Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.
Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.
Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).Show MeSH
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Mentions: To evaluate antiâinflammatory and âfibrotic potential of QQMNCTx, fibrotic area in ischemic ATM was detected by Azan staining on day 21 after treatment. The mean fibrotic area (% fibrotic area/Ă40 HPF) for the QQMNCTx, PBMNCTx, and control groups were 2.78Â±0.61, 8.41Â±1.51, and 11.94Â±3.59. These findings indicate that QQMNCTx exerted greater antifibrotic effects than did PBMNCTx (Figure 9A and 9B).
Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).