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Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

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Histological evaluation of fibrosis in ischemic hindlimbs. A and C, Representative pictures of fibrosis in ATM assessed by Azan staining (blue) in each group. ×40 HPF. (a) Contralateral hindlimb of IMDM control (b, c, and d) and ischemic hindlimbs of IMDM control, PBMNCTx, and QQMNCTx in (A). (a, b, c, and d) Ischemic hindlimbs of IMDM control, eEPCTx, QQMNCTx, and GmCD34Tx in (C). B and D, The graphs show percent (%) fibrotic area in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B and D). ##P<0.01 versus PBMNCTx in (B). #P<0.05 versus eEPCTx in (D). Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
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fig09: Histological evaluation of fibrosis in ischemic hindlimbs. A and C, Representative pictures of fibrosis in ATM assessed by Azan staining (blue) in each group. ×40 HPF. (a) Contralateral hindlimb of IMDM control (b, c, and d) and ischemic hindlimbs of IMDM control, PBMNCTx, and QQMNCTx in (A). (a, b, c, and d) Ischemic hindlimbs of IMDM control, eEPCTx, QQMNCTx, and GmCD34Tx in (C). B and D, The graphs show percent (%) fibrotic area in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B and D). ##P<0.01 versus PBMNCTx in (B). #P<0.05 versus eEPCTx in (D). Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.

Mentions: To evaluate anti‐inflammatory and ‐fibrotic potential of QQMNCTx, fibrotic area in ischemic ATM was detected by Azan staining on day 21 after treatment. The mean fibrotic area (% fibrotic area/×40 HPF) for the QQMNCTx, PBMNCTx, and control groups were 2.78±0.61, 8.41±1.51, and 11.94±3.59. These findings indicate that QQMNCTx exerted greater antifibrotic effects than did PBMNCTx (Figure 9A and 9B).


Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Histological evaluation of fibrosis in ischemic hindlimbs. A and C, Representative pictures of fibrosis in ATM assessed by Azan staining (blue) in each group. ×40 HPF. (a) Contralateral hindlimb of IMDM control (b, c, and d) and ischemic hindlimbs of IMDM control, PBMNCTx, and QQMNCTx in (A). (a, b, c, and d) Ischemic hindlimbs of IMDM control, eEPCTx, QQMNCTx, and GmCD34Tx in (C). B and D, The graphs show percent (%) fibrotic area in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B and D). ##P<0.01 versus PBMNCTx in (B). #P<0.05 versus eEPCTx in (D). Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309104&req=5

fig09: Histological evaluation of fibrosis in ischemic hindlimbs. A and C, Representative pictures of fibrosis in ATM assessed by Azan staining (blue) in each group. ×40 HPF. (a) Contralateral hindlimb of IMDM control (b, c, and d) and ischemic hindlimbs of IMDM control, PBMNCTx, and QQMNCTx in (A). (a, b, c, and d) Ischemic hindlimbs of IMDM control, eEPCTx, QQMNCTx, and GmCD34Tx in (C). B and D, The graphs show percent (%) fibrotic area in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B and D). ##P<0.01 versus PBMNCTx in (B). #P<0.05 versus eEPCTx in (D). Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
Mentions: To evaluate anti‐inflammatory and ‐fibrotic potential of QQMNCTx, fibrotic area in ischemic ATM was detected by Azan staining on day 21 after treatment. The mean fibrotic area (% fibrotic area/×40 HPF) for the QQMNCTx, PBMNCTx, and control groups were 2.78±0.61, 8.41±1.51, and 11.94±3.59. These findings indicate that QQMNCTx exerted greater antifibrotic effects than did PBMNCTx (Figure 9A and 9B).

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH
Related in: MedlinePlus