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Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus

The different performance of Carrizo citrange (CC) and Trifoliate orange (TO) seedlings under boron (B)-deficiency stress for 8 weeks. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. (A) Root of CC under B-deficiency stress (left) and control (right); (B) Root of TO under B-deficiency stress (left) and control (right); (C) Stem of CC under B-deficiency stress (left) and control (right); (D) Stem of TO under B-deficiency stress (left) and control (right); (E) Leaf of CC under B-deficiency stress (left) and control (right); (F) Leaf of TO under B-deficiency stress (left) and control (right).
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Figure 1: The different performance of Carrizo citrange (CC) and Trifoliate orange (TO) seedlings under boron (B)-deficiency stress for 8 weeks. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. (A) Root of CC under B-deficiency stress (left) and control (right); (B) Root of TO under B-deficiency stress (left) and control (right); (C) Stem of CC under B-deficiency stress (left) and control (right); (D) Stem of TO under B-deficiency stress (left) and control (right); (E) Leaf of CC under B-deficiency stress (left) and control (right); (F) Leaf of TO under B-deficiency stress (left) and control (right).

Mentions: After 0.01 mg L−1 B treatment for 8 weeks, differential performance of CC and TO was observed in different plant parts. Vein swelling or cracking was observed in the leaves of TO under B-deficiency conditions, whereas no significant visual symptoms were detected in CC, except for a slight yellowing found in several leaves at the end of the experiment (Figures 1E,F). Due to the shoot tip necrosis, the length of stem was decreased markedly in both CC and TO under B-deficiency conditions (Figures 1C,D). The most dramatic morphological difference was found in root of TO between normal and B-deficiency treatment. The lateral roots of TO were longer under normal conditions, but they were shorter and thicker under B-deficiency conditions (Figure 1B); However, no significant difference was found in CC (Figure 1A).


Transcription profiles of boron-deficiency-responsive genes in citrus rootstock root by suppression subtractive hybridization and cDNA microarray.

Zhou GF, Liu YZ, Sheng O, Wei QJ, Yang CQ, Peng SA - Front Plant Sci (2015)

The different performance of Carrizo citrange (CC) and Trifoliate orange (TO) seedlings under boron (B)-deficiency stress for 8 weeks. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. (A) Root of CC under B-deficiency stress (left) and control (right); (B) Root of TO under B-deficiency stress (left) and control (right); (C) Stem of CC under B-deficiency stress (left) and control (right); (D) Stem of TO under B-deficiency stress (left) and control (right); (E) Leaf of CC under B-deficiency stress (left) and control (right); (F) Leaf of TO under B-deficiency stress (left) and control (right).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309116&req=5

Figure 1: The different performance of Carrizo citrange (CC) and Trifoliate orange (TO) seedlings under boron (B)-deficiency stress for 8 weeks. 2-month-old plants were grown in hydroponics and treated for another 8 weeks. (A) Root of CC under B-deficiency stress (left) and control (right); (B) Root of TO under B-deficiency stress (left) and control (right); (C) Stem of CC under B-deficiency stress (left) and control (right); (D) Stem of TO under B-deficiency stress (left) and control (right); (E) Leaf of CC under B-deficiency stress (left) and control (right); (F) Leaf of TO under B-deficiency stress (left) and control (right).
Mentions: After 0.01 mg L−1 B treatment for 8 weeks, differential performance of CC and TO was observed in different plant parts. Vein swelling or cracking was observed in the leaves of TO under B-deficiency conditions, whereas no significant visual symptoms were detected in CC, except for a slight yellowing found in several leaves at the end of the experiment (Figures 1E,F). Due to the shoot tip necrosis, the length of stem was decreased markedly in both CC and TO under B-deficiency conditions (Figures 1C,D). The most dramatic morphological difference was found in root of TO between normal and B-deficiency treatment. The lateral roots of TO were longer under normal conditions, but they were shorter and thicker under B-deficiency conditions (Figure 1B); However, no significant difference was found in CC (Figure 1A).

Bottom Line: Boron (B) deficiency has seriously negative effect on citrus production.All these results indicated that CC was more tolerant than TO to B-deficiency stress.The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University Wuhan, China ; National Navel Orange Engineering Research Center, College of Navel Orange, Gannan Normal University Ganzhou, China.

ABSTRACT
Boron (B) deficiency has seriously negative effect on citrus production. Carrizo citrange (CC) has been reported as a B-deficiency tolerant rootstock. However, the molecular mechanism of its B-deficiency tolerance remained not well-explored. To understand the molecular basis of citrus rootstock to B-deficiency, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes responsive to B-deficiency. Firstly four SSH libraries were constructed for the root tissue of two citrus rootstocks CC and Trifoliate orange (TO) to compare B-deficiency treated and non-treated plants. Then 7680 clones from these SSH libraries were used to construct a cDNA array and microarray analysis was carried out to verify the expression changes of these clones upon B-deficiency treatment at various time points compared to the corresponding controls. A total of 139 unigenes that were differentially expressed upon B-deficiency stress either in CC or TO were identified from microarray analysis, some of these genes have not previously been reported to be associated with B-deficiency stress. In this work, several genes involved in cell wall metabolism and transmembrane transport were identified to be highly regulated under B-deficiency stress, and a total of 23 metabolic pathways were affected by B-deficiency, especially the lignin biosynthesis pathway, nitrogen metabolism, and glycolytic pathway. All these results indicated that CC was more tolerant than TO to B-deficiency stress. The B-deficiency responsive genes identified in this study could provide further information for understanding the mechanisms of B-deficiency tolerance in citrus.

No MeSH data available.


Related in: MedlinePlus