Vulnerability of the developing heart to oxygen deprivation as a cause of congenital heart defects.
Bottom Line: The heart develops under reduced and varying oxygen concentrations, yet there is little understanding of oxygen metabolism in the normal and mal-development of the heart.ODD-Luc activity decreased in 2 stages, the first corresponding with the formation of a functional cardiovascular system for oxygen delivery at E15.5, and the second after birth consistent with complete oxygenation of the blood and tissues.Low oxygen concentrations and lack of oxygen reserve during a critical phase of heart organogenesis may provide a basis for vulnerability to the development of common septation and conotruncal heart defects.
Affiliation: Department of Cardiovascular Medicine, University of Maryland School of Medicine, Baltimore, MD (D.K., S.A.F.).Show MeSH
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Mentions: Hif‐1αf/f mice were crossed with Rosa26‐LacZ mice to fate map NCCs migrating into the OFT using LacZ staining. In a Hif‐1αf/f; Wnt1Cre+ embryo with PTA, LacZ+ cells populate the great vessels and OFT, including the defective aortic‐pulmonic septum (Figures 6C and 6D; compare to control 6A and 6B). This suggests that HIF‐1α is not required within the NCCs for their migration into the cardiac OFT.
Affiliation: Department of Cardiovascular Medicine, University of Maryland School of Medicine, Baltimore, MD (D.K., S.A.F.).