency stress. The dotted line means that some steps are not shown. PAL, Phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CCR, Cinnamoyl-CoA reductase; CAD, cinnamyl alcohol dehydrogenase; POD, peroxidase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309116&req=5

Figure 6: Modulation of genes encoding enzymes involved in lignin biosynthetic pathway by boron (B)-deficiency stress. Red boxes indicate genes up-regulated only in TO by B deficient stress, green boxes indicate genes down-regulated in CC and up-regulated in TO by B deficient stress, blue boxes indicate genes up-regulated both in CC and TO by B deficient stress, and gray boxes indicate genes not significantly modulated by B-deficiency stress. The dotted line means that some steps are not shown. PAL, Phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CCR, Cinnamoyl-CoA reductase; CAD, cinnamyl alcohol dehydrogenase; POD, peroxidase.

Mentions: Four genes encoding key enzymes in the lignin biosynthesis pathway were significantly up-regulated under B-deficiency (Table 3 and Figure 6). These genes include phenylalanine ammonia-lyase (PAL; JK817683), 4-coumarate: CoA ligase (4CL; JK817661), cinnamoyl-CoA reductase4 (CCR4; KJ817664) and peroxidase (POD; JK817640 and JK817712). All these genes were up-regulated in TO plants root under B-deficiency at all time points, while only POD in CC plants root after 24 h B-deficiency treatment. In order to further investigate the different morphology of root cell wall induced by the change of lignin in the cell wall between CC and TO, histochemical staining and microscopy were performed. As shown in Figures 7A–D, the root sections were stained histochemically stained with phloroglucinol. According to the color intensity, which approximately reflects the total lignin content, the lignin quantity in the root cell walls of TO was much higher than CC under B-deficiency conditions (Figures 7B,D). Electron microscopy analysis demonstrated that the root of TO also showed heavily thickened cell walls (Figure 7H) and a thickened folded cell wall structure (Figure 7L) under B-deficiency conditions, compared with that of the control (Figures 7G,K). However, only a slight thickened cell walls were observed in CC (Figures 7E,F,I,J).


Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

Modulation of genes encoding enzymes involved in lignin biosynthetic pathway by boron (B)-deficiency stress. Red boxes indicate genes up-regulated only in TO by B deficient stress, green boxes indicate genes down-regulated in CC and up-regulated in TO by B deficient stress, blue boxes indicate genes up-regulated both in CC and TO by B deficient stress, and gray boxes indicate genes not significantly modulated by B-deficiency stress. The dotted line means that some steps are not shown. PAL, Phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CCR, Cinnamoyl-CoA reductase; CAD, cinnamyl alcohol dehydrogenase; POD, peroxidase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309116&req=5

Figure 6: Modulation of genes encoding enzymes involved in lignin biosynthetic pathway by boron (B)-deficiency stress. Red boxes indicate genes up-regulated only in TO by B deficient stress, green boxes indicate genes down-regulated in CC and up-regulated in TO by B deficient stress, blue boxes indicate genes up-regulated both in CC and TO by B deficient stress, and gray boxes indicate genes not significantly modulated by B-deficiency stress. The dotted line means that some steps are not shown. PAL, Phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CCR, Cinnamoyl-CoA reductase; CAD, cinnamyl alcohol dehydrogenase; POD, peroxidase.
Mentions: Four genes encoding key enzymes in the lignin biosynthesis pathway were significantly up-regulated under B-deficiency (Table 3 and Figure 6). These genes include phenylalanine ammonia-lyase (PAL; JK817683), 4-coumarate: CoA ligase (4CL; JK817661), cinnamoyl-CoA reductase4 (CCR4; KJ817664) and peroxidase (POD; JK817640 and JK817712). All these genes were up-regulated in TO plants root under B-deficiency at all time points, while only POD in CC plants root after 24 h B-deficiency treatment. In order to further investigate the different morphology of root cell wall induced by the change of lignin in the cell wall between CC and TO, histochemical staining and microscopy were performed. As shown in Figures 7A–D, the root sections were stained histochemically stained with phloroglucinol. According to the color intensity, which approximately reflects the total lignin content, the lignin quantity in the root cell walls of TO was much higher than CC under B-deficiency conditions (Figures 7B,D). Electron microscopy analysis demonstrated that the root of TO also showed heavily thickened cell walls (Figure 7H) and a thickened folded cell wall structure (Figure 7L) under B-deficiency conditions, compared with that of the control (Figures 7G,K). However, only a slight thickened cell walls were observed in CC (Figures 7E,F,I,J).

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus