Limits...
Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus

Phenotypic responses of Carrizo citrange and Trifoliate orange root by boron-deficient stress. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. After that root dry weight (A) and B concentration in root (B) were measured. Moreover, the root morphologies of root total length (C), root surface (D), root volume (E) and root number (F) were also measured. Data are presented as mean ± SE of three biological replicates (n = 6), samples from six plants were collected for each biological replicate. Different letters indicate significant differences (p < 0.05) between the different genotypes and different treatments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309116&req=5

Figure 2: Phenotypic responses of Carrizo citrange and Trifoliate orange root by boron-deficient stress. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. After that root dry weight (A) and B concentration in root (B) were measured. Moreover, the root morphologies of root total length (C), root surface (D), root volume (E) and root number (F) were also measured. Data are presented as mean ± SE of three biological replicates (n = 6), samples from six plants were collected for each biological replicate. Different letters indicate significant differences (p < 0.05) between the different genotypes and different treatments.

Mentions: The root dry weight was dramatically decreased in TO, but only a small decrease was observed in CC under B-deficiency when compared to control (Figures 1A,B, 2A). In addition, B concentration in the root of TO was significantly reduced under B-deficiency treatment, whereas no effect was found in the root of CC. It is worthy to note that the concentration in the root of CC was lower than in TO under normal conditions (Figure 2B). The root morphology of these two rootstocks was also analyzed under B-deficiency conditions. The root length, root surface area, root volume and root number were decreased markedly in response to B-deficient stress in TO. However, only the root volume was decreased significantly in CC under B-deficient conditions and no significant decrease was found for other three parameters (Figures 2C–F). All these results supported that CC was more tolerant to B-deficiency than TO.


Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

Phenotypic responses of Carrizo citrange and Trifoliate orange root by boron-deficient stress. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. After that root dry weight (A) and B concentration in root (B) were measured. Moreover, the root morphologies of root total length (C), root surface (D), root volume (E) and root number (F) were also measured. Data are presented as mean ± SE of three biological replicates (n = 6), samples from six plants were collected for each biological replicate. Different letters indicate significant differences (p < 0.05) between the different genotypes and different treatments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309116&req=5

Figure 2: Phenotypic responses of Carrizo citrange and Trifoliate orange root by boron-deficient stress. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. After that root dry weight (A) and B concentration in root (B) were measured. Moreover, the root morphologies of root total length (C), root surface (D), root volume (E) and root number (F) were also measured. Data are presented as mean ± SE of three biological replicates (n = 6), samples from six plants were collected for each biological replicate. Different letters indicate significant differences (p < 0.05) between the different genotypes and different treatments.
Mentions: The root dry weight was dramatically decreased in TO, but only a small decrease was observed in CC under B-deficiency when compared to control (Figures 1A,B, 2A). In addition, B concentration in the root of TO was significantly reduced under B-deficiency treatment, whereas no effect was found in the root of CC. It is worthy to note that the concentration in the root of CC was lower than in TO under normal conditions (Figure 2B). The root morphology of these two rootstocks was also analyzed under B-deficiency conditions. The root length, root surface area, root volume and root number were decreased markedly in response to B-deficient stress in TO. However, only the root volume was decreased significantly in CC under B-deficient conditions and no significant decrease was found for other three parameters (Figures 2C–F). All these results supported that CC was more tolerant to B-deficiency than TO.

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus